Sex-biased single-cell genetic landscape in mice with autism spectrum disorder

作     者：Qian Zhang Yongjie Wang Jie Tao Ruixue Xia Yijie Zhang Zhirui Liu Jiwei Cheng Qian Zhang;Yongjie Wang;Jie Tao;Ruixue Xia;Yijie Zhang;Zhirui Liu;Jiwei Cheng

作者机构：Department of Neurology and Central LaboratoryPutuo HospitalShanghai University of Traditional Chinese MedicineShanghai 200062China School of PharmacyHangzhou Normal UniversityHangzhouZhejiang 311121China Department of Respiratory and Critical Care MedicineHenan University Huaihe HospitalKaifengHenan 475099China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2024年第51卷第3期

页      面：338-351页

核心收录：

学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(82074162 and 82274344) Project for Capacity Promotion of Putuo District Clinical Special Disease“Stroke”,Science and Technology Innovation Project of Putuo District Health System(ptkwws201902 and ptkwws202301) Training Plan of“100 professionals”of Shanghai Putuo District Central Hospital(2022-RCJC-05) Project of“XingLin Scholars Training”of Chengdu University of Traditional Chinese Medicine(YYZX2022170) Shanghai Putuo District Health System Clinical Characteristic Special Disease Construction Project(2023tszb04) 

主　　题：Autistic spectrum disorder(ASD) Sex Single cell Neurons Microglia 

摘      要：Autistic spectrum disorder(ASD)is a male-biased,heterogeneous neurodevelopmental disorder that affects approximately 1%e2%of the *** exposure to valproic acid(VPA)is a recognized risk factor for ASD,but the cellular and molecular basis of VPA-induced ASD at the single-cell resolution is ***,we aim to compare the cellular and molecular differences in the hippocampus between male and female prenatal mice with ASD at the single-cell transcriptomic *** transcriptomes of more than 45,000 cells are assigned to 12 major cell types,including neurons,glial cells,vascular cells,and immune *** type-specific genes with altered expression after prenatal VPA exposure are analyzed,and the largest number of differentially expressed genes(DEGs)are found in neurons,choroid plexus epithelial cells,and *** microglia,several pathways related to inflammation are found in both males and females,including the tumor necrosis factor(TNF),nuclear factor kappa B(NF-kB),toll-like receptor(TLR),and mitogen-activated protein kinase(MAPK)signaling pathways,which are important for the induction of autistic-like ***,we note that several X-linked genes,including Bex1,Bex3,and Gria3,were among the male-specific DEGs of *** pioneering study describes the landscape of the transcriptome in the hippocampus of autistic *** elucidation of sexual differences could provide innovative strategies for the prevention and treatment of ASD.