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China Medical Abstracts(Internal Medicine) 2016年第1期33卷 36-37页

作者：王悦 Tianjin Key Lab Cell & Molecul Immunol Dept ImmunolSch Basic Med SciTianjin Med Univ

Objective To investigate the proliferation,activation and cytokine production ofγδT cells during different periods of Chlamydia muridarum(Cm)respiratory tract infection.Methods C57BL/6 mice were inoculated intranasa... 详细信息

关键词： Chlamydia and IL-17 secretion cytokine inclusion inoculated murine cytometry producing chlamydia

EFFECTS OF(D-Arg^5)-t-GnRH-A AND DOMPERIDONE ON GONADOTROPIN secretion AND OVULATION IN COMMON CARP(Cyprinus carpio)

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Chinese Science Bulletin 1989年第4期34卷 350-351页

作者：林浩然梁坚勇 GLEN VAN DER KRAAK RICHARD E.PETER Department of Biology Zhongshvn University Guangzhou Department of Zoology University of Alberta Canada

It has been demonstrated that gonadotropin secretion in some teleosts is regulated by the actions of a gonadotropin releasing hormone (GnRH) and dopamine acting as a gonadotropin release-inhibitory factor (GRIF). The primary structure of a GnRH in chum salmon has been determined as (Trp^7,Leu^8)-LHRH. Further studies have demonstrated that this form of GnRH is found in a wide phylogenetic range of teleost, so it is a teleost GnRH (t-GnRH). The synthetic t-GnRH analogue, such as (D-Arg^6,

关键词： gonadotropin dopamine secretion Cyprinus analogue releasing salmon LHRH phylogenetic dosage

Effects of 3-methyladenine on airway inflammation,airway hyperresponsiveness and mucus secretion in asthmatic mice

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China Medical Abstracts(Internal Medicine) 2019年第2期36卷 98-99页

作者：冉琴 Dept Respir Dis Affil Hosp Southwest Med Univ

Objective To investigate the effects of 3-methyladenineon airway inflammation,airway hyperresponsivenessand mucus secretion in asthmatic mice,and to explore itsmechanism. Methods C57BL /6J female mice were randomlydivided into normal control group ( PBS),OVAgroup(OVA),OVA with 3-methyladenine group (OVA +3-MA),and OVA with 4-phenylbutyrate group (OVA +4-PBA). OVA group,OVA + 3-MA group and OVA + 4-PBA groups were all sensitized and challenged with OVAto establish asthmatic models,while PBS group was givenPBS as a control. At 2 h before challenge,OVA + 3-MAgroup was intraperitoneally injected with 3-methyladenine,and OVA + 4-PBA group was intraperitoneally injectedwith 4-phenylbutyrate. Airway hyperresponsiveness,eosinophils,and pathological changes of pulmonarytissue (hematoxylin-eosin,HE staining) were measuredto confirm the establishment of asthmatic models.

关键词： OVA PBA Effects of 3-methyladenine on airway inflammation,airway hyperresponsiveness and mucus secretion in asthmatic mice

EFFECTS OF LHRH-A AND DRUGS WHICH INHIBIT THE SYNTHESIS OF DOPAMINE ON GtH secretion AND OVULATION IN LOACH

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Chinese Science Bulletin 1986年第7期31卷 503-504页

作者：林浩然彭纯 Department of Biology Zhongshan UniversityGuangzhou Department of Biology

Gonadotropin secretion in teleosts is regulated by a gonadotropin releasing hormone (GnRH) and gonadotropin release-inhibitory factor (GRIF). In goldfish, dopamine functions as a GRIF. Our previous studies have shown that pimozide,a dopamine antagonist, potentiates the GtH release and ovulatory response to LHRH-A in the loach (Paramisgurnus dabryanus), thus indicating that dopamine probably also functions as a GRIF in loach.

关键词： dopamine gonadotropin secretion LHRH antagonist probably releasing alone terminals eater

Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture

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Journal of Animal Science and Biotechnology 2016年第4期7卷 504-513页

作者： Susumu Muroya Tatsuro Hagi Ataru Kimura Hisashi Aso Masatoshi Matsuzaki Masaru Nomura Animal Products Research Division NARO Institute of Livestock and Grassland Science Faculty of Agriculture and Life Science Hirosaki University International Education and Research Center for Food and Agricultural Immunology(CFAI) Graduate School of Agricultural ScienceTohoku University

Background： Bovine milk contains not only a variety of nutritional ingredients but also microRNAs （miRNAs） that are thought to be secreted by the bovine mammary epithelial cells （BMECs）. The objective of this study was to elucidate the production of milk-related miRNAs in BMECs under the influence of lactogenic hormones. Results： According to a microarray result of milk exosomal miRNAs prior to cellular analyses, a total of 257 miRNAs were detected in a Holstein cow milk. Of these, 18 major miRNAs of interest in the milk were selected for an expression analysis in BMEC culture that was treated with or without dexamethasone, insulin, and prolactin （DIP） to induce a lactogenic differentiation. Quantitative polymerase chain reaction （qPCR） results showed that the expressions of miR-21-Sp （P = 0.005）, miR-26a （P = 0.016）, and miR-320a （P = 0.011） were lower in the DIP-treated cells than in the untreated cells. In contrast, the expression of miR-339a （P-- 0.017） in the cell culture medium were lower in the DiP-treated culture than in the untreated culture. Intriguingly, the miR-148a expression in cell culture medium was elevated by DIP treatment of BMEC culture （P = 0.018）. The medium-to-cell expression ratios of miR- 103 （P = 0.025）, miR-148a （P 〈 0.001）, and miR-223 （P = 0.013） were elevated in the DIP-treated BMECs, suggesting that the lactogenic differentiation-induced secretion of these three miRNAs in BMECs. A bioinformatic analysis showed that the miRNAs down-regulated in the BMECs were associated with the suppression of genes related to transcriptional regulation, protein phosphorylation, and tube development. Conclusion： The results suggest that the miRNAs changed by lactogenic hormones are associated with milk protein synthesis, and mammary gland development and maturation. The elevated miR-148a level in DIP-treated BMECs may be associated with its increase in milk during the lactation period of cows.

关键词： Bovine mammary epithelial cell Lactogenic differentiation microRNA Milk secretion

Morphology of platelet Golgi apparatus and their significance after acute cerebral infarction

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Neural Regeneration Research 2013年第23期8卷 2134-2143页

作者： Wei Lu Dong Xu Ranran Tu Zhiping Hu Department of Neurology Second Xiangya HospitalCentral South University

Blood samples were harvested from the antecubital vein of 20 fasting patients with acute cerebral infarction at 1, 7 and 15 days after onset to prepare blood platelet suspension. Fasting antecubital vein blood was collected from an additional 20 normal adults as controls. Under transmission elec- tron microscope, platelet Golgi tubules and vesicles became significantly thickened, enlarged, and irregular after acute cerebral infarction. Alpha granules in platelets significantly reduced in number, especially 1 day after cerebral infarction. Under immunoelectron microscopy, a few alpha granules aggregated around Golgi tubules and vesicles after infarction. These results suggested that platelet Golgi apparatus displayed significant morphological changes, which were possibly associated with enhanced synthetic and secretory functions of activated platelets after acute cerebral infarction. This study used Golgi apparatus blocking agent Brefeldin A to block Golgi apparatus in an aim to study the effects of Golgi apparatus on CD40L expression on the surface of activated platelets. Flow cytometry revealed that CD40L expression on activated platelet surfaces decreased significantly when Golgi apparatus was blocked, which indicated that Golgi apparatus participated in the syn- thesis and transport of CD40L to the platelet surface.

关键词： neural regeneration brain injury Golgi apparatus CD40L alpha granules platelet ultrastructure cerebral infarction transport secretion Brefeldin A grants-supported paper neuroregeneration

EFFECTS OF ATRIAL NATRIURETIC FACTOR ON PATIENTS WITH RENOVASCULAR HYPERTENSION

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中华医学杂志(英文版) 1992年第9期105卷 732-737页

作者：张卫国刘力生郑德裕刘国杖金律赵秀文任兵 Department of Hypertension Cardiovascular Institute and Fu Wai Hospital Chinese Academy of Medical Sciences Beijing 100037

Previous studies have shown the effects of atrialnatriuretic factor(ANF)on rats with renovascularbypertension(RVH).In the present study low doseα-hANF(0.025μg/kg/min)was administeredintravenously for 60 minutes to seven RVH patients.Results demonstrated an inhibition of renin-angiotensin-aldosterone system(RAAS),reduction ofplasma catecholamine and arginine vasopressin(AVP),diuresis and natriuresis,increase of hematocrit andcreatinine clearance,and slight decrease of blood pres-sure.These results showed that most factors involvedin the establishment and maintenance of RVH are af-fected by ANF infusion.

关键词： CHRONIC INFUSION BLOOD-PRESSURE PLASMA-RENIN 2-KIDNEY PEPTIDE ALDOSTERONE secretion SODIUM

LORELEI-LIKE GPI-ANCHORED PROTEINS 2/3 Regulate Pollen Tube Growth as Chaperones and Coreceptors for ANXUR/BUPS Receptor Kinases in Arabidopsis

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Molecular Plant 2019年第12期12卷 1612-1623页

作者： Hanqian Feng Chen Liu Rong Fu Minmin Zhang Hui Li Lianping Shen Qiqi Wei Xiang Sun Lin Xu Bin Ni Chao Li School of Life Sciences East China Normal UniversityShanghai 200241China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of Sciences300 Fenglin RoadShanghai 200032China

Pollen tube growth is crucial for successful fertilization.In Arabidopsis thaliana,the ANXUR(ANX)/BUPS receptor kinase complex controls and maintains pollen tube growth in response to autocrine rapid alkalin-ization factor 4/19(RALF4/19)signaling;however,the molecular and cellular mechanisms underlying the ANX/BUPS-mediated regulation of pollen tube growth remain unclear.In this study,we found that pollen-specific LORELEI-like GPI-anchored proteins 2 and 3(LLG2/3)promote pollen tube growth in vitro and in vivo.LLG2/3 interacte with ANX/BUPS in a RALF4-concentration-dependent manner,suggesting that ANX/BUPS and LLG2/3 might form a receptor-coreceptor complex for perceiving RALF peptide signals.Disruption of the ANX/BUPS-LLG2/3 interaction led to the cytoplasmic retention of ANX1/2,in either llg2/3 knockdown mutants or in anx1/2 mutants lacking the J region,which mediates the ANX/BUPS-LLG2/3 interaction.Moreover,we found that RALF4 induced the production of reactive oxygen species(ROS),which stimulate pollen tube growth and reduce pollen burst rate.ROS levels are reduced in the pollen tubes of LLG2/3 RNAi lines,and application of exogenous H2O2 could partially rescue the defective pollen tube growth of LLG2/3 RNAi lines.Taken together,our study identifies LLG2/3 as novel regulatory components of pollen tube growth,and shows that they chaperone ANX/BUPS for secretion to the apical plasma membrane of pollen tube and act as coreceptors of ANX/BUPS in the acti-vation of ROS production for promoting pollen tube growth.

关键词： reception complex secretion reactive oxygen species cell wall integrity

Human umbilical cord blood stem cell transplantation for the treatment of chronic spinal cord injury Electrophysiological changes and long-term efficacy

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Neural Regeneration Research 2013年第5期8卷 397-403页

作者： Liqing Yao Chuan He Ying Zhao Jirong Wang Mei Tang Jun Li Ying Wu Lijuan Ao Xiang Hu Department of Rehabilitation Second Affiliated Hospital of Kunming Medical University Shenzhen Beike Cell Engineering Research Institute

Stem cell transplantation can promote functional restoration following acute spinal cord injury （injury time 〈 3 months）, but the safety and long-term efficacy of this treatment need further exploration. In this study, 25 patients with traumatic spinal cord injury （injury time 〉 6 months） were treated with human umbilical cord blood stem cells via intravenous and intrathecal injection. The follow-up period was 12 months after transplantation. Results found that autonomic nerve functions were restored and the latent period of somatosensory evoked potentials was reduced. There were no severe adverse reactions in patients following stem cell transplantation. These experimental findings suggest that the transplantation of human umbilical cord blood stem cells is a safe and effective treatment for patients with traumatic spinal cord injury

关键词： neural regeneration spinal cord injury human umbilical cord blood stem cells transplantation paraplegia American Spinal Cord Injury Association score neurological function secretion somatosensory evoked potentials spasm safety photographs-containing paper neurogeneration

Taraxastane-type triterpenoids from the medicinal and edible plant Cirsium setosum

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Chinese Journal of Natural Medicines 2019年第1期17卷 22-26页

作者： LIN Peng-Cheng JI Lin-Lin ZHONG Xiang-Jian LI Jin-Jie WANG Xin SHANG Xiao-Ya LIN Sheng College of Pharmaceutical Sciences Qinghai University for Nationalities Beiijing Key Laboratory of Bioactive Substances and Functional Foods Beijing Union University State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College

Guided by TNF-α secretion inhibitory activity assay, four taraxastane-type triterpenoids, including two new ones, 22-oxo-20-taraxasten-3β, 30-diol(1) and 22α-hydroxy-20-taraxasten-30β, 30-triol(2), have been obtained from an active fraction of the petroleum ether-soluble extract of the the medicinal and edible plant Cirsium setosum. Their structures were elucidated by spectroscopic data and CD data analysis. In the TNF-α secretion inhibitory activity assay, compounds 1 and 2 were active with the IC50 of 2.6 and 3.8 μmol·L-1, respectively. In addition, compounds 1 and 2 showed moderately selective cytotoxicity against the human ovarian cancer(A2780) and colon cancer(HCT-8) cell lines.

关键词： Cirsium setosum Taraxastane-type triterpenoid TNF-α secretion inhibitory activity Cytotoxicity