Morphology of platelet Golgi apparatus and their significance after acute cerebral infarction

作     者：Wei Lu Dong Xu Ranran Tu Zhiping Hu 

作者机构：Department of NeurologySecond Xiangya HospitalCentral South University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2013年第8卷第23期

页      面：2134-2143页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China, No.81171239/H0914 Frontier Research Key Project,Central South University in China (2010-2011), No.2177-721500065 the Education Expenditure of Hunan Provincial Finance Department in China, No.2010163 

主　　题：neural regeneration brain injury Golgi apparatus CD40L alpha granules platelet ultrastructure cerebral infarction transport secretion Brefeldin A grants-supported paper neuroregeneration 

摘      要：Blood samples were harvested from the antecubital vein of 20 fasting patients with acute cerebral infarction at 1, 7 and 15 days after onset to prepare blood platelet suspension. Fasting antecubital vein blood was collected from an additional 20 normal adults as controls. Under transmission elec- tron microscope, platelet Golgi tubules and vesicles became significantly thickened, enlarged, and irregular after acute cerebral infarction. Alpha granules in platelets significantly reduced in number, especially 1 day after cerebral infarction. Under immunoelectron microscopy, a few alpha granules aggregated around Golgi tubules and vesicles after infarction. These results suggested that platelet Golgi apparatus displayed significant morphological changes, which were possibly associated with enhanced synthetic and secretory functions of activated platelets after acute cerebral infarction. This study used Golgi apparatus blocking agent Brefeldin A to block Golgi apparatus in an aim to study the effects of Golgi apparatus on CD40L expression on the surface of activated platelets. Flow cytometry revealed that CD40L expression on activated platelet surfaces decreased significantly when Golgi apparatus was blocked, which indicated that Golgi apparatus participated in the syn- thesis and transport of CD40L to the platelet surface.