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Aberrant expression of nuclear matrix proteins during HMBA-induced differentiation of gastric cancer cells

World Journal of Gastroenterology 2010年第17期16卷 2176-2182页

作者： Jing, Guang-Jun Xu, Dong-Hui Shi, Song-Lin Li, Qi-Fu Wang, San-Ying Wu, Fu-Yun Kong, Hai-Yan Xiamen Univ Key Lab Minist Educ Cell Biol & Tumor Cell Engn Sch Life Sci Xiamen 361005 Fujian Province Peoples R China Xiamen Univ Dept Hepat Biliary Pancreat Vasc Surg Affiliated Hosp 1 Xiamen 361003 Fujian Province Peoples R China

AIM: To investigate the aberrant expression of nuclear matrix proteins in human gastric cancer cells before and after hexamethylene bisacetamide (HMBA) ***: Proteomics analysis of differential nuclear matrix proteins was performed by two dimensional electrophoresis polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass *** expression levels of three nuclear matrix proteins were further confirmed by Western blotting and their locations in nuclear matrix filament were observed by quantum dots-based ***: Proteomics analysis showed that 43 protein spots were significantly changed due to HMBA *** proteins were identified in the HMBAinduced differentiation of gastric tumor *** proteins spots were down-regulated while seven were *** these proteins,prohibitin,nucleophosmin and hnRNP A2/B1 were significantly decreased in HMBA-treated human gastric cancer cells,and their locations in nuclear matrix were altered by *** results proved the alteration of specific nuclear matrix proteins during the differentiation of human gastric cancer *** the aberrant expressions of nuclear matrix proteins were of significance in revealing the regulatory mechanism of tumor cell proliferation and ***: The aberrant expressions and intracellular redistributions of nuclear matrix proteins before and after HMBA treatment indicated that nuclear matrix proteins play a pivotal role in the differentiation of gastric cancer cells.

关键词： Human gastric tumor cell Hexamethylene bisacetamide Differentiation nuclear matrix Proliferation

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Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum

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Cell Research 1999年第1期9卷 61-69页

作者： ZENG XIAN LU MING DA JIAO MIAO XING XIAO GUANG WANG SHUI HAO(Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China)(Department of Biology, Changchun Normal College,Changchun 130032, China) Institute of Genetics and Cytology Northeast Normal University Changchun China Department of Biology Changchun Normal College Changchun China

The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrir and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaCl. SDS-PAGE analyses revealed that the nuclear matrir and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labeled with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescence, suggesting the presence of the antigen in *** results confirmed the presence of tropomyosin in the nuclear matrix and chromosome scaffold. Immunoelectron microscopic obserwtions further demonstrated that tropomyosin was dispersively distributed in the interphase nuclei and metaphase chromosomes.

关键词： Chromosome scaffold nuclear matrix Physarum polycephalum tropomyosin

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Association of DNA with nuclear matrix in in vitro assembled nuclei induced by rDNA from Tetrahymena shanghaiensis in Xenopus egg extracts

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Cell Research 1997年第1期7卷 107-117页

作者： CHEN YING BO ZHANG XIU FEN LI ZHONG HE ZHAI(Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871) Department of Cell Biology and Genetics College of Life Sciences Peking University Beijing

The nuclei assembled from exogenous DNA or chromatin in egg extracts resemble their in vivo counterparts in many aspects. However, the distribution pattern of DNA in these nuclei remains unknown. We introduced rDNA from the macronuclei of Tetrabymena into Xenopus cellfree extracts to examine the association of specific DNA sequences with nuclear matrix (NM) in the nuclei assembled in vitro. Our previous works showed the 5'NTS (nontranscription sequences) of the rDNA specifically bind to the NM system in the macronuclei. We show now the rDNA could induce chromatin assembly and nuclear formation in Xenopus cell-free system. When we extracted the NM system and compared the binding affinity of different regions of rDNA with the NM system, we found that the 5'NTS still hold their binding affinity with insoluble structure of the assembled nuclei in the extracts of Xenopus eggs.

关键词： nuclear assembly nuclear matrix Xenopus egg extracts Tetrahymena rDNA

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nuclear matrix associated protein PML: an arsenic trioxide apoptosis therapeutic target protein in HepG2 cells

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Chinese Medical Journal 2003年第1期116卷 93-98页

作者：于鼎王子慧朱立元邱殷庆 Department of Surgery Department of Pathology Department of Anatomy The Chinese University of Hong Kong Hong Kong China

Objective To investigate arsenic trioxide (As 2O 3)-induced apoptosis and the effects on cell nuclear matrix related protein promyelocytic leukaemia (PML). Methods HepG2 cells were cultured in MEM medium and treated with 0.5, 2, 5 and 10 μmol/L As 2O 3 for either 24 h or 96 h at each concentration. In situ terminal deoxynucleotidyl transferase (TdT) labeling (TUNEL) and DNA ladders were used to detect apoptosis. Confocal microscopy and Western blotting were used to observe the expression of PML. Results The growth rates of HepG2 cells were slower in the As 2O 3 treated than the untreated control group. DNA ladder and TUNEL positive apoptotic cells could be detected in As 2O 3 treated groups. The expression of PML decreased in HepG2 cells with 2 μmol/L As 2O 3 treatment. Confocal images demonstrated that the expression of PML protein in HepG2 cell nuclei decreased after treatment with 2 μmol/L As 2O 3, and micropunctates characteristic of PML protein in HepG2 cell nuclei disappeared after treatment with 5 μmol/L As 2O *** Our results show that arsenic trioxide can significantly inhibit the growth of HepG2 cells in vitro. As 2O 3 induces apoptosis in HepG2 tumor cells in a time and concentration dependent manner. As 2O 3 may degrade the PML protein in HepG2 cell nuclei. The decreased expression of PML in As 2O 3 treated tumor cells is most likely to be caused by apoptosis. nuclear matrix associated protein PML could be the target of As 2O 3 therapy.

关键词： nuclear matrix arsenic trioxide PML protein apoptosis HepG2 cell line

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Phosphorylation and biosynthesis of high molecular weight proteins of tumor nuclear matrix

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Cell Research 1998年第3期8卷 195-207页

作者： BAZARNOVA TM TV BULDYAEVA LS FILATOVA SB AKOPOV IB ZBARSKY(NK Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Street, 117344 Moscow,Russia) NK Koltzov Institute of Developmental Biology Russian Academy of Sciences Moscow Russia

Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. contrary to normal cells, Proteins of this group are Preferentially phosphorylated. Phosphoproteins of hepatoma nuclear matrix are selectively subjected to rapid proteolysis. By alkali treatment and a monoclonal antibody against phosphotyrosyl residue the presence of two high molecular weight bands of phosphotyrosyl-containing proteins was defected in nuclear matrices of tumor but not of normal liver cells. High molecular weight protein group of tumor nuclear matrices revealed also a rapid turnover and preferential incorporation of labeled amino acids selectively inhibited by chioramphenicol.

关键词： Tumors nuclear matrix phosphoproteins phosphotyrosine nuclear protein biosynthesis

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On the history of nuclear matrix manifestation

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Cell Research 1998年第2期8卷 99-103页

作者： ZBARSKY IB(NK. Koltzov Institute of Developmental Biology, Russian Academy of Sciences. 26, Vavilov Street, 117334 Moscow,Russia. e-mail: ibzba ibrran.msk-su) N.K. Koltzov Institute of Developmental Biology Russian Academy of Sciences Moscow Russia

The nonchromatin proteinous residue of the cell nucleus was revealed in our laboratory as early aJs in 1948and then identified by light and electron microscoPy as residual nucleoli. intranuclear network and nuclear envelope before 1960. This structure termed afterwards as 'nuclear residue', 'nuclear skeleton', 'nuclear cage' 3 'nuclearcarcass' etc., was much later (in 1974) isolated, studied,and entitled as 'nuclear matrix' by Berezney and Coffey,to whom the discovery of this residual structure is of ten wronly ascribed. The real history of nuclear matrix manifestation is reported in this paper.

关键词： nuclear matrix nuclear residue nuclear fractions history of nuc1ear fractionation

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The detection of nuclear matrix in most primitive presentexisting eukaryote, Giardia lamblia

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Cell Research 1995年第2期5卷 273-278页

作者： DAI JIALING JINGYAN LI SIQI LU(Laboratory of Evolutionary Cell Biology, Kunming Institute of Zoology, Academia Sinica, Kunming, Yunnan 650223, China.)(Capital Institute of Medicine, Beijing 100054, China.) Laboratory of Evolutionary Cell Biology Kunming Institute of Zoology Academia Siniea Kunmiug China Capital Institute of Medicine Beijing China

The nuclear matrix of diplomonad Giardia lamblia was detected for the first time with DGD embedmentsectioning- embedment free electron microscopy after a series of specific extractions. The result showed that archaezoa Giardia lamblia already possessed nuclear matrix within its two nuclei. The finest fibrils of the nuclear matrix of Giardia lamblia were measured to be about 11 to 13 nm in thickness. However, the nuclear lamina and nucleolus have never been observed. These results seem to suggest that nuclear matrix is an indispensable intranuclear structural component even in the primitive nucleus.

关键词： Archezoa Giardia lamblia nuclear matrix nuclear lamina nucleolus

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The interaction between the human β-globin locus control region and nuclear matrix

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Cell Research 2002年第Z2期 411-416页

作者： SHU BING ZHANG, Ruo LAN QIANState Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China

Our previous study showed that hydroxyurea (Hu) could induce HEL cells to express humanβ-globin gene. However the molecular mechanisms by which the expression of β-globin gene is activated and regulated are poorly understood. Here we show that the binding patterns between the core DNA sequences (HS2 core sequence -10681- -10971 bp , HS3 core sequence -14991- -14716 bp and HS4 core sequence -18586- -18306 bp) of DNase I hypersensitive sites in the human β-globin LCR and nuclear matrix proteins isolated from Hu induced and uninduced HEL cells are quite different. Results demonstrated that nuclear matrix proteins might play important roles in regulating the expression of humanβ-like globin genes through their interaction with HSs (HS2,HS3 and HS4 core sequences) in the LCR. Moreover, the results obtained from the in vitro DNA-matrix binding assay showed that the core DNA sequences of DNase I hypersensitive sites (HS2, HS3 and HS4) were unable to bind to the nuclear matrix isolated from uninduced HEL cel

关键词： human β-globin LCR nuclear matrix proteins nuclear matrix HEL cells.

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Identification of the nuclear matrix and chromosome scaffold in dinoflagellate Crythecodinium cohnii

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Cell Research 1992年第2期2卷 165-181页

作者： CAT SHUTAO , CONGMEI ZENG, JINGYAN LI AND ZHONGHE ZHAI Department of Biology, Beijing University, Beijing 100871, China. Kunming Institute of Zoology, Academia Sinica, Kunming 650107, China. Department of Biology Beijing University Beijing China Kunming Institute of Zoology Academia Sinica Kunming China

Dinoflagellate is one of the primitive eukaryotes,whosenucleus may represent one of the transition stages fromprokaryotic nucleoid to typical eukaryotic *** extraction together with embeddment-free sectionand whole mount electron microscopy,a delicate nuclearmatrix filament network was shown,for the first time,indinoflagellate Crypthecodinium cohnii *** are connected with nuclear matrix filaments to forma complete network spreading over the ***,we demonstrated that the dinoflagellate chromosome retainsa protein scaffold after the depletion of DNA and *** scaffold preserves the characteristic mor-phology of the *** dimensional elec-trophoreses indicated that the nuclear matrix and chromo- some scaffold are mainly composed of acidic *** demonstrated that a framework similar to the nuclearmatrix and chromosome scaffold in mammalian cells appearsin this primitive eukaryote,suggesting that these structuresmay have been originated from the early stages of eukaryoteevolution.

关键词： nuclear matrix chromosome scaffold dinoflagellate Crypthecodinium cohnii.

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nuclear matrix-lamina-intermediate filament system in eukaryotic cells

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Progress in Natural Science:Materials International 1996年第1期 8-13页

作者：焦仁杰 Beijing 100871 China College of Life Science Peking University

In the past twenty years, cell biologists have identified a new cell structure, nuclear matrix or nuclear skeleton, which was found in the nucleus after removing histones and chromatin carrying on genetic information. nuclear matrix is mainly composed of insoluble fibrous proteins which construct a network maintaining the morphology of the *** plays an important role in cell metabolic activities such as DNA replication, gene expression, cell differentiation etc. and cancer therapy. nuclear lamina is a 10 nm fibers network lying under the eukaryotic nuclear membranes. It is also related to cell division, cell differentiation, nuclear envelope construction, chromatin reassembly and nuclear

关键词： nuclear matrix nuclear lamina intermediate filament.

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