Nuclear matrix associated protein PML: an arsenic trioxide apoptosis therapeutic target protein in HepG2 cells

作     者：于鼎 王子慧 朱立元 邱殷庆 

作者机构：Department of Surgery Department of Pathology Department of Anatomy The Chinese University of Hong Kong Hong Kong China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2003年第116卷第1期

页      面：93-98页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：nuclear matrix arsenic trioxide PML protein apoptosis HepG2 cell line 

摘      要：Objective To investigate arsenic trioxide (As 2O 3)-induced apoptosis and the effects on cell nuclear matrix related protein promyelocytic leukaemia (PML). Methods HepG2 cells were cultured in MEM medium and treated with 0.5, 2, 5 and 10 μmol/L As 2O 3 for either 24 h or 96 h at each concentration. In situ terminal deoxynucleotidyl transferase (TdT) labeling (TUNEL) and DNA ladders were used to detect apoptosis. Confocal microscopy and Western blotting were used to observe the expression of PML. Results The growth rates of HepG2 cells were slower in the As 2O 3 treated than the untreated control group. DNA ladder and TUNEL positive apoptotic cells could be detected in As 2O 3 treated groups. The expression of PML decreased in HepG2 cells with 2 μmol/L As 2O 3 treatment. Confocal images demonstrated that the expression of PML protein in HepG2 cell nuclei decreased after treatment with 2 μmol/L As 2O 3, and micropunctates characteristic of PML protein in HepG2 cell nuclei disappeared after treatment with 5 μmol/L As 2O *** Our results show that arsenic trioxide can significantly inhibit the growth of HepG2 cells in vitro. As 2O 3 induces apoptosis in HepG2 tumor cells in a time and concentration dependent manner. As 2O 3 may degrade the PML protein in HepG2 cell nuclei. The decreased expression of PML in As 2O 3 treated tumor cells is most likely to be caused by apoptosis. Nuclear matrix associated protein PML could be the target of As 2O 3 therapy.