Association of DNA with nuclear matrix in in vitro assembled nuclei induced by rDNA from Tetrahymena shanghaiensis in Xenopus egg extracts
Association of DNA with nuclear matrix in in vitro assembled nuclei induced by rDNA from Tetrahymena shanghaiensis in Xenopus egg extracts作者机构：Department of Cell Biology and Genetics College of Life Sciences Peking University Beijing
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：1997年第7卷第1期
页 面：107-117页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
基 金：National Natural Sciences Foundation of China
主 题：Nuclear assembly nuclear matrix Xenopus egg extracts Tetrahymena rDNA
摘 要：The nuclei assembled from exogenous DNA or chromatin in egg extracts resemble their in vivo counterparts in many aspects. However, the distribution pattern of DNA in these nuclei remains unknown. We introduced rDNA from the macronuclei of Tetrabymena into Xenopus cellfree extracts to examine the association of specific DNA sequences with nuclear matrix (NM) in the nuclei assembled in vitro. Our previous works showed the 5 NTS (nontranscription sequences) of the rDNA specifically bind to the NM system in the macronuclei. We show now the rDNA could induce chromatin assembly and nuclear formation in Xenopus cell-free system. When we extracted the NM system and compared the binding affinity of different regions of rDNA with the NM system, we found that the 5 NTS still hold their binding affinity with insoluble structure of the assembled nuclei in the extracts of Xenopus eggs.
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