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Mulberry leaf flavonoids protect against glucotoxicity-induced INS-1 cell apoptosis

Journal of Traditional Chinese Medicine 2019年第2期39卷 153-159页

作者： Wang Ying Li Ming Yu Xuesong He Sheng Wu Xinrong Wang Yan School of Chemistry Engineering Guangdong Pharmaceutical University School of Biosciences and Biopharmaceutics Guangdong Pharmaceutical University Department of Pharmacy General Hospital of Guangzhou Military Command of People's Liberation Army School of Traditional Chinese Medicine Guangdong Pharmaceutical University

OBJECTIVE:To investigate the effect of mulberry leaf flavonoids (MLF) on apoptosis of pancreatic cells induced by high ***:Long exposure to high glucose induces apoptosis of pancreaticβcells, which can lead to diabetes. In this study, we used the rat insulinoma cell line, INS-1. High glucose (33.3 mM) was used to establish a glucotoxicity model. The MTT assay was used to evaluate the MLF effect on cell ***-1 cells were treated with various concentra-tions of MLF (125, 250 and 500 mg/L) for 24 h, and then stimulated with 5.5 or 33.3 mM glucose for 48 ***, the cell supernatants were collected for enzyme-linked immunosorbent assay to determine the level of superoxide dismutase (SOD), catalase(CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), monocyte chemoattractant protein1 (MCP-1), tumor necrosis factor a (TNF-α) and interleukin 6 (IL-6). Western blotting was used to determine the expression of Bcl-2, Bax, caspase-3 and Caspase-9. Cell apoptosis was measured by Annexin V-FITC/propidium iodide double staining and flow ***:MLF (125-500 mg/L) improved cell viability. Furthermore, MLF (250 and 500 mg/L) inhibited apoptosis induced by high glucose. The anti-apoptosis effect of MLF was associated with increased SOD, CAT and GSH-Px expression, as well as reduced MDA levels in high-glucose-treated INS-1cells. Moreover, MLF upregulated Bcl-2 expression,downregulated Bax expression, and reduced the expression of caspase-3 and Caspase-9. Finally, MLF decreased the secretion of inflammatory cytokines and insulin in high-glucose-induced INS-1 ***:MLF is a potential therapeutic agent for preventing diabetes and related disorders.

关键词： Morus Plant leaves In suli no ma Flavon oids Toxicity Apoptosis

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Effects of 1-Methylcyclopropene on no Content,noS Activity,and H_2O_2 Content in Postharvest Suli Pears

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Agricultural Sciences in China 2011年第5期10卷 797-804页

作者： DONG Yu LIU Li-qin GUAN Jun-feng Institute of Genetics and Physiology Hebei Academy of Agricultural and Forestry Sciences Shijiazhuang 050051 P.R.China College of Life Science Hebei Normal University Shijiazhuang 050016 P.R.China

Studies were conducted over the effects of 1-methylcycolpropene （1-MCP） treatment on postharvest life of Suli pears （Pyrus bretschneideri Rehd.） stored at room and cold temperatures on nitric oxide （no）,nitric oxide synthase （noS） activity,and hydrogen dioxide （H2O2）.Results showed that the 1-MCP treatment had little effect on total soluble solids （TSS） at both room and cold temperatures.1-MCP delayed softening of Suli pear fruits,decreased respiratory rate and H2O2 accumulation,and increased no and noS activity at room temperature storage,while the effect of cold temperature storage was relatively *** was a significant positive correlation between noS activity and no *** is concluded that 1-MCP had effects on endogenous no content and accumulation of ***,H2O2 acting as a signaling molecule via regulating no level affects the ripening and senescence of Suli pears.

关键词： pear 1-MCP no noS H2O2

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Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor

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Chinese Journal of Integrative Medicine 2020年第4期26卷 270-276页

作者： HAO Jian DING Xiu-li YANG Xue WU Xiong-zhi Zhong-Shan-Men In-Patient Department Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin(300060)China Clincal Center of Tumor Therapy The Fourth Central Hospital Affiliated to Nankai UniversityTianjin(300140)China

Objective:To study the effects of Prunella vulgaris polysaccharide(PVP)on human breast carcinoma-associated fibroblasts(CAFs).Method:Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)?2H?tetrazolium(MTS)*** healing experiment and transwell migration assay were used to investigate the anti-migration *** cytometry was applied to detect cell apoptosis and cell cycle *** transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor(bFGF)in *** SKBr-3 with CAFs conditioned medium(CAFs-CM)to evaluate the indirect function on the proliferation of breast cancer SKBr-3 ***:PVP inhibited the viability of CAFs by inducing apoptosis(P<0.01)and arresting cell cycle(P<0.01).It also inhibited the migration of CAFs(P<0.01).bFGF promoted CAFs proliferation(P<0.01)and migration(P<0.01),protected CAFs from apoptosis(P<0.05)and reduced Go phase to 49.06%(P<0.01).However,these effects of bFGF on CAFs could be abrogated by *** SKBr-3 with CAFs-CM,PVP could inhibit the viability of breast cancer SKBr-3 cells ***,PVP reduced the mRNA expression(P<0.01)and protein secretion of bFGF(P<0.01)in ***:PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expressi on,thus inhibit!ng the growth of breast can cer SKBr-3 cells in directly.

关键词： Prunella vulgaris polysaccharide carci no ma-associated fibroblasts basic fibroblast growth factor SKBr-3 Chinese medicine

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Plant peroxisomes at the crossroad of no and H2O2 metabolism

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Journal of Integrative Plant Biology 2019年第7期61卷 803-816页

作者： Francisco J.Corpas Luis A.del Río José M.Palma Group of Antioxidants Free Radicals and Nitric Oxide in Biotechnology Food and Agriculture Department of Biochemistry Cell and Molecular Biology of Plants Estación Experimental del Zaidín CSIC

Plant peroxisomes are subcellular compartments involved in many biochemical pathways during the life cycle of a plant but also in the mechanism of response against adverse environmental *** organelles have an active nitro-oxidative metabolism under physiological conditions but this could be exacerbated under stress ***,peroxisomes have the capacity to proliferateand also undergo biochemical adaptations depending on the surrounding cellular *** important characteristic of peroxisomes is that they have a dynamic metabolism of reactive nitrogen and oxygen species(RNS and ROS)which generates two key molecules,nitric oxide(no)and hydrogen peroxide(H2O2).These molecules can exert signaling functions by means of post-translational modifications that affect the functionality of target molecules like proteins,peptides or fatty *** review provides an overview of the endogenous metabolism of ROS and RNS in peroxisomes with special emphasis on polyamine and uric acid metabolism as well as the possibility that these organelles could be a source of signal molecules involved in the functional interconnection with other subcellular compartments.

关键词： Plant peroxisomes no H2O2 metabolism

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Application of Tungsten as a De-no_x Catalyst

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Rare Metals 1994年第3期13卷 229-235页

作者： Nakatsuji T.(Sakai Chemical Industry, Co. , LId. 5-I, Ebisujimacho Sakai Osako 590, Japan) Sakai Chemical Industry Co 日本

Abstract A catalyst for removal of nitrogen oxides, in stationary Sources, requires many kinds of characteristicsfor commercial application. At first, a vanadium pentaoxide based catalyst was used for the no-NH3-OZreaction because of its high activity at lower temperatures. But it was`later found that this catalyst had *** like a low thermal durability and a high conversion rate of SO: to SO3. On the other hand, tungstentrioxide was well known as a catalyst that has a high thermal durability and a low conversion rate of SOZto SO3 but showed a low activity at appropriate temperature. For these reasons, tungsten trioxide basedcatalysts have not been used in the reaction in most actual plants except for special cases. Further *** on this catalyst revealed that a catalyst comprising of titania f80 wt%￣ 90 wt%j- tungsten trioxide(5 wt% ￣ 15 wt%j, and vanadium pentaoxide (0,l wt%￣ 5 wt%j, showed a high thermal durability and alow conversion rate of SOZ to SO3 as well as high activity towards the no-NHrOz reaction.

关键词： no De

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Theoretical study on the reaction mechanism of (CH3)3CO(.) radical with no

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Science China Chemistry 2004年第4期47卷 289-294页

作者： ZHAO Hongmei1,2, LIU Kun1, SUN Chengke1 & LI Zonghe1 1. Department of Chemistry, Beijing normal University, Beijing 100875, China 2. Department of Chemistry, Beihua University, Jilin 132013, China 1. Department of Chemistry Beijing Normal University 100875 Beijing China 2. Department of Chemistry Beihua University 132013 Jilin China

The reaction mechanism of (CH3)3CO? radical with no is theoretically investigated at the B3LYP/6-31G* level. The results show that the reaction is multi-channel in the single state and triplet state. The potential energy surfaces of reaction paths in the single state are lower than that in the triple state. The balance reaction: (CH3)3COno(CH3)3CO?+no, whose potential energy surface is the lowest in all the reaction paths, makes the probability of measuring (CH3)3CO? radical increase. So no may be considered as a stabilizing reagent for the (CH3)3CO? radical.

关键词： (CH3)3CO. radical DFT reaction mechanism no

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Synthesis and anti-hepatocellular carcinoma activity of novel O^2-vinyl diazeniumdiolate-based nitric oxide-releasing derivatives of oleanolic acid

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Chinese Journal of Natural Medicines 2017年第12期15卷 928-937页

作者： ZOU Yu YAN Chang LIU Jing-Chao HUANG Zhang-Jian XU Jin-Yi ZHOU Jin-Pei ZHANG Hui-Bin ZHANG Yi-Hua State Key Laboratory of National Medicines Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases China Pharmaceutical University Nanjing 210009 China

Considering that high levels of nitric oxide(no) exert anti-cancer effect and the derivatives of oleanolic acid(OA) have shown potent anti-cancer activity, new O^2-vinyl diazeniumdiolate-based no releasing derivatives(5a–l, 11a–l) of OA were designed, synthesized, and biologically evaluated in the present study. These derivatives could release different amounts of no in liver cells. Among them, 5d, 5i, 5j, 11g, 11h, and 11j released more no in SMMC-7721 cells and displayed stronger proliferative inhibition against SMMC-7721 and Hep G2 cells than OA and other tested compounds. The most active compound 5j showed almost 20-fold better solubility than OA in aqueous solution, released larger amounts of no in liver cancer cells than that in normal ones, and exhibited potent anti-hepatocellular carcinoma activity but little effect on the normal liver cells. The inhibitory activity against the cancer cells was significantly diminished upon addition of an no scavenger, suggesting that no may contribute, at least in part, to the activity of 5j.

关键词： Oleanolic acid O^2-Vinyl diazeniumdiolate Cytochrome P450 no release Anti-hepatocellular carcinoma activity

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no_x Removal by Oxidation on Alumina Supported Metal Oxide Catalysts Followed by Alkaline Absorption

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China Petroleum Processing & Petrochemical Technology 2019年第3期21卷 1-9页

作者： Yang Xue Sun Min Liu Bo Lin Wei SInoPEC Research Institute of Petroleum Processing

no oxidation is the key reaction for the oxidative no x removal *** this work,the catalytic no oxidation performance of the Al2O3 supported metal oxide catalysts(M-Al2O3,M=V,Mn,Fe,Co,Ni and Ce)is *** oxidation product is absorbed by the alkaline solution for no x *** no oxidation activity increases in the following order:Vno oxidation involves the O uptake into the metal oxide lattice and oxidation of the adsorbed no by the lattice O,the highest activity of Mn is attributed to the appropriate redox potential of Mn,which favors both the O uptake and the no oxidation *** all the M-Al2O3 catalysts,there is an intermediate temperature to achieve maximum no conversion,which is lower for more efficient M-Al2O3 *** temperature dependence suggests that the no oxidation at low temperature is kinetically controlled while it is thermodynamically limited at higher *** no x removal ratio by the alkaline solution absorption increases with the no2/no ratio,with a maximum removal ratio of 80%when the no2/no ratio is higher than 3,indicating that a very high no conversion is unnecessary.

关键词： nox removal no oxidation M-Al2O3 catalysts alkaline absorption

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The Ab Initio Studies of no Chemisorption on TiO_2(110) Surface

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Chinese Journal of Structural Chemistry 1999年第4期18卷 304-309页

作者：吴立明章永凡等 DepartmentofChemistry FuzhouniversityFuzhouFujia DepartmentofChemistry Fuzhoun

The studies of no chemisorption on TiO2(110) surface are the base of research to no decomposed to N2O on TiO2 surface. In this paper, 12 kinds of possible models of no adsorbed on TiO2 perfect and defect surface were calculated by use of ab initio cluster method. We carried out optimization of the geometry, calculation of the chemisorption energy and analysis of the Mulliken population to those adsorption models. According to the calculation results, it can be got that the adsorbed decomposition of no on defect surface is more advantageous and M6 and M12 are the important models to no chemisorption and decomposition on TiO2 surface.

关键词： TiO2 (110) surface no chemisorption quantum chemistry

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Magnesium lithospermate B ameliorates microcirculation perfusion in rats by promoting vascular no production via activating the PI3K/AKT pathway

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Acta Pharmacologica Sinica 2019年第8期40卷 1010-1018页

作者： Ying-luo Liu Xiao-yu Zhou Li-jiang Xuan State Key Laboratory of Drug Research Shanghai Institute of Materia Medica School of Pharmacy University of Chinese Academy of Sciences

Microcirculation morphologically refers to the blood flow in vessels of less than 150 pm in diameter,including arterioles,capillaries and venules,which provides nutrients and removes metabolic byproducts within *** dysfunction is involved in the pathological progress of many diseases,such as obesity,hypertension,and insulin *** this study we investigated the effects of magnesium lithospermate B(MLB), an active compound of the traditional Chinese medicine Slavia miltiorrhiza,on the microcirculation dysfunction in rats and the underlying molecular *** effects of MLB on microcirculation were assessed in vivo by measuring the hindlimb blood perfusion in dextran-induced microcirculation dysfunction rats and mesentery blood flow in anesthetized *** demonstrated that administration of MLB restored the impaired rat hindlimb blood flow and promoted the mesenteric micoperfusion in *** further revealed in these two animal models that MLB treatment significantly increased the production of total nitrite in vascular tissues(mesentery,aorta,and heart),which was confirmed in human microvascular endothelial cells(HMEC-1)treated with MLB in ***,we showed that MLB treatment significantly increased the phosphorylation of endothelium nitric oxide synthase(enoS)via inducing AKT phosphorylation in vivo and in ***-administration of the enoS inhibitor L-NAME(20 mg/kg)abolished the protective effects of MLB against dextran-induced microcirculation dysfunction in rats,whereas pretreatment with PI3K inhibitor LY294002(10 μM)prevented enoS activation in MLBtreated HMEC-1 *** results suggest that MLB can restore the microcirculation dysfunction via activating enoS,and in turn enhancing the vascular nitric oxide production,which is medicated by MLB-caused activation of the PI3K/AKT pathway.

关键词： traditional Chinese medicine Salvia miltiorrhiza magnesium lithospermate B microcirculation dysfunction no endothelium nitric oxide synthase PI3K/AKT pathway

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