Mulberry leaf flavonoids protect against glucotoxicity-induced INS-1 cell apoptosis

作     者：Wang Ying Li Ming Yu Xuesong He Sheng Wu Xinrong Wang Yan 

作者机构：School of Chemistry Engineering Guangdong Pharmaceutical University School of Biosciences and Biopharmaceutics Guangdong Pharmaceutical University Department of Pharmacy General Hospital of Guangzhou Military Command of People's Liberation Army School of Traditional Chinese Medicine Guangdong Pharmaceutical University 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2019年第39卷第2期

页      面：153-159页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 

基　　金：Supported by the Natural Science Foundation of China(No.81673608) Guangdong Science and Technology Project(No.2015A020211034) Guangzhou Science and Technology Project(No.201707010155):Study on Targeting Delivery to Liver by Sinomenine-Encapsulating Exosomes of Human Hepatic Stem Cells and Molecular Mechanism 

主　　题：Morus Plant leaves In suli no ma Flavon oids Toxicity Apoptosis 

摘      要：OBJECTIVE:To investigate the effect of mulberry leaf flavonoids (MLF) on apoptosis of pancreatic cells induced by high ***:Long exposure to high glucose induces apoptosis of pancreaticβcells, which can lead to diabetes. In this study, we used the rat insulinoma cell line, INS-1. High glucose (33.3 mM) was used to establish a glucotoxicity model. The MTT assay was used to evaluate the MLF effect on cell ***-1 cells were treated with various concentra-tions of MLF (125, 250 and 500 mg/L) for 24 h, and then stimulated with 5.5 or 33.3 mM glucose for 48 ***, the cell supernatants were collected for enzyme-linked immunosorbent assay to determine the level of superoxide dismutase (SOD), catalase(CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), monocyte chemoattractant protein1 (MCP-1), tumor necrosis factor a (TNF-α) and interleukin 6 (IL-6). Western blotting was used to determine the expression of Bcl-2, Bax, caspase-3 and Caspase-9. Cell apoptosis was measured by Annexin V-FITC/propidium iodide double staining and flow ***:MLF (125-500 mg/L) improved cell viability. Furthermore, MLF (250 and 500 mg/L) inhibited apoptosis induced by high glucose. The anti-apoptosis effect of MLF was associated with increased SOD, CAT and GSH-Px expression, as well as reduced MDA levels in high-glucose-treated INS-1cells. Moreover, MLF upregulated Bcl-2 expression,downregulated Bax expression, and reduced the expression of caspase-3 and Caspase-9. Finally, MLF decreased the secretion of inflammatory cytokines and insulin in high-glucose-induced INS-1 ***:MLF is a potential therapeutic agent for preventing diabetes and related disorders.