Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor

作     者：HAO Jian DING Xiu-li YANG Xue WU Xiong-zhi HAO Jian;DING Xiu-li;YANG Xue;WU Xiong-zhi

作者机构：Zhong-Shan-Men In-Patient DepartmentTianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin(300060)China Clincal Center of Tumor TherapyThe Fourth Central Hospital Affiliated to Nankai UniversityTianjin(300140)China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2020年第26卷第4期

页      面：270-276页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the National Science Foundation of China(No.81173376) New Century Excellent Talent(No.NCET-11-1068) 

主　　题：Prunella vulgaris polysaccharide carci no ma-associated fibroblasts basic fibroblast growth factor SKBr-3 Chinese medicine 

摘      要：Objective:To study the effects of Prunella vulgaris polysaccharide(PVP)on human breast carcinoma-associated fibroblasts(CAFs).Method:Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)?2H?tetrazolium(MTS)*** healing experiment and transwell migration assay were used to investigate the anti-migration *** cytometry was applied to detect cell apoptosis and cell cycle *** transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor(bFGF)in *** SKBr-3 with CAFs conditioned medium(CAFs-CM)to evaluate the indirect function on the proliferation of breast cancer SKBr-3 ***:PVP inhibited the viability of CAFs by inducing apoptosis(P0.01)and arresting cell cycle(P0.01).It also inhibited the migration of CAFs(P0.01).bFGF promoted CAFs proliferation(P0.01)and migration(P0.01),protected CAFs from apoptosis(P0.05)and reduced Go phase to 49.06%(P0.01).However,these effects of bFGF on CAFs could be abrogated by *** SKBr-3 with CAFs-CM,PVP could inhibit the viability of breast cancer SKBr-3 cells ***,PVP reduced the mRNA expression(P0.01)and protein secretion of bFGF(P0.01)in ***:PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expressi on,thus inhibit!ng the growth of breast can cer SKBr-3 cells in directly.