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prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor

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Chinese Journal of Integrative Medicine 2020年第4期26卷 270-276页

作者： HAO Jian DING Xiu-li YANG Xue WU Xiong-zhi Zhong-Shan-Men In-Patient Department Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin(300060)China Clincal Center of Tumor Therapy The Fourth Central Hospital Affiliated to Nankai UniversityTianjin(300140)China

Objective:To study the effects of prunella vulgaris polysaccharide(PVP)on human breast carcinoma-associated fibroblasts(CAFs).Method:Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)?2H?tetrazolium(MTS)*** healing experiment and transwell migration assay were used to investigate the anti-migration *** cytometry was applied to detect cell apoptosis and cell cycle *** transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor(bFGF)in *** SKBr-3 with CAFs conditioned medium(CAFs-CM)to evaluate the indirect function on the proliferation of breast cancer SKBr-3 ***:PVP inhibited the viability of CAFs by inducing apoptosis(P<0.01)and arresting cell cycle(P<0.01).It also inhibited the migration of CAFs(P<0.01).bFGF promoted CAFs proliferation(P<0.01)and migration(P<0.01),protected CAFs from apoptosis(P<0.05)and reduced Go phase to 49.06%(P<0.01).However,these effects of bFGF on CAFs could be abrogated by *** SKBr-3 with CAFs-CM,PVP could inhibit the viability of breast cancer SKBr-3 cells ***,PVP reduced the mRNA expression(P<0.01)and protein secretion of bFGF(P<0.01)in ***:PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expressi on,thus inhibit!ng the growth of breast can cer SKBr-3 cells in directly.

关键词： prunella vulgaris polysaccharide carci no ma-associated fibroblasts basic fibroblast growth factor SKBr-3 Chinese medicine

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The Effect of <i>prunella</i>on Anti-Inflammatory Activity in RAW264.7 Mouse Macrophage Cells

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Food and Nutrition Sciences 2012年第9期3卷 1290-1295页

作者： Meehye Kim Food Safety Evaluation Department Korea Food and Drug Administration Chungcheongbuk-do Korea

The extracts of prunella vulgaris L. (Labiatae), a popular Western and Chinese herbal medicine, was shown to have anti-inflammatory properties, which might be due to partially, their rosmarinic acid content. Inhition of prostaglandine E2 (PGE2) production in lipopolysaccharide (LPS) stimulated RAW264.7 mouse macrophage cells was assessed with an enzyme immunoassay (EIA) following 8-hour treatments with prunella vulgaris extracts or fractions. Results showed that 95% ethanol extracts from P. vulgaris significantly inhibited PGE2 production. In further studies, fraction 2 from the 95% ethanol extract of P. vulgaris significantly reduced PGE2 production at 66 μg/ml (72% reduction). Cytotoxic-ity did not play a role in the noted reduction of PGE2 seen in either the extracts or fractions from P. vulgaris. High performance liquid chromatography analysis showed that there was 1.4 mM rosmarinic acid in 95% ethanol prunella extract (201 mg/ml crude extract). Our results suggest that rosmarinic acid may contribute toward the anti-inflammatory activity of prunella in a dose-response manner. prunella might have a potential to be used as a functional food for anti-inflammatory activity.

关键词： prunella Vulgaris Rosmarinic Acid Ursolic Acid Anti-Inflammatory Activity Cytotoxicity

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Orally administered extract from prunella vulgaris attenuates spontaneous colitis in mdr1a^(-/-) mice

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2015年第4期6卷 223-237页

作者： Kelley MK Haarberg Meghan J Wymore Brand Anne-Marie C Overstreet Catherine C Hauck Patricia A Murphy Jesse M Hostetter Amanda E Ramer-Tait Michael J Wannemuehler Department of Veterinary Microbiology and Preventive Medicine College of Veterinary Medicine Iowa State University Department of Food Science and Human Nutrition Iowa State University Department of Veterinary Pathology College of Veterinary Medicine Iowa State University Department of Food Science and Technology University of Nebraska-Lincoln

AIM: To investigate the ability of a prunella vulgaris(P. vulgaris) ethanolic extract to attenuate spontaneous typhlocolitis in mdr1a-/- mice. METHODS: Vehicle(5% ethanol) or P. vulgaris ethanolic extract(2.4 mg/d) were administered daily by oral gavage to mdr1a-/- or wild type FVBWT mice from 6 wk of age up to 20 wk of age. Clinical signs of disease were noted by monitoring weight loss. Mice experiencingweight loss in excess of 15% were removed from the study. At the time mice were removed from the study, blood and colon tissue were collected for analyses that included histological evaluation of lesions, inflammatory cytokine levels, and myeloperoxidase activity. RESULTS: Administration of P. vulgaris extracts to mdr1a-/- mice delayed onset of colitis and reduced severity of mucosal inflammation when compared to vehicle-treated mdr1a-/- mice. Oral administration of the P. vulgaris extract resulted in reduced(P < 0.05) serum levels of IL-10(4.6 ± 2 vs 19.4 ± 4), CXCL9(1319.0 ± 277 vs 3901.0 ± 858), and TNFα(9.9 ± 3 vs 14.8 ± 1) as well as reduced gene expression by more than two-fold for Ccl2, Ccl20, Cxcl1, Cxcl9, IL-1 α, Mmp10, VCAM-1, ICAM, IL-2, and TNFα in the colonic mucosa of mdr1a-/- mice compared to vehicle-treated mdr1a-/-mice. Histologically, several microscopic parameters were reduced(P < 0.05) in P. vulgaris-treated mdr1a-/-mice, as was myeloperoxidase activity in the colon(2.49 ± 0.16 vs 3.36 ± 0.06, P < 0.05). The numbers of CD4+ T cells(2031.9 ± 412.1 vs 5054.5 ± 809.5) and germinal center B cells(2749.6 ± 473.7 vs 4934.0 ± 645.9) observed in the cecal tonsils of P. vulgaris-treated mdr1a-/- were significantly reduced(P < 0.05) from vehicle-treated mdr1a-/- mice. Vehicle-treated mdr1a-/- mice were found to produce serum antibodies to antigens derived from members of the intestinal microbiota, indicative of severe colitis and a loss of adaptive tolerance to the members of the microbiota. These serum antibodies were greatly reduced or absent in P. vulga

关键词： prunella vulgaris Spontaneous colitis Inflammatory

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夏枯草诱导人甲状腺乳头状癌细胞K1增殖和凋亡的影响及其作用机制

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现代生物医学进展 2017年第13期17卷 2401-2406页

作者：熊燚赵敏谭剑斌吴仕漩杨哲张文娟杨杏芬暨南大学医学院广东广州510632 广东省疾病预防控制中心广东广州511430 深圳市龙岗区第二人民医院广东深圳518114 中山大学公共卫生学院广东广州510080

目的:探索夏枯草对人甲状腺乳头状癌细胞(K1)增殖和诱导K1细胞凋亡的影响及其可能的作用机制。方法:采用MTT比色法测定2~200 mg/mL夏枯草作用24 h对K1细胞增殖的影响;采用Hoechst染色法和流式细胞术(Annexin V-FITC/PI联合标记)观察0.3... 详细信息

目的:探索夏枯草对人甲状腺乳头状癌细胞(K1)增殖和诱导K1细胞凋亡的影响及其可能的作用机制。方法:采用MTT比色法测定2~200 mg/mL夏枯草作用24 h对K1细胞增殖的影响;采用Hoechst染色法和流式细胞术(Annexin V-FITC/PI联合标记)观察0.3、0.6、1.2、2.4、4.8 mg/mL夏枯草作用24 h K1细胞凋亡的影响;采用蛋白质免疫印迹法(Western blotting)测定1.2、2.4、4.8 mg/mL夏枯草作用24h对K1细胞凋亡相关蛋白表达的影响。结果:在2~200 mg/mL的浓度范围内夏枯草作用24 h对K1细胞增殖有明显抑制作用(P<0.05),IC50值为2.427 mg/mL。流式细胞术检测结果显示夏枯草可诱导K1细胞凋亡,2.4、4.8 mg/mL组K1细胞总凋亡率分别为(11.35±0.92)%、(44.57±3.07)%,与对照组相比明显升高(P<0.05);与对照组相比,1.2、2.4、4.8 mg/mL夏枯草作用24 h胞浆内凋亡蛋白Bax、细胞色素C(Cyto C)、Caspase-9、活化的Caspase-3(Cleaved Caspase-3)表达增多。结论:夏枯草能抑制人甲状腺癌K1细胞增殖并诱导其凋亡,其机制可能与活化线粒体凋亡通路有关。

关键词：夏枯草人甲状腺乳头状癌细胞凋亡线粒体通路 prunella vulgaris L.

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贺兰山岩鹨

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野生动物 1989年第2期10卷 34-34页

作者：刘乃发张迈梅兰州大学生物系

贺兰山岩鹨prunella Kosiowi(Przewalski)自1887年Przewalskii命名以来国内一直没有采到标本,1986年冬笔者在宁夏中卫沙坡头考查过程中采到该鸟标本。报导如下,以再建国内地模标本。形态额、头顶、后颈、背至尾上覆羽棕沙色,各羽均具褐... 详细信息

贺兰山岩鹨prunella Kosiowi(Przewalski)自1887年Przewalskii命名以来国内一直没有采到标本,1986年冬笔者在宁夏中卫沙坡头考查过程中采到该鸟标本。报导如下,以再建国内地模标本。形态额、头顶、后颈、背至尾上覆羽棕沙色,各羽均具褐色纵纹。尾羽褐色,中央一对尾羽具棕色羽缘。眼先灰白色,无眉纹,两颊、耳羽棕色,沾沙色。颏、喉淡褐,羽端沾白色。前颈、上胸羽基黑褐色,各羽端淡棕色,因而呈黑褐鳞斑状,下胸污白;腹,尾下覆羽棕白色。两胁淡棕色,各羽微见褐色纵纹。翅及翅上覆羽褐色,初级飞羽,次级飞羽具棕色羽缘。

关键词：贺兰山岩鹨淡棕色地模标本纵纹三级飞羽次级飞羽初级飞羽 prunella 嘴峰耳羽

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耤河下游地区唇形科药用植物调查研究初报

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天水师范学院学报 1991年第1期11卷 11-15页

作者：张德华王安民

一、地理环境横穿天水市区的(耒昔)河,是渭河的重要支流之一.它发源于甘谷县境内的石鼓山麓,蜿蜒东流,在北道区的河口入渭,全长近百公里.古城天水,就座落在(耒昔)河下游狭长的小型冲积平原上.

关键词：唇形科药用植物北道区重要支流百里香甘谷县 prunella 小坚果水棘针性味功能传粉昆虫

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