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niosome-encapsulated auraptene reduced the mRNA expression of VEGF-A and PDGFs genes in human retinaderived RPE cell line

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1028-1035页

作者： Akram Vahidi Teymoor Khosravi Farzad Dastaviz Mehdi Sheikh Arabi Ayyoob Khosravi Morteza Oladnabi Student Research Committee Golestan University of Medical SciencesGorgan ***Iran Department of Medical Nanotechnology Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Stem Cell Research Center Golestan University of Medical SciencesGorgan ***Iran Department of Molecular Medicine Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Gorgan Congenital Malformations Research Center Golestan University of Medical SciencesGorgan ***Iran

AIM:To evaluate the effect of auraptene(AUR)treatment in forms of free and encapsulated in niosome nanoparticles by investigating the mRNA expression level of vascular endothelium growth factor(VEGF)-A and platelet-derived growth factors(PDGFs)in human retinal pigment epithelium(RPE)cell ***:niosome nanocarriers were produced using two surfactants Span 60 and Tween *** cell line was treated with both free AUR and *** dosage of treatments was calculated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)*** of VEGF-A and PDGF-A,PDGF-B,PDGF-C,PDGF-D genes was measured after total RNA extraction and cDNA synthesis,using real-time polymerase chain reaction(RT-PCR).RESULTS:The highest entrapment efficiency(EE)was achieved by Span 60:cholesterol(1:1)with 64.3%.The half maximal inhibitory concentration(IC50)of free and niosome-encapsulated AUR were 38.5 and 27.78µg/mL,*** study revealed that niosomal AUR had more gradual delivery to the ***-PCR results showed reduced expression levels of VEGF-A,PDGF-A,PDGF-B,PDGF-C,and PDGF-D after treatment with both free and niosomal ***:Niosomal formulation of Span 60:cholesterol(1:1)is an effective drug delivery approach to transfer AUR to RPE ***-A,PDGF-A,PDGF-B,PDGF-C,and PDGF-D are four angiogenic factors,inhibiting which by niosomal AUR may be effective in age-related macular degeneration.

关键词： auraptene niosome age-related macular degeneration angiogenesis nanocarrier

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Impact of umbelliprenin-containing niosome nanoparticles on VEGF-A and CTGF genes expression in retinal pigment epithelium cells

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International Journal of Ophthalmology(English edition) 2024年第1期17卷 7-15页

作者： Farzad Dastaviz Akram Vahidi Teymoor Khosravi Ayyoob Khosravi Mehdi Sheikh Arabi Abouzar Bagheri Mohsen Rashidi Morteza Oladnabi Stem Cell Research Center Golestan University of Medical SciencesGorgan ***Iran Department of Medical Genetics School of Advanced Technologies in MedicineGolestan University of Medical SciencesGorgan ***Iran Student Research Committee Golestan University of Medical SciencesGorgan ***Iran Department of Molecular Medicine Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Department of Medical Nanotechnology Faculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Department of Clinical Biochemistry and Genetics Faculty of MedicineMazandaran University of Medical SciencesSari ***Iran Department Pharmacology Faculty of MedicineMazandaran University of Medical SciencesSari ***Iran Gorgan Congenital Malformations Research Center Golestan University of Medical SciencesGorgan ***Iran

AIM:To investigate the impact of niosome nanoparticles carrying umbelliprenin(UMB),an anti-angiogenic and anti-inflammatory plant compound,on the expression of vascular endothelial growth factor(VEGF-A)and connective tissue growth factor(CTGF)genes in a human retinal pigment epithelium(RPE)-like retina-derived cell ***:UMB-containing niosomes were created,optimized,and ***-like cells were treated with free UMB and UMB-containing *** IC_(50)values of the treatments were determined using an MTT *** expression of VEGF-A and CTGF was evaluated using real-time polymerase chain reaction after RNA extraction and cDNA ***’characteristics,including drug entrapment efficiency,size,dispersion index,and zeta potential were *** UMB had an IC_(50)of 96.2μg/mL,while UMB-containing niosomes had an IC_(50)of 25μg/***:Treatment with UMB-containing niosomes and free UMB resulted in a significant reduction in VEGF-A expression compared to control cells(P=0.001).Additionally,UMB-containing niosomes demonstrated a significant reduction in CTGF expression compared to control cells(P=0.05).However,there was no significant reduction in the expression of both genes in cells treated with free ***:Both free UMB and niosome-encapsulated UMB inhibits VEGF-A and CTGF genes ***,the latter demonstrates significantly greater efficacy,potentially due to the lower UMB dosage and gradual *** findings have implications for anti-angiogenesis therapeutic approaches targeting age-related macular degeneration.

关键词： umbelliprenin niosome age-related macular degeneration vascular endothelial growth factor-A connective tissue growth factor

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Preparation of Hollow Polypyrrole Nanospheres in niosome System

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Chemical Research in Chinese Universities 2013年第3期29卷 607-610页

作者： WANG Yuan-you LIU Tian-qing XU Wei-wei School of Chemistry and Chemical Engineering Yangzhou University Yangzhou 225002 P. R. China Department of Chemical Engineering Yangzhou Polytechnic Institute Yangzhou 225127 P. R. China

Hollow polypyrrole nanospheres were successfully prepared in a Span80/PEG400/H20（PEG=polyethylene glycol） niosome system. The formation and morphology of the nanospheres vary with the concentrations of pyrrole monomer, salt and Span80. The shell thickness of the nanospheres increases gradually with monomer content. NaC1 can promote the formation of the nanospheres. The formation mechanism of the nanospheres was discussed.

关键词： Hollow nanosphere niosome Polypyrrole Preparation

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Tumor-targeted niosome as novel carrier for intravenous administration of tocotrienol

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 79-80页

作者： Ju-Yen Fu Doryn Meam Yee Tan Hui Meng Er Yu Sui Chen Kalanithi Nesaretnam Malaysian Palm Oil Board Bandar Baru BangiSelangor 43000Malaysia International Medical University Bukit JalilKuala Lumpur 57000Malaysia

Tocotrienol(T3),the unsaturated form of vitamin E,has gained global attention due to its potent anticancer effects against a wide range of *** abundantly in palm oil[1],T3was reported to exhibit their anti-cancer properties via various pathways including anti-proliferative,anti-angiogenesis,antiinflammatory,apoptosis and improvement of immunological function[2].However,the lack of tumor specificity and rapid body clearance have restricted the clinical applications of T3[3].

关键词： Tocotrienol niosome Tumour-targeted Breast cancer

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Tioxolone niosomes exert antileishmanial effects on Leishmania tropica by promoting promastigote apoptosis and immunomodulation

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Asian Pacific Journal of Tropical Medicine 2019年第8期12卷 365-374页

作者： Maryam Hakimi Parizi Iraj Sharifi Saeedeh Farajzadeh Abbas Pardakhty Mohammad Hossein Daie Parizi Hamid Sharifi Ali Reza Keyhani Mahshid Mostafavi Mehdi Bamorovat Ahmad Khosravi Daryoush Ghaffari Leishmaniasis Research Center Kerman University of Medical SciencesKermanIran Department of Pediatric dermatology Kerman University of Medical SciencesKermanIran Pharmaceutics Research Center Neuropharmacology InstituteKerman University of Medical SciencesKermanIran Department of Pediatrics Kerman University of Medical SciencesKermanIran HIV/STI Surveillance Research Center and WHO Collaborating Center for HIV SurveillanceInstitute for Futures Studies in HealthKerman University of Medical SciencesKermanIran Research Center of Tropical and Infectious Diseases Kerman University of Medical SciencesKermanIran

Objective: To explore the antileishmanial effect of tioxolone and its niosomal form against Leishmania tropica. Methods: Tioxolone niosomes were prepared by the hydration method and were evaluated for morphology, size, release study, and encapsulation efficiency. The cytotoxicity of tioxolone and its niosomal form was measured by MTT assay, leishmanicidal activity against promastigote and amastigote by MTT assay, apoptosis by flow cytometry, IL-12, IL-10 and metacaspase gene expression levels by q-PCR. Results: Span/Tween 40 and Span/Tween 60 niosomes had good physical stability as depicted in their size distribution curves and high encapsulation efficiency(>99%). The release profile of the entrapped compounds showed Fickian’s model of tioxolone delivery based on diffusion through lipid bilayers. With the IC50 value for amastigote as(24.5±2.1) μg/mL and selectivity index as 10.5, the Span/Tween 60 niosome(NT2) had a superior effect to other drugs. The CC50 value and IC50 of promastigote value for NT2 were(257.5±24.5) μg/mL and(164.8±20.6) μg/mL, respectively. The flow cytometric analysis showed that tioxolone and niosomal forms induced apoptosis of Leishmania tropica promastigotes in a dose-dependent manner. NT2 increased the expression level of IL-12 and metacaspase genes and decreased the expression level of the IL-10 ***: niosomes of tioxolone play an immunomodulatory role in increasing Th1 cytokine profile and inhibiting the Th2 cytokine profile. It could be used for treatment of anthroponotic cutaneous leishmaniasis.

关键词： niosome Tioxolone Leishmania tropica Apoptosis

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非离子表面活性剂囊泡作为药物载体的进展

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中国医药工业杂志 1998年第5期29卷 235-240页

作者：王大林盛坤贤上海医药工业研究院

综述了８０年代以来非离子表面活性剂囊泡（ｎｉｏｓｏｍｅ）的制备、结构、性能、特点、毒性、稳定性和体内行为，以及影响其大小、包裹率、释药速率的因素，并介绍了它在药剂上的应用及发展方向。

关键词：药物载体盘状体 niosome

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