Impact of umbelliprenin-containing niosome nanoparticles on VEGF-A and CTGF genes expression in retinal pigment epithelium cells

作     者：Farzad Dastaviz Akram Vahidi Teymoor Khosravi Ayyoob Khosravi Mehdi Sheikh Arabi Abouzar Bagheri Mohsen Rashidi Morteza Oladnabi 

作者机构：Stem Cell Research CenterGolestan University of Medical SciencesGorgan ***Iran Department of Medical GeneticsSchool of Advanced Technologies in MedicineGolestan University of Medical SciencesGorgan ***Iran Student Research CommitteeGolestan University of Medical SciencesGorgan ***Iran Department of Molecular MedicineFaculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Department of Medical NanotechnologyFaculty of Advanced Medical TechnologiesGolestan University of Medical SciencesGorgan ***Iran Department of Clinical Biochemistry and GeneticsFaculty of MedicineMazandaran University of Medical SciencesSari ***Iran Department PharmacologyFaculty of MedicineMazandaran University of Medical SciencesSari ***Iran Gorgan Congenital Malformations Research CenterGolestan University of Medical SciencesGorgan ***Iran 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2024年第17卷第1期

页      面：7-15页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by Stem Cell Research Center of Golestan University of Medical Sciences(No.110480) 

主　　题：umbelliprenin niosome age-related macular degeneration vascular endothelial growth factor-A connective tissue growth factor 

摘      要：AIM:To investigate the impact of niosome nanoparticles carrying umbelliprenin(UMB),an anti-angiogenic and anti-inflammatory plant compound,on the expression of vascular endothelial growth factor(VEGF-A)and connective tissue growth factor(CTGF)genes in a human retinal pigment epithelium(RPE)-like retina-derived cell ***:UMB-containing niosomes were created,optimized,and ***-like cells were treated with free UMB and UMB-containing *** IC_(50)values of the treatments were determined using an MTT *** expression of VEGF-A and CTGF was evaluated using real-time polymerase chain reaction after RNA extraction and cDNA ***’characteristics,including drug entrapment efficiency,size,dispersion index,and zeta potential were *** UMB had an IC_(50)of 96.2μg/mL,while UMB-containing niosomes had an IC_(50)of 25μg/***:Treatment with UMB-containing niosomes and free UMB resulted in a significant reduction in VEGF-A expression compared to control cells(P=0.001).Additionally,UMB-containing niosomes demonstrated a significant reduction in CTGF expression compared to control cells(P=0.05).However,there was no significant reduction in the expression of both genes in cells treated with free ***:Both free UMB and niosome-encapsulated UMB inhibits VEGF-A and CTGF genes ***,the latter demonstrates significantly greater efficacy,potentially due to the lower UMB dosage and gradual *** findings have implications for anti-angiogenesis therapeutic approaches targeting age-related macular degeneration.