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Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for natural pigment Production

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Chinese Journal of Chemical Engineering 2010年第6期18卷 1013-1017页

作者： LIU Jiefeng REN Yiran YAO Shanjing Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China Department of Biotechnology and Food Engineering Maoming College Maoming 525000 China

In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule's membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

关键词： microencapsulation Monascus purpureus natural pigment polyelectrolyte complex NaCS-PDMDAAC capsule cell immobilization

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Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering

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Synthetic and Systems Biotechnology 2023年第1期8卷 54-60页

作者： Lijuan Zhang Xue Liu Jiawei Li Yonghui Meng Guang-Rong Zhao Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityYaguan Road 135Jinnan DistrictTianjin300350China Georgia Tech Shenzhen Institute Tianjin UniversityDashi 1st RdNanshan DistrictShenzhen518055China

Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food *** relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based *** bioproduction therefore represents an attractive ***,we present the construction of a Saccharomyces cerevisiae strain for betanin *** minimizing metabolic crosstalk,screening and modifying biosynthetic enzymes,enhancing pathway flux and optimizing fermentation conditions,a final titer of betanin of 28.7 mg/L was achieved from glucose at 25℃ in baffled shake-flask,which is the highest reported titer produced by yeast to our *** work provides a promising step towards developing synthetic yeast cell factories for de novo biosynthesis of value-added betanin and other betacyanins.

关键词： natural pigment Betanin Saccharomyces cerevisiae Synthetic biology Metabolic engineering

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Biotechnological Potential of Carotenoids Produced by Extremophilic Microorganisms and Application Prospects for the Cosmetics Industry

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Advances in Microbiology 2020年第8期10卷 397-410页

作者： Tayane de Cássia Dias Mendes-Silva Rosileide Fontenele da Silva Andrade Marcio Akio Ootani Paulo Vitor Dias Mendes Rafael Artur de Queiroz Cavalcanti de Sá Milena Roberta Freire da Silva Karolayne Silva Souza Maria Tereza dos Santos Correia Márcia Vanusa da Silva Maria Betânia Melo de Oliveira Department of Biochemistry Federal University of Pernambuco Recife Brazil National Program Postdoctoral CAPES (PNPD) Catholic University of Pernambuco Recife Brazil Bioprocess Laboratory Center of Strategic Technologies Northeast Recife Brazil Design Department Federal University of Pernambuco Recife Brazil Nucleus of Bioprospecting and Conservation of the Caatinga Semiarid National Institute/Ministry of Science Technology Innovations and Communications Campina Grande Brazil

Carotenoids are isoprenoid pigments used in food, chemical, textile, pharmaceutical, and cosmetic industries. They act not only as dyes and provitamins A but also have antioxidants, photoprotective, antimicrobial properties, among others. This class of pigment can be obtained traditionally by plants or chemical synthesis, but they have some disadvantages. In recent years, search for alternative sources has been an important strategy for the carotenoid industries. Microbial synthesis is an alternative that has shown good yields, speed, and reduced production costs. Hostile environments, such as the Caatinga domain, represent an interesting source of microorganisms that produce biomolecules, especially carotenoids, because of oxidative stress caused by sunlight. Thus, this region has been attracting the attention of the scientific community and industry for the use of these organisms in the production of carotenoids and applications in cosmetic products;since these compounds have interesting antioxidant and photoprotective properties. In this review, general characteristics of carotenoids, sources of production, industrial applicability, and commercialization will be discussed, as well as perspectives on the production of carotenoids from microorganisms isolated from the Caatinga and their application in anti-UV products.

关键词： natural pigment Microbial Production Hostile Environments Antioxidant Photoprotection

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耐温型天然色素大豆油基可食性油墨的制备研究

耐温型天然色素大豆油基可食性油墨的制备研究

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2013中国食品包装学术会议

作者：刘壮刘旗杨柳王锋利王冬梅张本龙哈尔滨商业大学轻工学院

绿色、可持续发展已成为当今科技的主题,天然环保油墨的研究是印刷领域凸显该主题的重要课题。然而,传统天然颜料大豆油基环保型油墨存在耐温性差的缺点。本文打破了传统天然颜料大豆油基油墨不能用于高温、加热食品及包装印刷的局限。... 详细信息

绿色、可持续发展已成为当今科技的主题,天然环保油墨的研究是印刷领域凸显该主题的重要课题。然而,传统天然颜料大豆油基环保型油墨存在耐温性差的缺点。本文打破了传统天然颜料大豆油基油墨不能用于高温、加热食品及包装印刷的局限。研制实用型耐温天然颜料大豆油基环保油墨具有重要现实意义。本文引入有机硅,并充分利用其Si-O键的键能121千卡/克分子,在高温下（或辐射照射）分子的化学键不断裂、不分解特性实现油墨耐高温性能。实验选用二甲基硅油、甲基三甲氧基硅烷、乙烯基三甲氧基硅烷和氨丙基三乙氧基硅烷试剂作为油墨的耐温试剂,以红色油墨为例,分析不同温度下不同组油墨的色密度、光谱反射率,探究油墨对高温的敏感性,以便提高其实用价值。实验表明,二甲基硅油与大豆油混合时,发生交联反应,生产白色絮状物,影响实验继续进行。随温度的升高,以甲基三甲氧基硅烷、乙烯基三甲氧基硅烷、氨丙基三乙氧基硅烷作为耐温试剂的油墨,初步表现耐温性:油墨的色强、灰度、色效率均在合理范围内变化,油墨的光谱反射曲线波动较小。氨丙基三乙氧基硅烷除可作为耐温试剂外,还具备调色剂的功用。

关键词： natural pigment Soybean oil ink Temperature resistance Silicone

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