Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering

作     者：Lijuan Zhang Xue Liu Jiawei Li Yonghui Meng Guang-Rong Zhao 

作者机构：Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education)School of Chemical Engineering and TechnologyTianjin UniversityYaguan Road 135Jinnan DistrictTianjin300350China Georgia Tech Shenzhen InstituteTianjin UniversityDashi 1st RdNanshan DistrictShenzhen518055China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2023年第8卷第1期

页      面：54-60页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 0836[工学-生物工程] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Research and Development Program in Key Areas of Guangdong Province China (2020B0303070002) 

主　　题：Natural pigment Betanin Saccharomyces cerevisiae Synthetic biology Metabolic engineering 

摘      要：Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food *** relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based *** bioproduction therefore represents an attractive ***,we present the construction of a Saccharomyces cerevisiae strain for betanin *** minimizing metabolic crosstalk,screening and modifying biosynthetic enzymes,enhancing pathway flux and optimizing fermentation conditions,a final titer of betanin of 28.7 mg/L was achieved from glucose at 25℃ in baffled shake-flask,which is the highest reported titer produced by yeast to our *** work provides a promising step towards developing synthetic yeast cell factories for de novo biosynthesis of value-added betanin and other betacyanins.