Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

作     者：LIU Jiefeng REN Yiran YAO Shanjing LIU Jiefeng;REN Yiran;YAO Shanjing

作者机构：Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China Department of Biotechnology and Food Engineering Maoming College Maoming 525000 China 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2010年第18卷第6期

页      面：1013-1017页

核心收录：

学科分类：082903[工学-林产化学加工工程] 08[工学] 0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0817[工学-化学工程与技术] 0829[工学-林业工程] 0703[理学-化学] 082201[工学-制浆造纸工程] 0836[工学-生物工程] 0822[工学-轻工技术与工程] 082203[工学-发酵工程] 083203[工学-农产品加工及贮藏工程] 

基　　金：Supported by the National Basic Research Program of China(2007CB707805) the National Natural Science Foundation of China(20876139) 

主　　题：microencapsulation Monascus purpureus natural pigment polyelectrolyte complex NaCS-PDMDAAC capsule cell immobilization 

摘      要：In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule s membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.