检索结果-南通市图书馆

mitochondrial dynamics caused by QoIs and SDHIs fungicides depended on FgDnm1 in Fusarium graminearum

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第2期22卷 481-494页

作者： KANG Jin-bo ZHANG Jie LIU Yin-kai SONG Ji-chang OU Jian-lin TAO Xian ZHOU Ming-guo DUAN Ya-bing College of Plant Protection Nanjing Agricultural UniversityNanjing 210095P.R.China

Fusarium head blight(FHB) caused by Fusarium graminearum is a devastating fungal disease on small grain cereal crops,because it reduces yield and quality and causes the mycotoxin contamination to the *** and dynamin-related proteins(DRPs) are large GTPase superfamily members,which are typically involved in the budding and division of vesicles in eukaryotic cells,but their roles in Fusarium *** ***,we found that FgDnm1,a DRP and homolog to Dnm1 in Saccharomyces cerevisiae,contributes to the normal fungal growth,sexual reproduction and sensitivity to *** addition,we found FgDnm1 co-localizes with mitochondria and is involved in toxisome formation and deoxynivalenol(DON) *** quinone outside inhibitors(QoIs) and succinate dehydrogenase inhibitors(SDHIs) cause fragmentated morphology of ***,the deletion of FgDnm1displays filamentous mitochondria and blocks the mitochondrial fragmentation induced by QoIs and *** together,our studies uncover the effect of mitochondrial dynamics in fungal normal growth and how such events link to fungicides sensitivity and toxisome ***,we concluded that altered mitochondrial morphology induced by QoIs and SDHIs depends on FgDnm1.

关键词： Fusarium graminearum FgDnm1 mitochondrial dynamics fungicides

维普期刊数据库

Human mesenchymal stem cells exhibit altered mitochondrial dynamics and poor survival in high glucose microenvironment

在线全文

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2023年第12期15卷 1093-1103页

作者： Ejlal Abu-El-Rub Fatimah Almahasneh Ramada R Khasawneh Ayman Alzu'bi Doaa Ghorab Rawan Almazari Huthaifa Magableh Ahmad Sanajleh Haitham Shlool Mohammad Mazari Noor S Bader Joud Al-Momani Department of Basic Medical Sciences Faculty of MedicineYarmouk UniversityIrbid 21163Jordan Department of Pathology Faculty of MedicineMansoura UniversityMansoura 35516Egypt Department of Basic Medical Sciences Yarmouk UniversityIrbid 21163Jordan

BACKGROUND Mesenchymal stem cells(MSCs)are a type of stem cells that possess relevant regenerative abilities and can be used to treat many chronic *** mellitus(DM)is a frequently diagnosed chronic disease characterized by hyperglycemia which initiates many multisystem complications in the *** patients can benefit from MSCs transplantation to curb down the pathological consequences associated with hyperglycemia persistence and restore the function of damaged *** therapeutic outcomes are found to last for short period of time and ultimately these regenerative cells are eradicated and died in DM disease *** To investigate the impact of high glucose or hyperglycemia on the cellular and molecular characteristics of *** Human adipose tissue-derived MSCs(hAD-MSCs)were seeded in low(5.6 mmol/L of glucose)and high glucose(25 mmol/L of glucose)for 7 ***,viability,mitochondrial dynamics,and apoptosis were deplored using specific *** blotting was performed to measure the protein expression of phosphatidylinositol 3-kinase(PI3K),TSC1,and mammalian target of rapamycin(mTOR)in these *** hAD-MSCs cultured in high glucose for 7 d demonstrated marked decrease in their viability,as shown by a significant increase in lactate dehydrogenase(Pmitochondrial ***3K protein expression significantly decreased in high glucose culture MSCs(Pmitochondrial complexes I,IV,and V were downregul

关键词： Mesenchymal stem cells High glucose mitochondrial dynamics Apoptosis Poor survival Phosphatidylinositol 3-kinase/mammalian target of rapamycin pathway

Hypoxia promotes pulmonary vascular remodeling via HIF-1α to regulate mitochondrial dynamics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2019年第12期16卷 855-871页

作者： Xi CHEN Jia-Mei YAO Xia FANG Cui ZHANG Yu-Shu YANG Cheng-Ping HU Qiong CHEN Guang-Wei ZHONG National Center for Clinical Medicine for Geriatric Diseases Xiangya HospitalCentral South UniversityChangshaChina Department of Respiratory Medicine Xiangya HospitalCentral South UniversityChangshaChina Department of Geriatrics Xiangya HospitalCentral South UniversityChangshaChina International Medical Center Xiangya HospitalCentral South UniversityChangshaChina

Background Increasing research suggests that mitochondrial defect plays a major role in pulmonary hypertension(PH) pathogenesis. mitochondrial dynamics and quality control have a central role in the maintenance of the cell proliferation and apoptosis balance. However, the molecular mechanism underlying of this balance is still unknown. Methods To clarify the biological effects of hypoxic air exposure and hypoxia-inducible factor-1α(HIF-1α) on pulmonary arterial smooth muscle cell(PASMC) and pulmonary arterial hypertension rats, the cells were cultured in a hypoxic chamber under oxygen concentrations. Cell viability, reactive oxygen species level, cell death, mitochondrial morphology, mitochondrial membrane potential, mitochondrial function and mitochondrial biosynthesis, as well as fission-and fusion-related proteins, were measured under hypoxic conditions. In addition, rats were maintained under hypoxic conditions, and the right ventricular systolic pressure, right ventricular hypertrophy index and right ventricular weight/body weight ratio were examined and recorded. Further, we assessed the role of HIF-1α in the development and progression of PH using HIF-1α gene knockdown using small interfering RNA transfection. Mdivi-1 treatment was performed before hypoxia to inhibit dynamin-related protein 1(Drp1). Results We found that HIF-1α expression was increased during hypoxia, which was crucial for hypoxia-induced mitochondrial dysfunction and hypoxia-stimulated PASMCs proliferation and apoptosis. We also found that targeting mitochondrial fission Drp1 by mitochondrial division inhibitor Mdivi-1 was effective in PH model rats. The results showed that mitochondrial dynamics were involved in the pulmonary vascular remodeling under hypoxia in vivo and in vitro. Furthermore, HIF-1α also modulated mitochondrial dynamics in pulmonary vascular remodeling under hypoxia through directly regulating the expression of Drp1. Conclusions In conclusion, our data suggests that abnorm

关键词： Dynamin-related protein 1 Hypoxia Hypoxia-inducible factor-1α mitochondrial dynamics Pulmonary vascular remodeling

维普期刊数据库

The essential functions of mitochondrial dynamics in immune cells

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第7期17卷 712-721页

作者： Jia-Huan Xie Yi-Yuan Li Jin Jin MOE Laboratory of Biosystem Homeostasis and Protection and Life Sciences Institute Zhejiang UniversityHangzhou 310058China Key Laboratory for Developmental Genes and Human Disease Ministry of EducationInstitute of Life SciencesJiangsu Province High-Tech Key Laboratory for Biomedical ResearchSoutheast UniversityNanjing 210096China Sir Run Run Shaw Hospital College of Medicine Zhejiang UniversityHangzhou 310016China

Mitochondria are highly mobile organelles due to fission,fusion,transport,and mitophagy,and these processes are known as mitochondrial *** dynamics play an important role in energy production,cell division,cell differentiation,and cell *** the past decade,numerous studies have revealed the importance of mitochondrial metabolism in immunity,and mitochondrial dynamics are essential for immune responses mediated by various cell *** this review,we mainly discuss the role of mitochondrial dynamics in activation,differentiation,cytokine production,and the activity of related pathways in immune cells,particularly T cells,B cells,and other cells involved in the innate immune response.

关键词： mitochondrial dynamics Metabolism T cell B cell Innate immune cell

Dual Oxidase Mutant Retards Mauthner-Cell Axon Regeneration at an Early Stage via Modulating mitochondrial dynamics in Zebrafish

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2020年第12期36卷 1500-1512页

作者： Lei-Qing Yang Min Chen Da-Long Ren Bing Hu Eye Center The First Affiliated Hospital of USTCHefei National Laboratory for Physical Sciences at the MicroscaleChinese Academy of Sciences Key Laboratory of Brain Function and DiseaseSchool of Life SciencesDivision of Biomedical SciencesUniversity of Science and Technology of ChinaHefei 230026China Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding College of Animal Science and TechnologyAnhui Agricultural UniversityHefei 230036China

Dual oxidase(duox)-deriyed reactive oxygen species(ROS)have been correlated with neuronal polarity,cerebellar development,and ***,there have not been many comprehensive studies of the effect of individual duox isoforms on central-axon regenerationin ***,we explored this question in zebrafish,an excellent model organism for central-axon regeneration *** our research,mutation of the duox gene with CRISPR/Cas9 significantly retarded the singleaxon regeneration of the zebrafish Mauthner cell in *** deep transcriptome sequencing,we found that the expression levels of related functional enzymes in mitochondria were down-regulated in duox mutant *** vivo imaging showed that duox mutants had significantly disrupted mitochondrial transport and redox state in single Mauthner-cell *** research data provide insights into how duox is involved in central-axon regeneration by changing mitochondrial transport.

关键词： duox Zebrafish Mauthner cell Axon regeneration mitochondrial dynamics

维普期刊数据库

Shenmai injection enhances cisplatin-induced apoptosis through regulation of Mfn2-dependent mitochondrial dynamics in lung adenocarcinoma A549/DDP cells

在线全文

学校读者我要写书评

暂无评论

Cancer Drug Resistance 2021年第4期4卷 1047-1060页

作者： Yushi Chen Ye Sun Qiuyu Zhao Chunying Liu Chun Wang College of Integrated Chinese and Western Medical Liaoning University of Traditional Chinese MedicineShenyang 110847LiaoningChina Basic Medical College Shenyang Medical CollegeShenyang 110034LiaoningChina Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications Liaoning University of Traditional Chinese MedicineShenyang 110847LiaoningChina

Aim:Chemoresistance is the biggest obstacle in cancer *** previous study demonstrated that Shenmai injection(SMI),a Chinese herbal medicine,enhanced the antitumor effect of cisplatin via glucose metabolism *** study aimed to further determine whether the SMI sensitizes the non-small cell lung cancer(NSCLC)cells to cisplatin through regulation mitochondrial ***:The Kaplan-Meier Plotter database was used to investigate the relationship between mRNA expression of mitofusin-2(Mfn2)and the survival analysis of NSCLC *** protein expression of Mfn2 in a lung adenocarcinoma tissue chip was detected by immunohistochemistry *** expression of Mfn2 and ATAD3A were compared between cisplatin-sensitive A549 and cisplatin-resistant A549/DDP ***,A549/DDP cells were co-treated with cisplatin and SMI to detect mitochondrial morphology by fluorescent staining,apoptosis-related protein expression with Western blotting,and mitochondrial membrane potential(ΔΨm)with flow cytometry ***:The mean survival time of the Mfn2^(low) group was significantly lower than that of the Mfn2^(high) group by Kaplan-Meier Plotter database analysis,and the Mfn2 protein expression level was lower in cancer tissues than in adjacent *** combination of SMI and cisplatin induced dynamic changes in A549/DDP cells,with increased mitochondrial fusion followed by upregulation of Mfn2 and downregulation of ATAD3A and reduced mitochondrial mass and ΔΨ***,SMI significantly enhanced cisplatin-induced A549/DDP apoptosis,upregulated Bax and the active subunit of caspase-3,and downregulated Bcl-2 expression,as shown via Hoechst staining and flow cytometry ***:Our findings suggest SMI enhances cisplatin-induced apoptosis through regulation of Mfn2-dependent mitochondrial dynamics in cisplatin-resistant lung adenocarcinoma cells.

关键词： SMI NSCLC cisplatin-resistant Mfn2 mitochondrial dynamics apoptosis

High glucose changes mitochondrial dynamics in cultured human glomerular endothelial cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国药理学与毒理学杂志 2019年第10期 927-928页

作者： YU Bai-xue SHI Yi Biomedical Research Center Zhongshan Hospital Fudan University

OBJECTIVE To investigate the effects of high glucose on mitochondrial dynamic changes in cultured human glomerular endothelial cells（HGECs）.METHODS HGECs were subjected to high glucose(44.4 mmol·L;) or mannitol for six-hour or *** were collected for real-time polymerase chain reactions. RESULTS After six hours, mRNA expression of mitochondrial fusion protein mitofusion（MFN）-2, but not MFN1, was increased in HGEC stimulated with high glucose. It suggests that exchanges of intramitochondrial material are enhanced. Message RNA expression of mitochondrial fission factor（MFF） was increased as well,indicating an increase in mitochondrial fragmentation,probably leading to mitophagy. Nevertheless, the enhanced mitochondrial dynamic changes were not observed after 24 h stimulation. Thus, the transient mitochondrial dynamic changes indicate an adaptive mechanism in HEGC challenging with high glucose. CONCLUSION High glucose transiently alters mitochondrial dynamic genes expression in HGEC. Further experiments will be designed to investigate underlying mechanisms in HGEC as well as in glomerular endothelial cells of diabetic nephropathy.

关键词： high glucose mitochondrial dynamics glomerular endothelial cells

Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biophysics Reports 2017年第Z2期3卷 64-72页

作者： Xingguo Liu Liang Yang Qi Long David Weaver Gy?rgy Hajnóczky Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences Department of Pathology Mito Care CenterAnatomy and Cell BiologyThomas Jefferson University

mitochondrial dynamics refers to the processes maintaining mitochondrial homeostasis, including mitochondrial fission, fusion, transport, biogenesis, and mitophagy. mitochondrial dynamics is essential for maintaining the metabolic function of mitochondria as well as their regulatory roles in cell *** this review, we summarize the recently developed imaging techniques for studying mitochondrial dynamics including: mitochondrial-targeted fluorescent proteins and dyes, live-cell imaging using photoactivation, photoswitching and cell fusion, mitochondrial transcription and replication imaging by in situ hybridization, and imaging mitochondrial dynamics by super-resolution microscopy. Moreover,we discuss examples of how to choose and combine proper fluorescent dyes and/or proteins.

关键词： Imaging mitochondrial dynamics Photoactivation Photoswitching Super-resolution microscope

mitochondrial dynamics controls antitumor innate immunity

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Mitochondrial dynamics controls antitumor innate immunity