Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells

作     者：Xingguo Liu Liang Yang Qi Long David Weaver Gy?rgy Hajnóczky 

作者机构：Key Laboratory of Regenerative BiologyGuangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences Department of PathologyMito Care CenterAnatomy and Cell BiologyThomas Jefferson University 

出 版 物：《Biophysics Reports》 (生物物理学报(英文))

年 卷 期：2017年第3卷第Z2期

页      面：64-72页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：financially supported by the "973" program (2013CB967403, 2012CB721105, and 2016YFA0100302) the "Frontier Science Key Research Program" of the Chinese Academy of Sciences (QYZDB-SSW-SMC001) the National Natural Science Foundation projects of China (31622037, 31271527, 81570520, 31601176, and 31601088) Guangzhou Science and Technology Program (2014Y2-00161) Guangzhou Health Care and Cooperative Innovation Major Project (201604020009) Guangdong Natural Science Foundation for Distinguished Young Scientists (S20120011368) Guangdong Province Science and Technology Innovation The Leading Talents Program (2015TX01R047) Guangdong Province Science and Technology Innovation Young Talents Program (2014TQ01R559) Guangdong Province Science and Technology Program (2015A020212031) the PhD Start-up Fund of Natural Science Foundation of Guangdong Province (2014A030310071) by NIH Grants (AA017773 and DK051526) to G.H 

主　　题：Imaging Mitochondrial dynamics Photoactivation Photoswitching Super-resolution microscope 

摘      要：Mitochondrial dynamics refers to the processes maintaining mitochondrial homeostasis, including mitochondrial fission, fusion, transport, biogenesis, and mitophagy. Mitochondrial dynamics is essential for maintaining the metabolic function of mitochondria as well as their regulatory roles in cell *** this review, we summarize the recently developed imaging techniques for studying mitochondrial dynamics including: mitochondrial-targeted fluorescent proteins and dyes, live-cell imaging using photoactivation, photoswitching and cell fusion, mitochondrial transcription and replication imaging by in situ hybridization, and imaging mitochondrial dynamics by super-resolution microscopy. Moreover,we discuss examples of how to choose and combine proper fluorescent dyes and/or proteins.