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Digital Chinese Medicine 2019年第4期2卷 219-226页

作者： ZHOU Fang-Liang HE Lan HE Dan WANG Xian-Wen SHI Hong-Jian HE Ying-Chun CAO De-Liang Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine Hunan University of Chinese MedicineChangshaHunan 410208China School of Medicine Hunan University of Chinese MedicineChangshaHunan 410208China Department of Medical Microbiology Immunology&Cell BiologySimmons Cancer InstituteSouthern Illinois University School of MedicineSpringfield 62702USA

Objective To assess the effects of Yi Qi Jie Du Decoction(YQJDD)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying *** NPC-SCs were collected in serum-free culture system and identified by the sphere formation *** effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 *** change of mitochondrial membrane potential(ΔΨm)in NPC-SCs following treatment with YQJDD was investigated by JC-1 *** levels of apoptosisassociated proteins were examined using western blot *** YQJDD significantly inhibited the proliferation of NPCSCs in dose-and time-dependent ***-1 staining revealed that YQJDD lowered theΔΨm of NPC-SCs in a timedependent *** treatment with YQJDD,the expression of cleaved caspase-3,-7 and-9,cleaved poly-ADP ribose polymerase,P21 and P53 were increased,while the expression of Survivin in NPC-SCs was ***,the expression of cleaved caspase-8 remained nearly *** YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.

关键词： Nasopharyngeal carcinoma(NPC) Yi Qi Jie Du Decoction(YQJDD) apoptosis Cancer stem cells mitochondrial apoptosis pathway

Fine particulate matters inducing reproductive impairment by causing cell cycle arrest and activating mitochondrial apoptosis pathway in male rats and spermatocyte cells

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Fine particulate matters inducing reproductive impairment by...

apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway

中国生理学会内分泌代谢、比较生理与应激生理学术会议

作者： Jianhui Liu Lihua Ren Jialiu Wei Jin Zhang Yannan Zhang Hejing Hu Junchao Duan Zhiwei Sun Xianqing Zhou Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

Fine particulate matters(PM2.5) have been shown to induce male reproductive toxicity,but the mechanism is *** explore the toxic mechanism of PM2.5,32 male Sprague-Dawley(SD)rats were treated to normal saline(control group) or PM2.5 with the doses of 1.8,5.4 and 16.2 mg/*** intratracheal instillation every 3 days for 30 *** 30 days,the rats were sacrificed for tissue *** vitro,GC-2 spd cells were treated to various concentrations(12.5,25,50 μg/mL) of PM2.5 for 24 *** results showed that PM2.5 obviously decreased sperm quality,increased sperm abnormality rate and damaged testicular tissue in male ***2.5 induced oxidative stress and DNA damage in ***,PM2.5 significantly increased the expression of BAX,cytochrome C,Caspase-9,Caspase-3 on the 30 th *** vitro,GC-2 cells treated with PM2.5 displayed mitochondria swelling and cristae ***2.5 induced S-phase cell cycle arrest and apoptosis in GC-2 spd *** results suggest that PM2.5 might induce apoptosis by up-regulating the expressions of mitochondrial apoptosis pathway factors through causing DNA damage and cell cycle arrest resulting from oxidative stress,and cause a decrease in the quantity and quality of sperms.

关键词： Fine particulate matters oxidative stress cell cycle arrest mitochondrial apoptosis pathway male reproduction

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Food Science and Human Wellness 2023年第5期12卷 1545-1556页

作者： Liuming Xie Mingyue Shen Rong Huang Xuan Liu Yue Yu Hanyu Lu Jianhua Xie State Key Laboratory of Food Science and Technology Nanchang UniversityNanchang 330047China

In this study,the antitumor properties and the possible molecular mechanisms of Cyclocarya paliurus polysaccharide(CP)and its phosphorylated derivative(P-CP)on CT-26 mouse colon carcinoma cells were *** found that CP had high inhibition ratio against CT-26 *** flow cytometry results found that CP treatment could cause the intracellular acidification,arrest the cell cycle in the S phase and increase reactive oxygen species ***,CP treatment triggered mitochondrial membrane potential depolarization and Ca^(2+)overloading,and broke down the balance of antioxidant system,Na^(+)/K^(+)-ATPase and Ca^(2+)-*** analysis found CP induced cell apoptosis through improving the activities of caspase-3 and caspase-9,and increasing the level of cytochrome ***,the comparative study of antitumor effect on CT-26 cells displayed that the phosphorylation enhanced antitumor activities of *** results suggest CP is a potential natural therapeutic agent for colon cancer and phosphorylation represents an effective method of enhancing the antitumor activity of CP.

关键词： Cyclocarya paliurus polysaccharide Phosphorylated Colorectal cancer mitochondrial apoptosis pathway

Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways

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Journal of Oceanology and Limnology 2023年第6期41卷 2429-2443页

作者： Shengyu LUO Cheng LIU Xinming GAO Jingqian WANG Yibo ZHANG Jie DING Congcong HOU Junquan ZHU Bao LOU Weiliang SHEN Xiongfei WU Chundan ZHANG Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Zhejiang Academy of Agricultural SciencesHangzhou 310021China State Key Laboratory of Large Yellow Croaker Breeding Ningbo Academy of Oceanology and FisheryNingbo 315012China

Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea,an economically important mariculture fish in *** is a consequence of hypoxia on ***,the effects of hypoxia stress on apoptosis in *** remain largely *** investigated the effect of environmental hypoxia on apoptosis in *** show that hypoxia induced apoptosis in *** both in vivo and in *** mitochondrial membrane potential was significantly reduced in large yellow croaker fry(LYCF)*** expression levels of Bcell lymphoma/leukemia-2(Bcl-2)m RNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress,respectively,whereas the expression level of Bcl-2 associated X(Bax)mRNA,Casp3 mRNA,and activity of caspase-3/7/9 were significantly increased,indicating that hypoxia induced caspase-dependent intrinsic apoptosis in *** expression level of the apoptosis-inducing factor(AIF)protein was significantly increased in the liver and LYCF *** level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of ***,demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic *** addition,the activity of caspase-8 was significantly increased,indicating that hypoxia stress induced extrinsic apoptosis in ***,hypoxia induced apoptosis in *** through both the intrinsic and extrinsic *** present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish.

关键词： hypoxia large yellow croaker mitochondrial apoptosis pathway death receptor apoptosis pathway

HSP70 inhibitor combined with cisplatin suppresses the cervical cancer proliferation in vitro and transplanted tumor growth:An experimental study

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Asian Pacific Journal of Tropical Medicine 2017年第2期10卷 177-181页

作者： Jian Liu Jing Liu Su-Yang Guo Hong-Li Liu Sheng-Ze Li Gynecological Oncology the First Affiliated Hospital of Bengbu Medical CollegeBengbu City 233004Anhui ProvinceChina

Objective:To study the regulating effect of HSP70 inhibitor(PES) combined with cisplatin on cervical cancer proliferation in vitro and transplanted tumor ***:Cervical cancer Hela cell lines were cultured and divided into control group,cisplatin group,PES group and cisplatin+PES group that were treated with serum-free DMEM,cisplatin with final concentration of 10 μmol/L,PES 20 μmol/L and cisplatin 10 μmol/L combined with PES with 20 μmol/L,respectively;animal models with cervical cancer xenografts were established and divided into control group,cisplatin group,PES group and cisplatin+PES group who received intra-tumor injection of normal saline,10 μmol/L cisplatin,20 μmol/L PES as well as 10 μmol/L cisplatin+20 μmol/L PES,*** proliferation activity,transplanted tumor volume and mitochondria apoptosis molecule expression were ***:Cell viability value and Bcl-2 mRNA expression in cells of cisplatin group,PES group and cisplatin+PES group were significantly lower than those of control group while Bax,Caspase-3 and Caspase-9 mRNA expression in cells were significantly higher than those of control group;transplanted tumor volume and the Bcl-2 mRNA expression in transplanted tumor tissue of cisplatin group,PES group and cisplatin+PES group were significantly lower than those of control group while Bax,Caspase-3 and Caspase-9 m RNA expression in transplanted tumor tissue were significantly higher than those of control ***:HSP70 inhibitor combined with cislatin can inhibit cervical cancer cell proliferation in vitro and transplanted tumor growth through mitochondrial apoptosis pathway.

关键词： Cervical cancer Heat shock protein 70 Transplanted tumor mitochondrial apoptosis pathway

Combination of Total Astragalus Extract and Total Panax Notoginseng Saponins Strengthened the Protective Effects on Brain Damage through Improving Energy Metabolism and Inhibiting apoptosis after Cerebral Ischemia-Reperfusion in Mice

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Chinese Journal of Integrative Medicine 2017年第6期23卷 445-452页

作者： HUANG Xiao-ping TAN Hua CHEN Bei-yang DENG Chang-qing Key Laboratory of Hunan Universities for Cell Biology and Molecular Techniques Key Laboratory of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardio-Cerebral DiseasesHunan University of Chinese MedicineChangsha 410007China Department of Histology and Embryology Hunan University of Chinese MedicineChangsha 410208China

Objective： To explore the effects and molecular mechanisms of the combination between total Astragalus extract （TAE） and total Panax notoginseng saponins （TPNS） against cerebral ischemia- reperfusion injury. Methods： C57BL/6 mice were randomly divided into sham-operated group, model group, TAE （110 mg/kg） group, TPNS （115 mg/kg） group, TAE-TPNS combination group and Edaravone （4 mg/kg） group, treated for 4 days, then, cerebral ischemia-repeffusion injury was established by bilateral common carotid artery （CCA） ligation for 20 min followed by reperfusion for 1 and 24 h. Results： TPNS could increase adenosine triphosphate （ATP） level, TAE and TAE-TPNS combination increased ATP, adenosine diphosphate （ADP） contents and Na＋-K＋-ATPase activity, and the effects of TAE-TPNS combination were stronger than those of TAE or TPNS alone after reperfusion for 1 h. After reperfusion for 24 h, TAE, TPNS and TAE-TPNS combination significantly increased neurocyte survival rate and decreased the apoptosis rate as well as down-regulated the expression of phosphorylated c-June N-terminal kinasel/2 （p-JNK1/2）, cytochrome C （Cyt C）, cysteine aspartic acid-specific protease （Caspase）-9 and Caspase-3. Furthermore, the effects in TAE-TPNS combination were better than those in TAE or TPNS alone. Conclusion： The combination of TAE 110 mg/kg and TPNS 115 mg/kg could strengthen protective effects on cerebral ischemia injury, the mechanism underlying might be related to improving jointly the early energy metabolism, and relieving the delayed apoptosis via inhibiting the mitochondrial apoptosis pathway of JNK signal transduction.

关键词： total Astragalus extract total Panax notoginseng saponins combination cerebral ischemia- reperfusion energy metabolism C-Jun N-terminal kinase signal transduction mitochondrial apoptosis pathway Chinese medicine

Comparative studies on energy metabolism and neurocyte apoptosis among Astragalosides,Panax notoginseng saponins and their combinations after cerebral ischemia-reperfusion in mice

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Comparative studies on energy metabolism and neurocyte apopt...

apoptosis mechanism of gastric cancer cell SGC-7901 induced by Omphalia lapidescens protein pPeOp

中南地区第八届生理学学术大会

作者： Xiao-Ping Huang a , Hua Tan a , Bei-Yang Chen b , Chang-Qing Deng a. a. Pathophysiology Laboratory, Hunan University of Chinese Medicine, Changsha, Hunan, P.R. China b. Department of Histology and Embryology, Hunan University of Chinese Medicine, Changsha, Hunan Province, PR. China

Astragalus and Panax notoginseng are often combined to treat cerebral infarction. Astragalosides （AST） and Panax notoginseng saponins （PNS） are the effective components with cardio-cerebrovascular pharmacological effects in Astragalus and Panax notoginseng, respectively, they both may antagonize ischemic brain injury. The purpose of this work was to compare the protective effects of AST, PNS and their combinations against cerebral ischemia injury through energy metabolism and neurocyte apoptosis. C56BL/6 mice were randomly divided into sham group, model group, AST （55 mg/kg） group, PNS （57.5 mg/kg） group, small-dose combination group （AST 27.5 mg/kg + PNS 28.75 mg/kg）, moderate-dose combination group （AST 55mg/kg + PNS 57.5mg/kg）, large-dose combination group （AST 110 mg/kg + PNS 115 mg/kg） and edaravone （4 mg/kg） group, treated for 4 days, then, cerebral ischemia-reperfusion injury was established by bilateral common carotid artery （CCA） ligation for 20 min followed by reperfusion. Our results showed that AST, PNS and their combinations increased the levels of ATP, ADP and Na + -K + -ATPase activity after reperfusion for 1 h. Twenty-four and 48 h after reperfusion, AST, PNS and the combinations significantly increased neurocyte survival and decreased the apoptosis rate as well as down-regulated the expression of phosphorylated c-June N-terminal kinase1/2 （p-JNK1/2）, cytochrome C （Cyt C）, cysteine aspartic acid-specific protease （caspase）-9 and caspase-3. Furthermore, the effects on energy metabolism and apoptosis were dose dependent among smalllow-dose combination group, moderate-dose combination group and large-dose combination group, and being better in moderate-dose combination group than those in AST or PNS group alone. It suggested that AST, PNS and their combinations had protective effects against cerebral ischemia injury, and the combination between AST and PNS could intensify the effect, the mechanism underlying might be associated with improving energy met

关键词： Astragalosides Panax notoginseng saponins combination cerebral ischemia-reperfusion energy metabolism c-Jun N-terminal kinase1/2 signaling pathway mitochondrial apoptosis pathway

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Apoptosis mechanism of gastric cancer cell SGC-7901 induced ...