Yi Qi Jie Du Decoction Inhibits Proliferation and Induces Apoptosis of Nasopharyngeal Carcinoma Stem Cells Through Mitochondrial Apoptosis Pathway

作     者：ZHOU Fang-Liang HE Lan HE Dan WANG Xian-Wen SHI Hong-Jian HE Ying-Chun CAO De-Liang 周芳亮;何兰;何丹;王贤文;史红健;何迎春;CAO De-Liang

作者机构：Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese MedicineHunan University of Chinese MedicineChangshaHunan 410208China School of MedicineHunan University of Chinese MedicineChangshaHunan 410208China Department of Medical MicrobiologyImmunology&Cell BiologySimmons Cancer InstituteSouthern Illinois University School of MedicineSpringfield 62702USA 

出 版 物：《Digital Chinese Medicine》 (数字中医药（英文）)

年 卷 期：2019年第2卷第4期

页      面：219-226页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：funding support from the National Natural Science Foundation of China (No. 81573721, No. 81874408 and No. 81973914) the Natural Science Foundation of Hunan (No. 2017JJ3246 and No. 2019JJ40216) Excellent Youth Fund of Hunan Provincial Education Department (No. 19B439) 

主　　题：Nasopharyngeal carcinoma(NPC) Yi Qi Jie Du Decoction(YQJDD) Apoptosis Cancer stem cells Mitochondrial apoptosis pathway 

摘      要：Objective To assess the effects of Yi Qi Jie Du Decoction(YQJDD)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying *** NPC-SCs were collected in serum-free culture system and identified by the sphere formation *** effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 *** change of mitochondrial membrane potential(ΔΨm)in NPC-SCs following treatment with YQJDD was investigated by JC-1 *** levels of apoptosisassociated proteins were examined using western blot *** YQJDD significantly inhibited the proliferation of NPCSCs in dose-and time-dependent ***-1 staining revealed that YQJDD lowered theΔΨm of NPC-SCs in a timedependent *** treatment with YQJDD,the expression of cleaved caspase-3,-7 and-9,cleaved poly-ADP ribose polymerase,P21 and P53 were increased,while the expression of Survivin in NPC-SCs was ***,the expression of cleaved caspase-8 remained nearly *** YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.