Fine particulate matters inducing reproductive impairment by causing cell cycle arrest and activating mitochondrial apoptosis pathway in male rats and spermatocyte cells
作者单位：Department of Toxicology and Hygienic ChemistrySchool of Public HealthCapital Medical University Beijing Key Laboratory of Environmental ToxicologyCapital Medical University
会议名称：《中国生理学会内分泌代谢、比较生理与应激生理学术会议》
会议日期：2017年
基 金：supported by Grants from Beijing Natural Science Foundation Program and(KZ201510025028) National Natural Science Foundation of China (81571130090)
关 键 词：Fine particulate matters oxidative stress cell cycle arrest mitochondrial apoptosis pathway male reproduction
摘 要：Fine particulate matters(PM2.5) have been shown to induce male reproductive toxicity,but the mechanism is *** explore the toxic mechanism of PM2.5,32 male Sprague-Dawley(SD)rats were treated to normal saline(control group) or PM2.5 with the doses of 1.8,5.4 and 16.2 mg/*** intratracheal instillation every 3 days for 30 *** 30 days,the rats were sacrificed for tissue *** vitro,GC-2 spd cells were treated to various concentrations(12.5,25,50 μg/mL) of PM2.5 for 24 *** results showed that PM2.5 obviously decreased sperm quality,increased sperm abnormality rate and damaged testicular tissue in male ***2.5 induced oxidative stress and DNA damage in ***,PM2.5 significantly increased the expression of BAX,cytochrome C,Caspase-9,Caspase-3 on the 30 th *** vitro,GC-2 cells treated with PM2.5 displayed mitochondria swelling and cristae ***2.5 induced S-phase cell cycle arrest and apoptosis in GC-2 spd *** results suggest that PM2.5 might induce apoptosis by up-regulating the expressions of mitochondrial apoptosis pathway factors through causing DNA damage and cell cycle arrest resulting from oxidative stress,and cause a decrease in the quantity and quality of sperms.