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Magnetic covalent organic framework nanospheres-based mirna biosensor for sensitive glioma detection

Bioactive Materials 2022年第8期7卷 145-151页

作者： Dong Liang Xiaoyi Zhang Yi Wang Taotao Huo Min Qian Yibo Xie Wenshuai Li Yunqiu Yu Wei Shi Qianqian Liu Junle Zhu Chun Luo Zhijuan Cao Rongqin Huang School of Pharmacy Key Laboratory of Smart Drug DeliveryMinistry of EducationFudan UniversityShanghai201203China Center for Advanced Low-dimension Materials State Key Laboratory for Modification of Chemical Fibers and Polymer MaterialsCollege of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai201600China Department of Neurosurgery Affiliated Hospital of Nantong UniversityNantong226001China Department of Neurosurgery Tongji HospitalTongji University School of MedicineShanghai200065China

Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent ***,dispersed magnetic covalent organic framework nanospheres(MCOF)with uniformed Fe3O4 nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth *** on the unique interaction between MCOF and hairpin DNA,a fluorescent signal amplified mirna biosensor was *** could realize the sensitive detection of mirna-182 in different matrixes,where the detection limit,linearity range and determination coefficient(R^(2))in real blood samples reached 20 fM,0.1 pM-10 pM and 0.991,***,it possessed good stability and precision as observed from the low intra-day/inter-day RSD and high extraction *** a result,it could quantify mirna-182 in serum of glioma patients,the concentration of which was significantly higher than that of healthy people and obviously decreased after ***,a proof-of-concept capillary chip system using this biosensor was proposed to realize the visualized detection of mirna-182 in *** findings suggest a robust way for sensitive detection and accurate diagnosis/prognosis of glioma.

关键词： Blood samples Fluorescence signal amplification biosensor Glioma diagnosis Magnetic COF nanospheres mirna detection

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Zeptomole Imaging of Cytosolic MicroRNA Cancer Biomarkers with A Light-Controlled Nanoantenna

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Nano-Micro Letters 2021年第12期13卷 468-482页

作者： Yang Song Xiaoli Cai Grayson Ostermeyer Shichao Ding Dan Du Yuehe Lin School of Mechanical and Materials Engineering Washington State UniversityPullmanWA 99164USA Nanosong Systems LLC RedmondWA 98052USA School of Biological Sciences Washington State UniversityPullmanWA 99164USA

Detecting and quantifying intracellular microRNAs(mirnas)are a critical step in resolving a cancer diagnostic and resolving the ensemble of gene products that orchestrate the living state of ***,the nanoprobe for detecting low abundance mirnas in cell cytosol is restricted by either the"one-to-one"signaltrigger model or di culty for cytosol *** address these challenges,we designed a lightharvesting nanoantenna-based nanoprobe,which directs excitation energy to a single molecule to sensitively detect cytosolic *** light irradiation,the light-harvesting nanoantenna e ectively disrupted lysosomal structures by generationof reactive oxygen species,substantially achieved cytosol *** nanoantenna containing>4000 donor dyes can e ciently transfer excitation energy to one or two acceptors with 99%e ciency,leading to unprecedented signal amplification and biosensing *** designed nanoantenna can quantify cytosolic miR-210 at zeptomolar *** fluorescence lifetime of the donor exhibited good relationship with miR-210 concentration in the range of 0.032 to 2.97 amol/*** zeptomole sensitivity of nanoantenna provides accurate bioimaging of miR-210 both in multiple cell lines and in vivo assay,which creates a pathway for the creation of mirna toolbox for quantitative epigenetics and personalized medicine.

关键词： Light-harvest Nanoantenna mirna detection Zeptomole Multiple cell lines

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3D SERS substrate based on nanocone forests for mirna detections

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Journal of Measurement Science and Instrumentation 2021年第2期12卷 226-231页

作者： LI Ruirui LI Yongwei XIONG Jijun Key Laboratory of Instrumentation Science and Dynamic Measuremen(North University of China) Ministry of Education Taiyuan 030051 China

Surface-enhanced Raman scattering(SERS)is a powerful technology for obtaining vibrational information from molecules that present in different chemical or biological *** paper presents a 3D SERS substrate based on nanocone *** substrates are prepared by using plasma treatment technique,which is a simple,fast and high-throughput *** SERS substrate based on nanocone forests exhibits high *** the experiment,mirna with a concentration as low as 10-10 M can be ***,the proposed SERS substrate shows a high uniformity over a large *** experimental results demonstrate great potential of the 3D SERS substrate in wide applications.

关键词： surface-enhanced Raman scattering(SERS)substrates nanocone forests three-dimensional hot spots mirna detection

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