Unraveling the interplay of DNAzyme and interfacial factors for enhanced biosensing

作     者：Yiyang Shen Zhen Zhang Ruyi Liang Tongbo Wu 

作者机构：The First Affiliated Hospitaland College of Clinical Medicine of Henan University of Science and TechnologyLuoyang 471003China School of PharmacyTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第12期

页      面：249-253页

核心收录：

学科分类：08[工学] 081101[工学-控制理论与控制工程] 0811[工学-控制科学与工程] 081102[工学-检测技术与自动化装置] 

基　　金：financially supported by the National Natural Science Foundation of China(Nos.82172372 and 82302651) the Training Program of Innovation and Entrepreneurship for Undergraduates of Hubei Province(No.S202210487267) 

主　　题：DNAzyme Gold nanoparticles Interfacial factor Multiple modulation functions miRNA detection 

摘      要：Deoxyribozyme(DNAzyme)and its substrate hybridization are crucial for achieving desirable detection performance in the DNAzyme coupling nanomaterial biosensor ***,interfacial factors such as electrostatic repulsion,steric hindrance,and nonspecific adsorption from gold nanoparticles make this hybridization process complicated and ***,the DNAzyme structure changes with different application purposes,which might affect the DNAzyme and substrate s *** studies have focused on the interplay of DNAzyme and interfacial factors in the biosensor *** this work,three types of DNAzyme variants were designed,and their biosensor performance rules were studied and summarized with the synergistic effect of interfacial ***,corresponding biosensor applications,such as multiple modulation functions and mi RNA detections,were constructed based on the distinct principles of DNAzyme variants.