Zeptomole Imaging of Cytosolic MicroRNA Cancer Biomarkers with A Light-Controlled Nanoantenna

作     者：Yang Song Xiaoli Cai Grayson Ostermeyer Shichao Ding Dan Du Yuehe Lin Yang Song;Xiaoli Cai;Grayson Ostermeyer;Shichao Ding;Dan Du;Yuehe Lin

作者机构：School of Mechanical and Materials EngineeringWashington State UniversityPullmanWA 99164USA Nanosong Systems LLCRedmondWA 98052USA School of Biological SciencesWashington State UniversityPullmanWA 99164USA 

出 版 物：《Nano-Micro Letters》 (纳微快报（英文版）)

年 卷 期：2021年第13卷第12期

页      面：468-482页

核心收录：

学科分类：1002[医学-临床医学] 08[工学] 080203[工学-机械设计及理论] 100214[医学-肿瘤学] 0802[工学-机械工程] 10[医学] 

基　　金：supported by start-up fund of Washington State University 

主　　题：Light-harvest Nanoantenna miRNA detection Zeptomole Multiple cell lines 

摘      要：Detecting and quantifying intracellular microRNAs(miRNAs)are a critical step in resolving a cancer diagnostic and resolving the ensemble of gene products that orchestrate the living state of ***,the nanoprobe for detecting low abundance miRNAs in cell cytosol is restricted by either theone-to-onesignaltrigger model or di culty for cytosol *** address these challenges,we designed a lightharvesting nanoantenna-based nanoprobe,which directs excitation energy to a single molecule to sensitively detect cytosolic *** light irradiation,the light-harvesting nanoantenna e ectively disrupted lysosomal structures by generationof reactive oxygen species,substantially achieved cytosol *** nanoantenna containing4000 donor dyes can e ciently transfer excitation energy to one or two acceptors with 99%e ciency,leading to unprecedented signal amplification and biosensing *** designed nanoantenna can quantify cytosolic miR-210 at zeptomolar *** fluorescence lifetime of the donor exhibited good relationship with miR-210 concentration in the range of 0.032 to 2.97 amol/*** zeptomole sensitivity of nanoantenna provides accurate bioimaging of miR-210 both in multiple cell lines and in vivo assay,which creates a pathway for the creation of miRNA toolbox for quantitative epigenetics and personalized medicine.