检索结果-南通市图书馆

medaka factor in the germline alpha(figα)is indispensable for oogenesis but not for gonadal sex differentiation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2024年第3期9卷 347-354页

作者： Katsuyasu Kamimura Masato Kinoshita Akira Kanamori Division of Biological Science Graduate School of ScienceNagoya UniversityNagoyaAichi464-8602Japan Division of Applied Biosciences Graduate School of AgricultureKyoto UniversityKyoto606-8502Japan

A basic helix-loop-helix transcription factor,figα,is one of the earliest marker genes of oocyte differentiation in *** the present study,we made figαknockout medaka by CRISPR/Cas9,expecting aborted progress of oogenesis,to see if differentiation of somatic ovarian tissues is ***αknockout male gonads differentiated normally into testes with functional *** females,on the other hand,were sterile;there are oocytes only up to *** growing oocytes in diplotene were *** phenotype was already apparent at 10 days after hatching,when diplotene oocytes start to develop in the ***,several putative target genes of figαwere not expressed in the mutant female *** studies showed that medaka lacking germ cells have morphologically abnormal gonads and female to male sex reversal ***αknockout female gonads differentiated morphologically into ovaries,suggesting that in medaka,figαknockout prevents oogenesis progress into diplotene stage,and ovarian differentiation do not need developed oocytes beyond pachytene *** ovarian phenotype reminded us of"pachytene checkpoint",which blocks meiotic progress into diplotene when chromosomal abnormalities are ***,we further analyzed phenotype of oocytes in the mutant *** number of oocytes at each meiotic stage suggested that oogenesis normally proceeded up to pachytene *** number of apoptotic oocytes in the knockout ovaries was ***,we did not detect any abnormal pairings or unrepaired double-strand breaks by *** together,figαknockout likely stop oocyte growth at or around pachytene via unknown mechanisms other than"pachytene checkpoint"and arrested oocytes remained in the *** is in contrast to figαknockout mice where all oocytes underwent apoptosis quickly after birth.

关键词： bHLH transcription factor CRISPR/Cas9 Pachytene checkpoint Oocyte growth medaka

medaka gcnf is a component of chromatoid body during spermiogenesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2021年第6期6卷 574-582页

作者： Zinan Xie Peng Song Ying Zhong Jing Guo Lang Gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

Germ cells of many organisms exhibit a unique perinuclear cytoplasmic cloud-like structure called the chromatoid body(CB).Specific components of the CB,such as vasa,are crucial for germline *** we identify another component of the CB,the Oryzias latipes gcnf homolog(Olgcnf).By reverse transcription polymerase chain reaction(RT-PCR),Olgcnf transcript was restricted to gonads in the adult tissues and was maternally provided and persisted throughout *** in situ hybridization on gonadal sections,Olgcnf transcript was restricted to germ *** the ovary,Olgcnf was predominantly expressed in the early stages of *** the testis,Olgcnf showed dynamic expression in round spermatids and sperm,accumulated in the CB during spermatid morphogenesis and concentrated in elongated spermatids of acrosome cap region and flagellar ***,Olgcnf was colocalized with vasa,which is the best-characterized component of the ***,Olgcnf is specific to germ cells of both sexes and is a component of the CB during spermiogenesis.

关键词： Gcnf medaka Ovary Testis

Genetic Diversity of Chinese medaka(Oryzias sinensis)in Dian Lake Drainage Area by ISSR Analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

湿地科学 2015年第5期13卷 543-550页

作者： QIU Yuping LIU Yong SUN Dacheng CHEN Guozhu National Plateau Wetland Research Center Southwest Forestry University College of Resources and Environmental Science Yunnan Agricultural University

Chinese medaka(Oryzias sinensis),widely distributed in the plateau lakes,Yunnan Province,which was the indicative specie for the habitat quality of riparian zone in the wetland ecosystems and also was the key specie in the lake ecosystems,and also was one of the fishes who suffered the severe invasion of alien ***,we investigated that the genetic diversity and population structure of the wild Chinese medaka by using inter-simple sequence repeat(ISSR)*** samples(20 individuals in total)of Chinese medaka in three populations in the drainage area of Dian Lake were *** 7 ISSR primers were picked out to assess the genetic *** ISSR primers combinations revealed 82.69%polymorphism,among 52 genetic loci *** sum of effective number of alleles and observed number of alleles were 1.492 4 and 1.826 9,*** Shannon genetic diversity and Nei genetic diversity indexes of all the populations were 0.434 5 and 0.289 7,*** gene differentiation(GST)was 0.198 9,and the gene flow(NM)was 2.013 *** AMOVA demonstrated that only 6.65%of genetic variance came from inter-population,and 93.35%of genetic variance came from *** tree showed that 20 individuals from 3 populations were clustering mixed each ***,the principal coordinate analysis(PCA)supported the cluster analysis *** results indicated that the genetic diversity of Oryzias sinensis was at the middle level,and there was no obvious genetic differentiation among the three populations of Oryzias sinensis.

关键词： Oryzias sinensis Chinese medaka genetic diversity

Characterization and expression analysis of sox3 in medaka gonads

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2022年第1期7卷 23-30页

作者： Quan Pu Yuan Ma Ying Zhong Jing Guo Lang Gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

Numerous studies have showed that sox3 is involved in neurogenesis and sex differentiation in ***,the accurate expression pattern is still unclear in *** this study,medaka sox3 was isolated and its expression patterns were compared with germ cell gene vasa in adult *** reverse transcription-polymerase chain reaction(RT-PCR)analysis,sox3 RNA expression was detected in the brain,eyes and *** chromogenic and fluorescent in situ hybridization,sox3 was present in the oogonia and early stages of oocytes as well as granulosa cells and theca cells in the later stages;in the testis,sox3 was occurred in the Sertoli cells and sperm with a specific signal in the location of chromatid body of sperm;in the embryos,sox3 was expressed in the central *** results suggest that medaka sox3 gene was occurred in somatic and germ cells in adult gonads of both sexes,involved in the process of spermatogenesis,as well as the development of the nervous *** study provides a precise expression pattern of sox3 and suggests that sox3 may be involved in gonadal and nervous system development of fish.

关键词： medaka sox3 Gonad development Nervous system development

Characterization and expression analysis of gonad specific igf3 in the medaka ovary

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2022年第3期7卷 259-268页

作者： Jiale Xie Ying Zhong Yuli Zhao Wenjie Xie Jing Guo Lang Gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityChina Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

The gonad specific expression of insulin-like growth factor 3(igf3)has suggested an important role of igf3 in fish *** this study,medaka igf3 was isolated and its expression patterns were compared with igf1,and germ cell gene vasa in adult medaka *** cloning and sequencing showed that the open reading frame(ORF)of medaka igf3 was comprised of 501 nucleotides and encoded 166 amino acid *** transcription polymerase chain reaction(RT-PCR)analysis showed that igf3 RNA was specifically expressed in developing embryos and adult gonads of both *** time quantitative PCR(q-PCR)indicated that igf3 expression was gradually increased during oogenesis,and reached its highest level at stage *** Chromogenic and fluorescent in situ hybridization,igf3 was shown to be present in the germ cells of oogonia and in oocytes at stages I-III,as well as abundant in granulosa cells and theca cells of oocytes at stages *** addition,igf3 and igf1 were expressed mutually in the outer theca cells of stage V oocytes in ***,we demonstrated that gonad specific igf3 could mark medaka ovarian somatic cells and germ *** findings highlight the importance of igf3 during ovarian development.

关键词： igf3 igf1 medaka Ovary

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

双酚-A对medaka的类雌激素效应研究

环境科学学报 2005年第11期25卷 1550-1554页

作者：周群芳江桂斌中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室北京100085

利用medaka进行了双酚-A与17β-雌二醇的短期暴露研究.研究结果发现实验室建立的流水式暴露体系可为实验提供连续、恒定的污染物暴露浓度.利用肝肉指数可灵敏响应和指示污染物暴露.双酚-A可以剂量相关的方式诱导雄性medaka肝脏产生卵黄... 详细信息

利用medaka进行了双酚-A与17β-雌二醇的短期暴露研究.研究结果发现实验室建立的流水式暴露体系可为实验提供连续、恒定的污染物暴露浓度.利用肝肉指数可灵敏响应和指示污染物暴露.双酚-A可以剂量相关的方式诱导雄性medaka肝脏产生卵黄蛋白原,从而有效证实了其类雌激素效应.

关键词：双酚-A medaka ELISA 卵黄蛋白原

纳米硒在medaka（Oryzias latipes）体内的超富集及其氧化应激效应研究

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

纳米硒在Medaka（Oryzias latipes）体内的超富集及其氧化应激效...

纳米银与离子态银对medaka氧化应激损伤效应的比较研究

第五届全国环境化学大会

作者：李鸿程张劲松江桂斌中国科学院生态环境研究中心中国科学技术大学化学与材料科学学院

＜正＞硒（Se）是动物体内不可缺少的微量营养元素之一,对动物的生长发育和抗氧化机能发挥着重要的作用。动物体补充硒元素的主要途径是通过食物摄取方式,但是硒的营养剂量和其毒性剂量非常接近,稍有不慎易导致硒中毒。近年来,

关键词：纳米硒生物富集氧化应激 medaka

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

纳米银与离子态银对medaka氧化应激损伤效应的比较研究

三种纳米银对medaka胚胎与卵黄鱼发育的毒理学效应研究

中国毒理学会第五次全国学术大会

作者：吴源李鸿程刘伟骆文茹周群芳江桂斌中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室安徽医科大学公共卫生学院

目的纳米银已被广泛应用于电子、涂料、食品添加剂、化妆品、医疗器械和药物载体等,但是纳米银的生物安全性尚未得到足够的评价。通过比较研究纳米银和离子态银对胚胎期medaka氧化应激和氧化损伤效应,从而评价纳米银的毒性作用机理和毒... 详细信息

目的纳米银已被广泛应用于电子、涂料、食品添加剂、化妆品、医疗器械和药物载体等,但是纳米银的生物安全性尚未得到足够的评价。通过比较研究纳米银和离子态银对胚胎期medaka氧化应激和氧化损伤效应,从而评价纳米银的毒性作用机理和毒作用途径。方法水相氧化还原法制备纳米银颗粒,经多级洗涤、离心、纯化、干燥得纳米银粉末。以胚胎期medaka为动物模型,持续暴露9天,分别于暴露第1、3、5、7和第9天采集样本,分别测定卵内LDH、MDA、SOD、ROS、CAT、GPx和还原性GSH的含量变化,OriginPro 7.5拟合剂量-暴露曲线和时间-暴露曲线。结果紫外-可见分光光度计扫描、TEM及XRD数据表明自制的纳米银为球形颗粒,λmax=400 nm,单质银粉末纯度大于99%;粒径在30-35 nm,水溶液分散均一。纳米银暴露增加卵内LDH含量、SOD活性、MDA含量,而卵内还原性GSH和ROS呈下降趋势,并呈现剂量-效应关系,但是卵内CAT及GPx活性无明显变化。时间-效应拟合曲线则显示暴露第五天达到毒性效应最高峰,此后反应趋于稳定。离子态银暴露组则表明各时期均呈现明显的剂量-反应关系,而时间-效应关系则不明显,毒性作用剂量大约为纳米银毒性的100倍。结论无论是纳米银还是离子态银均能造成胚胎期medaka氧化应激状态,药物暴露造成的细胞缺氧引起LDH代偿性增高,并诱发SOD活性增加,并最终造成脂质过氧化。但是纳米银和离子态银表现出不同的剂量-反应关系和时间-效应反应关系,从而说明两种形态银的作用途径和模式并不完全一样,因而纳米银的毒性作用并不能简单归因于银离子的释放。

关键词：纳米银银离子 medaka 氧化损伤

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

三种纳米银对Medaka胚胎与卵黄鱼发育的毒理学效应研究