Characterization and expression analysis of sox3 in medaka gonads
作者机构：International Research Center for Marine BiosciencesMinistry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic ResourcesMinistry of EducationShanghai Ocean UniversityShanghai201306China
出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)
年 卷 期：2022年第7卷第1期
页 面：23-30页
基 金：the National Key R&D Program of China(2018YFD0901205) National Natural Science Foundation of China(31672700)
主 题：Medaka sox3 Gonad development Nervous system development
摘 要：Numerous studies have showed that sox3 is involved in neurogenesis and sex differentiation in ***,the accurate expression pattern is still unclear in *** this study,medaka sox3 was isolated and its expression patterns were compared with germ cell gene vasa in adult *** reverse transcription-polymerase chain reaction(RT-PCR)analysis,sox3 RNA expression was detected in the brain,eyes and *** chromogenic and fluorescent in situ hybridization,sox3 was present in the oogonia and early stages of oocytes as well as granulosa cells and theca cells in the later stages;in the testis,sox3 was occurred in the Sertoli cells and sperm with a specific signal in the location of chromatid body of sperm;in the embryos,sox3 was expressed in the central *** results suggest that medaka sox3 gene was occurred in somatic and germ cells in adult gonads of both sexes,involved in the process of spermatogenesis,as well as the development of the nervous *** study provides a precise expression pattern of sox3 and suggests that sox3 may be involved in gonadal and nervous system development of fish.
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