Medaka fish stem cells and their applications

作     者：YI MeiSheng HONG Ni LI ZhenDong YAN Yan WANG DanKe ZHAO HaoBin HONG YunHan 

作者机构：Department of Biological SciencesNational University of Singapore10 Kent Ridge CrescentSingapore 119260Singapore Division of BioengineeringSchool of Chemical and Biomedical EngineeringNanyang Technological UniversitySingapore 637457Singapore Hubei Key Laboratory of Genetic Regulation and Integrative BiologySchool of Life SciencesHuazhong Normal UniversityWuhan 430079China State Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of HydrobiologyChinese Academy of SciencesWuhan 430072China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2010年第53卷第4期

页      面：426-434页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：supported by the Biomedical Research Council of Singapore (Grant Nos. R-05-1-21-19-404, R-08-1-21-19-585 and SBIC-SSCC-002-2007) the Ministry of Education of Singapore (Grant No. R-154-000-285-112) the National University of Singapore (Grant No. R-154-000-153-720) 

主　　题：medaka stem cells pluripotency chimera nuclear transplant semi-cloning 

摘      要：Stem cells are present in developing embryos and adult tissues of multicellular organisms. Owing to their unique features, stem cells provide excellent opportunities for experimental analyses of basic developmental processes such as pluripotency control and cell fate decision and for regenerative medicine by stem cell-based therapy. Stem cell cultures have been best studied in 3 vertebrate organisms. These are the mouse, human and a small laboratory fish called medaka. Specifically, medaka has given rise to the first embryonic stem (ES) cells besides the mouse, the first adult testis-derived male stem cells spermatogonia capable of test-tube sperm production, and most recently, even haploid ES cells capable of producing Holly, a semi-cloned fertile female medaka from a mosaic oocyte created by microinjecting a haploid ES cell nucleus directly into a normal oocyte. These breakthroughs make medaka a favoring vertebrate model for stem cell research, the topic of this review.