检索结果-南通市图书馆

Effect of intrauterine hypoxia and Angelica sinensis injection on dentate gyrus neurons and learning and memory in juvenile rats

引用

Neural Regeneration Research 2009年第3期4卷 218-224页

作者： Feng Zhao Yi Han Jing Ma Hong YU Yan He Xiaoming Zhong Department of Histology and Embryology Luzhou Medical College Luzhou 646000 Sichuan Province China Department of Pediatrics the First-Affiliated Hospital of Gannan Medical College Ganzhou 341000 Jiangxi Province China

BACKGROUND： The present study analyzed the effect of 3 days （2 h/d） intrauterine hypoxia on learning and memory in juvenile rats, as well as the therapeutic effects of Angelica sinensis on dentate gyrus neurons, as well as learning and memory. OBJECTIVE： To explore the effects of intrauterine hypoxia on hippocampal dentate gyrus neurons, as well as learning and memory, in juvenile rats; to explore N-methyI-D-aspartate receptor-1 （NMDAR1） expression in the dentate gyrus of neonatal rats following intrauterine hypoxia, as well as prolonged hypoxia; to investigate the regulatory mechanisms of Angelica sinensis. DESIGN, TIME AND SETTING： A randomized and controlled experiment based on developmental neurobiology was performed at the Department of Histology and Embryology in Luzhou Medical College from October 2007 to October 2008. MATERIALS： Angelica sinensis solution （250 g/L） was obtained from Central South Hospital of Wuhan University, China. Neuron-specific enolase and NMDAR1 mRNA in situ hybridization reagents were provided by Wuhan Boster Biological Technology, China. Image-Pro Plus 6.0 analysis system was purchased from Media Cybernetics, USA. METHODS： Healthy pregnant Sprague Dawley rats （n = 30） were randomly divided into control （n = 10）, hypoxia （n = 10）, and Angelica （n = 10） groups. The Angelica and hypoxia pregnant rats were placed in a three-gas incubator （oxygen concentration： 13%） starting with day 14 of pregnancy for 2 hours/day for 5 consecutive days to establish a fetal rat intrauterine hypoxia model. One hour prior to modeling, the pregnant rats from the Angelica and hypoxia groups received Angelica sinensis and normal saline （8 mL/kg） injections, respectively, through the caudal vein. The control group procedures were identical to the hypoxia group, but lacked the hypoxic conditions. MAIN OUTCOME MEASURES： Brain tissues of neonatal rats were used to detect expression of NMDAR1 mRNA, and brain tissues of juvenile rats aged 30 days were used to determ

关键词： Angelica sinensis hypoxia hippocampus N-methyI-D-aspartate receptor-1 learning memory

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Distinct roles of A kinase anchoring protein in Drosophila olfactory learning and memory

Distinct roles of A kinase anchoring protein in Drosophila o...

引用

中国神经科学学会第六届学术会议暨学会成立十周年庆祝大会

作者： Tim Tully Cold Spring Harbor Laboratory P.O.Box 100 New York 111724

＜正＞One of the challenges in the study of signal transduction pathways in neurons is to understand the way in which signals are restricted to subcellular compartments and how different signaling pathways interact. To explore these issues, we are investigating the role of A kinase anchoring proteins （AKAPs） in Drosophila olfactory learning and memory. AKAPs localize PKA specific subcellular locations and assemble PKA into signaling modules that include phosphodiesterases, phosphatases, ion channels and receptors. Based on our large-scale memory mutant screening, we obtained about twelve candidate memory mutants. One of them is 911046, temporarily named joay. Joay is a novel olfactory memory

关键词： learning memory anchoring protein signal pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of mannotriose on learning and memory-related signal protein expression in the corticosterone injured rat hippocampus neurocyte model

Effects of mannotriose on learning and memory-related signal...

引用

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Lina Zhang Guoqin Jin Department of Biochemistry College of Basic Medicine of Shanghai University of TCM

Objective:To investigate the effects of the mannotriose on learning and memory -related signal protein expression of rat hippocampus neurocyte injured model induced by high dose corticosterone(CORT) in vitro. Methods:The primary cultured SD rat hippocampus neurocyte were divided into five groups:(1) control group, (2) 10-4M CORT model group,(3) 10-4M CORT +3nM RU38486 group,(4) 10-4M CORT +0.1g/L mannotriose group,(5) 10-4M CORT +40mM aricept *** cell morphology and survival rate were observed by doublestained with fluoresce Syto-13 and *** cell viability was measured by MTT *** expression of GCR,BDNF and SGK protein were measured by western *** regulating effects of mannotriose on above indices of CORT model cell were also studied. Results:Compared with the control group,the survival rate and viability were obviously decreased in CORT model cell. The expression of GCR,BDNF and SGK protein in CORT model cell were also lower than those in the control group *** mannotriose,RU38486,and aricept group, however,could improve the abnormal changes of above indices in CORT model cell to varying degrees. Conclusions:Ru38486 which is an antagonist of GCR could enhance the survival rate and viability of CORT model cell, it proved that 10-4M CORT has an injured effect on the hippocampus *** as a positive control aricept as could significantly protect the hippocampus neurocyte from injury of high concentrations CORT,retarding learning and memory functional deterioration induced by *** regulatory mechanism of the mannotriose might be related to raise the expression of GCR,BDNF and SGK protein associated signal transduction pathway on learning and memory.

关键词： learning memory CORT GCR BDNF SGK mannotnose

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

JZ-V improved learning and memory impairment and accelerated perforant path-dentate gyrus LTP in intact animals

JZ-V improved learning and memory impairment and accelerated...

引用

中国神经科学学会第十届全国学术会议

作者： Qichen XIE Xi CHEN Xinmin LIU Yong XIE Tianxiu QIAN Siwei CHEN Xiaoying WANG Institute of Medicinal Pplant Development Chinese Medical Sciences Life Science and Biopharmatics Shenyang Pharmaceutical University

Objective The purposes of this study were to investigate the effect of JZ-V,a novel peptide neurotoxin isolated from the venom of spider Chilobrachys jingzhao and composed of 29 residues including six cysteines and three exposed tryptophan residues on learning and memory and *** learning and memory impairment was induced by beta-amyloid 1-42 and Scopolamine,and the behavior was evaluated by Morris water maze and Step-through experiments to investigate the effect of JZ-V on spatial leaning and memory and passive avoidance learning and *** Morris water maze test,the latency,swimming distance,the searching strategy were recorded and the error numbers and the latency were recorded in Step through *** addition,extracellular technique was used to determine the effect of JZ-V on LTP induction in medial perforant path dentate granule cell synapses,single- pulse stimulation was delivered to perforant path fibers in *** slice recording system,the Schaffer-CAl pathway LTP was recorded in 400 microm-thick transverse hippocampal *** JZ-V significantly enhanced LTP induction,the population spike was increased from180 ± 11%to about 332 ± 28%of control in perforant path-dentate gyrus pathway 20 minutes after,high frequency ***,the results from shce recording was quite difference with that of anesthetized rats,the slope of EPSP was increased from about 141 ± 12%to 159 ± 14%of control 10minues after high frequency *** behavior results,JZ-V significantly reduced escape latency and swimming distance in place navigation test,and it significantly prolonged passive avoidance latency and decreased the error numbers during 5 minutes in step-through *** JZ-V significantly improved impaired learning and memory induced by beta-amyloid and Scopolamine,which showed some potential to be a promising new trial drug for Alzheimer’s *** present study gave the strong evidence that learning and memory behavior is associat

关键词： LTP learning memory synaptic plasticity Spider Chilobrachys jingzhao

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of kidney-tonifying recipe on serum corticosterone and learning & memory-related NR1/NR2B mRNA expressions of aging rats

Effects of kidney-tonifying recipe on serum corticosterone a...

引用

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Xiangping Kang Guoqin Jin Shanghai University of TCM

Objective:To observe the effects of Kidney-tonifying recipe—Zuogui Wan and Yougui Wan on serum corticosterone(CORT) and learning & memory-related NR1/ NR2B mRNA expressions of aging rats. Methods:The nature old SD rats were used as senile animal *** rats were randomly divided into five groups: (l)young group(aged 5 month),(2)senile group(aged 24 month),(3) senile + antagonist RU38486(aged 24 month), (4)senile + Zuogui Wan group(aged 24 month),(5)senile + Yuogui Wan group(aged 24 month).The contents of

关键词： corticosterone learning memory LTP Kidney-tonifying recipe senile rat

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The human apoE7 and apoE4 transgenic mice models

引用

Science China(Life Sciences) 2001年第6期44卷 652-660页

作者： SUN Mingzeng ( 1. Department of Biochemistry Institute of Basic Medical Sciences CAMS & PUMC National Laboratory of Medical Molecular Biology 100005 Beijing China

To scrutinize the disorders caused by human mutant apoE7/apoE4, human apoE4 and E7 transgenic mice were established with microinjection technique to examine molecular genetic phenomena in vivo. The integration and expression of h-apoE mutant genes in transgenic mice were determined with Southern blot, Northern blot and ELISA. The current studies indicated that the transgenes and the phenotypes regarding expression of transgenes could be transmitted stably in transgenic lines. The levels of serum lipid in transgenic mice showed the characteristics of hyperlipidemia. Besides, behavior tests demonstrated the degeneration of learning and memory in transgenic mice. Short life span was observed in 2 transgenic lines. After fed with high lipid food high serum lipid was found both in normal and transgenic mice, but their mechanism regulating lipid metabolism was different. It was also verified that the human apoE mutants located at either N-terminal or C-terminal had the same pathogenesis regarding disorders of lipid metabolism in murine.

关键词： transgenic mice human apoE7/E4 hyperlipidemia learning memory life span.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anti-amnestic and anti-aging effects of ginsenoside Rg1 and Rb1 and its mechanism of action

引用

Acta Pharmacologica Sinica 2005年第2期26卷 143-149页

作者： Yong CHENG Li-hong SHEN Jun-tian ZHANG Institute of Materia Medica,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100050,China InstituteofMateriaMedica PekingUnionMedicalCollegeandChineseAcademyofMedicalSciencesBeijing100050China

In the present paper,we overview the discovery of new biological activitiesinduced by ginsenoside Rg1 and Rb1 and discuss possible mechanisms of *** compounds could increase neural plasticity in efficacy and structure;espe-cially Rg1,as one small molecular drug,can increase proliferation and differentia-tion of neural progenitor cells in dentate gyrus of hippocampus of normal adultmice and global ischemia model in *** finding has great value for treat-ment of Alzheimer’s disease and other neurodegenerative disorders which is char-acterized by neurons *** of expression of brain derived neurotrophicfactor,Bcl-2 and antioxidant enzyme,enhanced new synapse formation,inhibitionof apoptosis and calcium overload are also important neuron protective *** and Rbl have common effects,but there are some differences in pharmacol-ogy and *** differences may attribute to their different ***1 is panaxtriol with two sugars,while Rb1 is panaxtriol with foursugars.

关键词： ginseng ginsenoside-Rg1 ginsenoside-Rb1 learning memory neural progenitor cells apoptosis immunity synaptic plasticity muscarinic cholinergic receptor long-term potentiation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Ability of Tong Luo Jiu Nao Oral Solution to Improve Cognitive and Gait Deficits in a Rat Model of Focal Cerebral Ischemia

引用

Digital Chinese Medicine 2018年第1期1卷 67-83页

作者： Si-Di LI Yi-Hua SHENG Xin-Min LIU Zu-Guang YE Jishou University Xiangxi StateHunan 416000China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100007China The Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China

Objective To investigate the ability of Tong Luo Jiu Nao(TLJN)Oral Solution to improve cognitive and gait deficits in a rat model of focal cerebral *** An intraluminal suture method was used to create a rat model of focal cerebral *** animals were divided into4groups:sham surgery,cerebral ischemia model,cerebral ischemia model+low-dose TLJN Oral Solution(150mg/kg),cerebral ischemia model+high-dose TLJN Oral Solution(300mg/kg).After4weeks of drug intervention,water maze and gait analyses were *** tissue was stained with hematoxylin and eosin and used to monitor and calculate the volume ratio of cerebral infarction in each group of permanent middle cerebral artery occlusion(pMCAO)***(1)Gait analyses:TLJN Oral Solution significantly improved the overall gait of the focal cerebral ischemic rat *** improvement in walking speed might have been achieved by the shortening of the animals’stride *** shortening of stance time by TLJN in the cerebral ischemic animals might be due to the shortening of brake and propulsion times,while the shortening of stride time by TLJN in the cerebral ischemic animals might have been due to the shortening of stance time and swing time.(2)Water maze navigation test:high-dose TLJN Oral Solution significantly reduced the animals’total swimming distance and duration and increased the proportion of swimming distance and time spent in the target quadrant,suggesting that TLJN significantly improved the cognitive deficit in the animal model.(3)High-dose TLJN significantly reduced the cerebral infarction volume ratio of the pMCAO *** This intraluminal suture method can reliably replicate a rat model of focal cerebral *** Oral Solution improves the gait and cognitive function of the focal cerebral ischemia rat model to various extents.

关键词： Tong Luo Jiu Nao Focal cerebral ischemia Behavioristics Gait analyses learning memory

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

益智养神健脑丸对脑疲劳大鼠动物模型学习记忆能力及自由基的影响

益智养神健脑丸对脑疲劳大鼠动物模型学习记忆能力及自由基的影响

引用

第九次全国中西医结合诊断学术研讨会

作者：刘珊贾丹兵李乃民唐立明李春杰解放军第211医院

目的研究益智养神健脑丸对大鼠"脑疲劳"模型的记忆力及自由基的影响,为其进一步开发应用提供实验依据。方法利用声、光、电联合刺激法建立大鼠"脑疲劳"模型,采用造模同时给药和造模后给药两种方式,灌胃给予益智养神健脑丸原料药溶液,连... 详细信息

目的研究益智养神健脑丸对大鼠"脑疲劳"模型的记忆力及自由基的影响,为其进一步开发应用提供实验依据。方法利用声、光、电联合刺激法建立大鼠"脑疲劳"模型,采用造模同时给药和造模后给药两种方式,灌胃给予益智养神健脑丸原料药溶液,连续给药28天。观察各组大鼠给药前后一般状态和体重的变化,生化法检测血中一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果与模型组相比较,益智养神健脑丸能够明显改善模型大鼠学习记忆能力,升高血清中NO和SOD水平,降低MDA水平。结论益智养神健脑丸可能通过提高机体清除氧自由基的能力途径,从而改善"脑疲劳"模型大鼠学习记忆能力,达到明显的预防和治疗作用。

关键词： YIZHI YANGSHENG JIANNAOWAN Brain fatigue learning memory Free radicals

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

损毁伏核中央部阻断大鼠吗啡渴求,破坏关联性学习及冲动控制行为(英文)

引用

中国药物依赖性杂志 2010年第5期19卷 424-424页

作者： YU Peng,WANG Xinsheng,WANG Xuewei,CUI Cailian(Neuroscience Research Institute,Department of Neurobiology,School of Basic Medical Sciences,Key Lab for Neuroscience,the Ministry of Education and Ministry of Public Health,Peking University,38 Xueyuan Road,Beijing,100191,PR China)

Drug addiction has become a major public health problem worldwide. Conventional treatments can ameliorate the Abstinence symptoms effectively,but have little to do with relapsing. Recently,ablating the brain regions related to drug addiction permanently through stereotaxic surgery has been proposed and used as a new therapeutic strategy in China. This bold attempt is highly controversial,and major concerns are about the efficacy and side effects of the surgery. In the present study,morphine-induced conditioned place preference (CPP) was established in rats as an animal model to imitate the addicts’ psychological dependence or reflect the craving for the drug of abuse. Direct current (2 mA,7 s) was delivered to ablate sub-regions of bilateral nucleus accumbens (NAc) via a stainless microelectrode,which was similar to the stereotaxic surgery used clinically. To investigate the effect of bilateral NAc core lesions on morphine-induced CPP and the possible side effects on animals’ general physiological function,four behavioral models,Morris water maze,sucrose-induced CPP,open field test and delay discounting task,were applied to assess the rats’ spatial learning ability,associative learning ability,exploratory behavior and impulsive control ability,respectively after the surgical lesions. Results showed that lesions of the NAc core,but not the shell blocked the maintenance of morphine CPP in rats. Although the rats’ spatial learning ability was not significantly affected,their associative learning ability,exploratory behavior and impulsive control ability were obviously impaired by the operations. Our findings indicate that lesion of NAc core blocked the craving for morphine,while impairs associative learning and impulsive control abilities in rats,and that suggests lesion of the NAc core or shell should not be routinely used as a therapeutic strategy for the treatment of opiate addiction.

关键词： conditioned place preference electrolytic lesion impulsive control learning memory morphine nucleus accumbens

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：