检索结果-南通市图书馆

Ultrasensitive and point-of-care detection of plasma phosphorylated tau in Alzheimer’s disease using colorimetric and surface-enhanced Raman scattering dual-readout lateral flow assay

引用

Nano Research 2023年第5期16卷 7459-7469页

作者： Liding Zhang Ying Su Xiaohan Liang Kai Cao Qingming Luo Haiming Luo Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and Technology(HUST)Wuhan 430074China MoE Key Laboratory for Biomedical Photonics School of Engineering SciencesHuazhong University of Science and TechnologyWuhan 430074China Department of Neurology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430074China Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical EngineeringHainan UniversityHaikou 570228China Research Unit of Multimodal Cross Scale Neural Signal Detection and Imaging Chinese Academy of Medical SciencesHUST-Suzhou Institute for BrainsmaticsJiangsu Industrial Technology Research Institute(JITRI)Suzhou 215123China

Phosphorylation of tau at Ser(396,404)(p-tau^(396,404))is one of the earliest phosphorylation events,plasma p-tau^(396,404) level appears to be a potentially promising biomarker of Alzheimer’s disease(AD).The low abundance and easy degradation of p-tau in the plasma make the lateral flow assay(LFA)a suitable choice for point-of-care detection of plasma p-tau^(396,404) levels.Herein,based on our screening of a pair of p-tau^(396,404)-specific antibodies,we developed a colorimetric and surface-enhanced Raman scattering(SERS)dual-readout LFA for the rapid,highly sensitive,robust detection of plasma p-tau^(396,404) levels.This LFA realized a detection limit of 60 pg/mL by the naked eye or 3.8 pg/mL by SERS without cross-reacting with other tau species.More importantly,LFA rapidly and accurately differentiated AD patients from healthy controls,suggesting that it has the potential for clinical point-of-care application in AD diagnosis.This dual-readout LFA has the advantages of simple operation,rapid,ultra-sensitive detection,providing a new way for early AD diagnosis and intervention,especially in primary and community AD screening.

关键词： Alzheimer’s disease p-tau^(396,404) plasma detection surface-enhanced Raman scattering lateral flow assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

State of the art:lateral flow assay(LFA) biosensor for on-site rapid detection

引用

Chinese Chemical Letters 2018年第11期29卷 1567-1577页

作者： Shuai Zhao Sai Wang Shan Zhang Jiahui Liu Yiyang Dong College of Life Science and Technology Beijing University of Chemical Technology College of Food Science and Engineering Ocean University of China

Recently, lateral flow assay （LFA） has attracted researchers＇ attention because of its considerable advantages of superior portability, rapid detection, cost-effectiveness and ease of use. This review provides a brief overview of latest researches of LFA, including the practical use of LFA in both qualitative and quantitative analysis in different areas. Though bio-recognition molecules in the LFA used to be antibodies, a new kind of recognition element called aptamer, showing significant advantages, is developed rapidly in recent years. The highly specific recognition of aptamers/antibodies and targets are combined with the excellent properties of a dry-reagent strip biosensor that enables efficiently detection in point-of-care applications. Herein, we compared the aptamers with antibodies, summarized the principle of LFAs, and three main elements for the LFAs （recognition molecule, signal transduction element, the targets）. Additionally, we summarized different optimal experimental conditions in the recent LFA-related studies to give detailed overview of the LFA development. We hope the review can give a general guide for the development of LFAs.

关键词： lateral flow assay Aptamer Antibodies Recognition Signal transduction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A new quality control method for lateral flow assay

引用

Chinese Chemical Letters 2018年第12期29卷 1853-1856页

作者： Li Huang Di Zhang Lirong Jiao Enben Su Nongyue He State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University National Demonstration Center for Experimental Biomedical Engineering Education Southeast University Getein Biotechnology Co. Ltd.

Precision and repeatability are challenging issues in point of care testing(POCT) analysis. Herein, we proposed a lateral flow assay(LFA) based on internal quality control microspheres to realize the accurate diagnosis of HbAlc in human body. Fluorescein cy5 decorated microspheres are used as labels for HbAlc detection, and BSA-fluorescein isothiocyanate(FITC) decorated microspheres are used as internal quality control labels. One test line was employed in the strip for the detection of glycosylated hemoglobin(HbAlc). This method can eliminate the interference of environmental factors(temperature, humidity,etc.) to LFA in the process of chromatography, and improve the precision and accuracy of HbAlc detection.The CV for detection of low concentration HbAlc was 1.05%, and the CV for detection of high concentration HbAlc was 0.69%. We envision the method to have great prospect in in vitro diagnosis(IVD).

关键词： Point of care testing lateral flow assay HbA1c Fluorescein isothiocyanate Precision

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SERS lateral flow assay based on staining method for high-sensitive procalcitonin detection

SERS lateral flow assay based on staining method for high-se...

引用

第十一届全国化学生物学学术会议

作者： Haicong Shen Eunyeong Song Lingyan Meng Yang Wang Jing Dong Di Huang Zhi Zhu Chaoyong Yang State Key Laboratory of Physical Chemistry of Solid Surfaces the Key Laboratory for Chemical Biology of Fujian ProvinceThe MOE Key Laboratory of Spectrochemical Analysis & InstrumentationDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringXiamen University

Procalcitonin(PCT) works as a biomarker of sever bacterial infection for diagnosis,monitoring clinical effects and judging prognostic treatment .Therefore,it had a growing demand of development of low cost and portable methods for day to day clinical decision.As a rapid diagnostic testing performed at the sites near patients,point of care test(POCT) has recently aroused great interest for solving problems in global health.The research and innovation of microfluidic paper-based analytic device for POCT is particularly emphasized due to its advantages of rapidity,low-cost.Here we demonstrate SERS-based method on lateral flow test satisfying the need of PCT assay.

关键词： SERS PCT lateral flow assay

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Sensitively detecting antigen of SARS-CoV-2 by NIR-Ⅱ fluorescent nanoparticles

引用

Nano Research 2022年第8期15卷 7313-7319页

作者： Ruibin Hu Tao Liao Yan Ren Wenming Liu Rui Ma Xinyuan Wang Qihui Lin Guoxin Wang Yongye Liang Department of Materials Science and Engineering Southern University of Science and Technology of ChinaShenzhen 518055China WWHS Biotech.Inc. Shenzhen 518122China Joint Laboratory for Infectious Disease Prevention and Control Hygienic Section of Longhua Center for Disease Control and PreventionLonghua DistrictShenzhen 518109China

Early detection of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection is an efficient way to prevent the spread of coronavirus disease 2019(COVID-19).Detecting SARS-CoV-2 antigen can be rapid and convenient,but it is still challenging to develop highly sensitive methods for effective diagnosis.Herein,a lateral flow assay(LFA)based on fluorescent nanoparticles emitting in the second near-infrared(NIR-II)window is developed for sensitive detection of SARS-CoV-2 antigen.Benefiting from the NIR-II fluorescence with high penetration and low autofluorescence,such NIR-II based LFA allows enhanced signal-to-background ratio,and the limit of detection is down to 0.01 ng·mL^(−1)of SARS-CoV-2 antigen.In the clinical swab sample tests,the NIR-II LFA outperforms the colloidal gold LFA with higher overall percent agreement with the polymerase chain reaction test.The clinical samples with low antigen concentrations(~0.015–~0.068 ng·mL^(−1))can be successfully detected by the NIR-II LFA,but fail for the colloidal gold LFA.The NIR-II LFA can provide a promising platform for highly sensitive,rapid,and cost-effective method for early diagnosis and mass screening of SARS-CoV-2 infection.

关键词： coronavirus disease 2019(COVID-19) severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) antigen detection the second near-infrared(NIR-II)fluorophores lateral flow assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Novel Molecular Imprinted Polymers-Based lateral flow Strip for Sensitive Detection of Thiodiglycol

引用

Journal of Analysis and Testing 2023年第2期7卷 110-117页

作者： Yujie Luo Qichao Ye Tianjin Xie Jiali Xie Kai Mao Hongyan Zou Yuanfang Li Chengzhi Huang Shujun Zhen Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of Education College of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China Key Laboratory of Luminescent and Real-Time Analytical System(Southwest University) Chongqing Science and Technology BureauCollege of Pharmaceutical SciencesSouthwest UniversityChongqing 400715China

Traditional detection of thiodiglycol(TDG),a metabolic marker for sulfur mustard poisoning,requires not only professional operators,but also expensive reagents and large instruments.Herein,we developed a novel molecular imprinted polymers(MIPs)-based lateral flow assay(LFA)strategy for the quick,sensitive,and selective detection of TDG.Gold nanoparticles(Au NPs),MIPs,and metallothioneins(MTs)were respectively loaded on the conjugate pad,test line(T line)and control line(C line).After adding TDG,Au NPs on the conjugate pad reacted with TDG through the Au-S bond first.Then,under the action of capillary force,the conjugates of TDG and Au NPs were trapped by the MIPs as they traveled to the T line,and the residual Au NPs bound with the MTs on the C line,exhibiting two obvious red bands on T line and C line,respectively.In contrast,a single red band could be observed on C line without TDG.This method exhibited a wide linear range from 10.0 pg/m L to 10,000.0 ng/m L and its limit of detection(LOD)was as low as 0.41 pg/m L.This method was successfully utilized to detect TDG in human urine,presenting significant potential in the point-of-care testing of TDG in clinical samples of the sulfur mustard poisoning patients.

关键词： Molecular imprinted polymers lateral flow assay Thiodiglycol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A New Method for Blood NT-proBNP Determination Based on a Near-infrared Point of Care Testing Device with High Sensitivity and Wide Scope

引用

Biomedical and Environmental Sciences 2017年第6期30卷 426-431页

作者： ZHANG Xiao Guang SHU Yao Gen GAO Ju WANG Xuan LIU Li Peng WANG Meng CAO Yu Xi ZENG Yi National lnstitute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Beijing Runbio Biotechnology Development Co. Ltd Beijing 100106 China No.2 People's Hospital of Dali City Dali 671003 Yunnan China Department of Laboratory Medicine Beijing Hospital National Center of Gerontology Beijing 100730 China

Objective To develop a rapid, highly sensitive, and quantitative method for the detection of NT-proBNP levels based on a near-infrared point-of-care diagnostic （POCT） device with wide scope. Methods The lateral flow assay （LFA） strip of NT-proBNP was first prepared to achieve rapid detection. Then, the antibody pairs for NT-proBNP were screened and labeled with the near-infrared fluorescent dye Dylight-800. The capture antibody was fixed on a nitrocellulose membrane by a scribing device. Serial dilutions of serum samples were prepared using NT-proBNP-free serum series. The prepared test strips, combined with a near-infrared POCT device, were validated by known concentrations of clinical samples. The POCT device gave the output of the ratio of the intensity of the fluorescence signal of the detection line to that of the quality control line. The relationship between the ratio value and the concentration of the specimen was plotted as a work curve. The results of 62 clinical specimens obtained from our method were compared in parallel with those obtained from the Roche E411 kit. Results Based on the log-log plot, the new method demonstrated that there was a good linear relationship between the ratio value and NT-proBNP concentrations ranging from 20 pg/mL to 10 ng/mL. The results of the 62 clinical specimens measured by our method showed a good linear correlation with those measured by the Roche E411 kit. Conclusion The new LFA detection method of NT-proBNP levels based on the near-infrared POCT device was rapid and highly sensitive with wide scope and was thus suitable for rapid and early clinical diagnosis of cardiac impairment.

关键词： NT-proBNP Near-infrared POCT device lateral flow assay

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：