Sensitively detecting antigen of SARS-CoV-2 by NIR-Ⅱ fluorescent nanoparticles
作者机构：Department of Materials Science and EngineeringSouthern University of Science and Technology of ChinaShenzhen 518055China WWHS Biotech.Inc.Shenzhen 518122China Joint Laboratory for Infectious Disease Prevention and ControlHygienic Section of Longhua Center for Disease Control and PreventionLonghua DistrictShenzhen 518109China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2022年第15卷第8期
页 面：7313-7319页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 070205[理学-凝聚态物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 0702[理学-物理学]
基 金：Guangdong Provincial Department of Science and Technology-key research and development project(No.2020B1111160003) Shenzhen Science and Technology Innovation Commission technology breakthrough project(No.JSGG20191231141403880) Shenzhen San-Ming Project(No.SZSM201809085) Shenzhen Science and Technology Innovation Commission general project(No.JCYJ20180504165657443)。
主 题：coronavirus disease 2019(COVID-19) severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) antigen detection the second near-infrared(NIR-II)fluorophores lateral flow assay
摘 要：Early detection of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection is an efficient way to prevent the spread of coronavirus disease 2019(COVID-19).Detecting SARS-CoV-2 antigen can be rapid and convenient,but it is still challenging to develop highly sensitive methods for effective diagnosis.Herein,a lateral flow assay(LFA)based on fluorescent nanoparticles emitting in the second near-infrared(NIR-II)window is developed for sensitive detection of SARS-CoV-2 antigen.Benefiting from the NIR-II fluorescence with high penetration and low autofluorescence,such NIR-II based LFA allows enhanced signal-to-background ratio,and the limit of detection is down to 0.01 ng·mL^(−1)of SARS-CoV-2 antigen.In the clinical swab sample tests,the NIR-II LFA outperforms the colloidal gold LFA with higher overall percent agreement with the polymerase chain reaction test.The clinical samples with low antigen concentrations(~0.015–~0.068 ng·mL^(−1))can be successfully detected by the NIR-II LFA,but fail for the colloidal gold LFA.The NIR-II LFA can provide a promising platform for highly sensitive,rapid,and cost-effective method for early diagnosis and mass screening of SARS-CoV-2 infection.
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