A Novel Molecular Imprinted Polymers-Based Lateral Flow Strip for Sensitive Detection of Thiodiglycol

作     者：Yujie Luo Qichao Ye Tianjin Xie Jiali Xie Kai Mao Hongyan Zou Yuanfang Li Chengzhi Huang Shujun Zhen 

作者机构：Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University)Ministry of Education College of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China Key Laboratory of Luminescent and Real-Time Analytical System(Southwest University)Chongqing Science and Technology BureauCollege of Pharmaceutical SciencesSouthwest UniversityChongqing 400715China 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2023年第7卷第2期

页      面：110-117页

核心收录：

学科分类：07[理学] 070305[理学-高分子化学与物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：supported by the National Key Research and Development Program of China(2018YFC1602600) the National Natural Science Foundation of China(21974109) the Natural Science Foundation of Chongqing(CSTB2022NSCQ-MSX1662) 

主　　题：Molecular imprinted polymers Lateral flow assay Thiodiglycol 

摘      要：Traditional detection of thiodiglycol(TDG),a metabolic marker for sulfur mustard poisoning,requires not only professional operators,but also expensive reagents and large instruments.Herein,we developed a novel molecular imprinted polymers(MIPs)-based lateral flow assay(LFA)strategy for the quick,sensitive,and selective detection of TDG.Gold nanoparticles(Au NPs),MIPs,and metallothioneins(MTs)were respectively loaded on the conjugate pad,test line(T line)and control line(C line).After adding TDG,Au NPs on the conjugate pad reacted with TDG through the Au-S bond first.Then,under the action of capillary force,the conjugates of TDG and Au NPs were trapped by the MIPs as they traveled to the T line,and the residual Au NPs bound with the MTs on the C line,exhibiting two obvious red bands on T line and C line,respectively.In contrast,a single red band could be observed on C line without TDG.This method exhibited a wide linear range from 10.0 pg/m L to 10,000.0 ng/m L and its limit of detection(LOD)was as low as 0.41 pg/m L.This method was successfully utilized to detect TDG in human urine,presenting significant potential in the point-of-care testing of TDG in clinical samples of the sulfur mustard poisoning patients.