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intravenous injection of mesenchymal stem cells is effective in treating liver fibrosis

World Journal of Gastroenterology 2012年第10期18卷 1048-1058页

作者： Wei Zhao Jun-Jie Li Da-Yong Cao Xiao Li Lin-Ying Zhang Yong He Shu-Qiang Yue De-Sheng Wang Ke-Feng Dou Department of Hepatobiliary Surgery Xijing HospitalThe Fourth Military Medical UniversityXi'an 710032Shaanxi ProvinceChina

AIM: To compare the influence of different transplant sites in bone marrow mesenchymal stem cell (MSC)-based therapy for liver fibrosis. METHODS: MSCs isolated from Sprague Dawley (SD) rats were induced into hepatocyte-like cells. Liver fibrosis in SD rats was induced with carbon tetrachloride. Following hepatocyte induction in vitro, 4',6-diamidino- 2-phenylindole (DAPI)-labeled MSCs were transplanted by intravenous, intrahepatic, and intraperitoneal injection. Histopathological staining, immunohistochemistry, and biochemical analysis were used to compare the morphological and functional liver regeneration among different MSC injection modalities. The expression differences of interleukins, growth factor, extracellular matrix, matrix metalloproteinases, and tissue inhibitor of metalloproteinase were examined by real-time reverse transcription-polymerase chain reaction (RT-PCR) andenzyme linked immunosorbent assay (ELISA). RESULTS: Four days after exposure to hepatocyte differentiation medium, MSCs that did not express hepatocyte markers could express α-fetoprotein, albumin, and cytokeratin 18. The results of histopathological staining, immunohistochemistry, and biochemical analysis indicated that intravenous injection is more effective at rescuing liver failure than other injection modalities. DAPI-labeled cells were found around liver lobules in all three injection site groups, but the intravenous group had the highest number of cells. PCR and ELISA analysis indicated that interleukin-10 (IL-10) was highest in the intravenous group, whereas il1β, il6, tnfα and tgfβ, which can be regulated by IL10 and are promoters of liver fibrosis, were significantly lower than in the other groups. CONCLUSION: MSC administration is able to protect against liver fibrosis. intravenous injection is the most favorable treatment modality through promotion of IL10 expression.

关键词： Mesenchymal stem cells Hepatocyte differentiation intravenous injection Liver fibrosis Interleukin-10

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Acute kidney injury due to intravenous detergent poisoning:A case report

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World Journal of Clinical Cases 2022年第6期10卷 2036-2044页

作者： Sungbin Park Hyun-Sik Ryu Jae-Kwang Lee Sung-Soo Park Sun-Jung Kwon Won-Min Hwang Sung-Ro Yun Moon-Hyang Park Yohan Park Department of Internal Medicine Division of NephrologyKonyang University HospitalCollege of MedicineDaejeon 35365South Korea Department of Emergency Medicine Konyang University HospitalCollege of MedicineDaejeon 35365South Korea Department of Internal Medicine Division of Respiratory and Critical Care MedicineKonyang University HospitalCollege of MedicineDaejeon 35365South Korea Department of Pathology Konyang University HospitalCollege of MedicineDaejeon 35365South Korea

BACKGROUND Detergent poisoning mostly occurs through oral ingestion(>85%),ocular exposure(<15%),or dermal exposure(<8%).Reports of detergent poisoning through an intravenous injection are extremely *** addition,there ... 详细信息

BACKGROUND Detergent poisoning mostly occurs through oral ingestion(>85%),ocular exposure(intravenous injection are extremely *** addition,there are very few cases of renal toxicity directly caused by ***,we report a unique case of acute kidney injury caused by detergent poisoning through an accidental intravenous *** SUMMARY A 61-year-old man was intravenously injected with 20 mL of detergent by another patient in the same room of a local *** surfactant and calcium carbonate accounted for the largest proportion of the *** patient complained of vascular pain,chest discomfort,and nausea,and was transferred to our *** hospitalization,the patient’s serum creatinine level increased to 5.42 mg/dL,and his daily urine output decreased to approximately 300 *** biopsy findings noted that the glomeruli were relatively intact;however,diffuse acute tubular injury was *** edema was also noted,and the patient underwent a total of four hemodiafiltration ***,the patient’s urine output gradually increased whereas the serum creatinine level *** patient was discharged in a stable status without any *** Detergents appear to directly cause renal tubular injury by systemic *** treating a patient with detergent poisoning,physicians should be aware that the renal function may also *** addition,timely renal replacement therapy may help improve the patient’s prognosis.

关键词： Detergents Poisoning intravenous injection Acute kidney injury Acute tubular injury Case report

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intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?

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World Journal of Gastroenterology 2014年第48期20卷 18228-18239页

作者： Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz Centro de Pesquisa Experimental do Hospital de Clínicas de Porto Alegre Porto Alegre 90035-903Rio Grande do SulBrazil Programa de Pós-Gradua??o:Ciências em Gastroenterologia e Hepatologia Universidade Federal do Rio Grande do SulPorto Alegre 90035-903Rio Grande do SulBrazil Faculdade de Odontologia Universidade Federado Rio Grande do SulPorto Alegre 90035-003Rio Grande doSulBrazil

AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of ***:MSCs were isolated from C57BL/6 mouse adipose *** cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular *** acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to *** mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and *** disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and *** compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological *** responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 *** rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling ***:intravenous infusion of MSCs was more effective than intraperitoneal treatment(Pintravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in a

关键词： Ulcerative colitis Dextran sulfate sodium Inflammatory bowel disease Mesenchymal stem cell Cell transplantation intravenous injection Immunomodulation

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Comparison of the Concentrations of Lidocaine in Different Body Fluids/Tissues after Subarachnoid Space and intravenous Administration of a Lethal Dose of Lidocaine

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Journal of Forensic Science and Medicine 2015年第1期1卷 48-53页

作者： Nan Zhang JunHong Sun Zhiwen Wei Wenyan He Guohua Jin Xiaohong Zhang Pengxiang Gao Longmei Wang Keming Yun School of Forensic Medicine Shanxi Medical UniversityTaiyuanChina

The objective of the study was to compare the concentration of lidocaine in different body fluids/tissues after subarachnoid space and intravenous administrations of a lethal dose of *** 18 dogs were used in the *** dogs were given subarachnoid anesthesia,another were given an intravenous injection of a dose of 75 mg/kg weight of lidocaine hydrochloride in 5 min and the last 6 dogs were used as the blank control dogs and given a subarachnoid space injection or a femoral artery injection of the same volume of sodium *** soon as its vital signs disappeared,each dog was dissected and the specimen,such as brain,cerebrospinal fluid(CSF)in lateral ventricle,CSF in subarachnoid space,spinal cord(cervical spinal cord,thoracic spinal cord,lumbar spinal cord,and waist spinal cord),heart,lung,liver,spleen,kidney,bile,urine,heart blood,peripheral blood,muscle in injection location,and muscle in no injection location,were collected for analysis of lidocaine *** was performed with gas chromatography‑mass spectrometry(GC‑MS).From the maximum to the minimum,the order of lidocaine concentration detected in the subarachnoid space‑administered dogs was as follows:CSF in subarachnoid space,waist spinal cord,thoracic spinal cord,CSF in lateral ventricle,lumbar spinal cord,cervical spinal cord,lung,kidney,muscle in injection location,heart,brain,spleen,heart blood,liver,peripheral blood,bile,muscle in no injection location,and *** order of lidocaine concentration detected in the intravenously administered dogs was as followed:Kidney,heart,lung,spleen,brain,liver,peripheral blood,bile,heart blood,cervical spinal cord,thoracic spinal cord,muscle in injection location,lumbar spinal cord,muscle in no injection location,CSF in subarachnoid space,urine,and CSF in lateral *** maximum concentration of lidocaine was detected in the subarachnoid space CSF of subarachnoid space‑administered dead dogs,while in intravenously injected d

关键词： Anesthesia accident distribution of lidocaine forensic medicine forensic toxicological analysis intravenous administration intravenous injection lidocaine postmortem distribution subarachnoid administration subarachnoid anesthesia

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The ex vivo and in vivo biological performances of graphene oxide and the impact of surfactant on graphene oxide's biocompatibility

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Journal of Environmental Sciences 2013年第5期25卷 873-881页

作者： Guangbo Qu Xiaoyan Wang Qian Liu Rui Liu Nuoya Yin Juan Ma Liqun Chen Jiuyang He Sijin Liu Guibin Jiang State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of Sciences Department of Biomedical Engineering Tufts UniversityMedfordMassachusetts 02155USA

Graphene oxide（GO）displays promising properties for biomedical applications including drug delivery and cancer ***,GO exposure also raises safety concerns such as potential side effects on ***,the biological effects of GO suspended in phosphate buffered saline（PBS）with or without 1% nonionic surfactant Tween 80 were *** on the ex vivo experiments,Tween 80 significantly affected the interaction between GO and peripheral blood from *** suspension in PBS tended to provoke the aggregation of diluted blood cells,which could be prevented by the addition of Tween *** intravenous administration,GO suspension with or without 1% Tween 80 was quickly eliminated by the mononuclear phagocyte ***,GO suspension without Tween 80 showed greater accumulation in lungs than that containing 1% Tween *** contrast,less GO was found in livers for GO suspension compared to Tween 80 assisted GO *** including hearts,livers,lungs,spleens,kidneys,brains,and testes did not reveal histological *** indexes of peripheral blood showed no change upon GO *** results together demonstrated that Tween 80 could greatly alter GO＇s biological performance and determine the pattern of its biodistribution in mice.

关键词： graphene oxide biological performance nonionic surfactant Tween 80 intravenous injection

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Short-term intravenous interferon therapy for chronic hepatitis B

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World Journal of Gastroenterology 2008年第19期14卷 3038-3043页

作者： Hiroaki Okushin Toru Ohnishi Kazuhiko Morii Koichi Uesaka Shiro Yuasa Department of Internal Medicine Himeji Red Cross Hospital

AIM:To investigate the therapeutic efficacy of short- term, multiple daily dosing of intravenous interferon (IFN) in patients with hepatitis B e antigen (HBeAg)-positive chronic hepatitis B. METHODS:IFN-β was intravenously administered at a total dose of 102 million international units (MIU) over a period of 28 d in 26 patients positive for HBeAg and HBV-DNA. IFN-beta was administered at doses of 2 MIU and 1 MIU on d 1, 3 MIU twice daily from d 2 to d 7, and 1 MIU thrice daily from d 8 to d 28. Patients were followed up for 24 wk after the end of treatment. RESULTS:Six months after the end of the treatment, loss of HBV-DNA occurred in 13 (50.0%) of the 26 patients, loss of HBeAg in 9 (34.6%), development of anti-HBe in 10 (38.5%), HBeAg seroconversion in 8 (30.8%), and normalization of alanine aminotransferase (ALT) levels in 11 (42.0%). CONCLUSION:This 4-wk long IFN-β therapy, which was much shorter than conventional therapy lasting 12 wk or even more than 1 year, produced therapeutic effects similar to those achieved by IFN-α or pegylated- IFN-α (peg-IFN). Fewer adverse effects, greater efficacy, and a shorter treatment period led to an improvement in patients’ quality of life. IFN-β is administered intravenously, whereas IFN-α is administered intramuscularly or subcutaneously. Because both interferons are known to bind to an identical receptor and exert antiviral effects through intracellular signal transduction, the excellent results of IFN-β found in this study may be attributed to the multiple doses allowed by the intravenous route.

关键词： Chronic hepatitis B Hepatitis B e antigen Hepatitis B virus Interferon beta Multiple daily dosing Short-term treatment intravenous injection

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Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancer via ROS generation and microbiota modulation

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Acta Pharmaceutica Sinica B 2022年第2期12卷 907-923页

作者： Qiubing Chen Qian Li Yuqi Liang Menghang Zu Nanxi Chen Brandon S.B.Canup Liyong Luo Chenhui Wang Liang Zeng Bo Xiao State Key Laboratory of Silkworm Genome Biology College of SericultureTextileand Biomass SciencesSouthwest UniversityChongqing 400715China Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing School of Materials and EnergySouthwest UniversityChongqing 400715China Department of Chemistry Center for Diagnostics and TherapeuticsGeorgia State UniversityAtlantaGA 30303USA College of Food Science Southwest UniversityChongqing 400715China Chongqing Key Laboratory of Natural Product Synthesis and Drug Research School of Pharmaceutical SciencesChongqing UniversityChongqing 401331China

Although several artificial nanotherapeutics have been approved for practical treatment of metastatic breast cancer,their inefficient therapeutic outcomes,serious adverse effects,and high cost of mass production remain crucial ***,we developed an alternative strategy to specifically trigger apoptosis of breast tumors and inhibit their lung metastasis by using natural nanovehicles from tea flowers(TFENs).These nanovehicles had desirable particle sizes(131 nm),exosome-like morphology,and negative zeta ***,TFENs were found to contain large amounts of polyphenols,flavonoids,functional proteins,and *** experiments revealed that TFENs showed strong cytotoxicities against cancer cells due to the stimulation of reactive oxygen species(ROS)*** increased intracellular ROS amounts could not only trigger mitochondrial damage,but also arrest cell cycle,resulting in the in vitro anti-proliferation,anti-migration,and anti-invasion activities against breast cancer *** mice investigations demonstrated that TFENs after intravenous(i.v.)injection or oral administration could accumulate in breast tumors and lung metastatic sites,inhibit the growth and metastasis of breast cancer,and modulate gut *** study brings new insights to the green production of natural exosome-like nanoplatform for the inhibition of breast cancer and its lung metastasis via *** oral routes.

关键词： Tea flower Exosome-like nanoparticle Breast cancer Metastasis ROS generation Microbiota modulation intravenous injection Oral administration

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Metabolic utilization of intravenously injected iron from different iron sources in target tissues of broiler chickens

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Animal Nutrition 2022年第2期9卷 74-83页

作者： Lin Lu Xueyu Dong Xuelian Ma Liyang Zhang Sufen Li Xugang Luo Xiudong Liao Mineral Nutrition Research Division State Key Laboratory of Animal NutritionInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou225000China Department of Animal Science Hebei Normal University of Science and TechnologyQinhuangdao066004China

No information is available regarding the utilization of iron (Fe) from different Fe sources at a targettissue level. To detect differences in Fe metabolic utilization among Fe sources, the effect of intravenouslyinjected Fe on growth performance, hematological indices, tissue Fe concentrations and Fe-containingenzyme activities and gene expressions of Fe-containing enzymes or protein in broilers was *** d 22 post-hatching, a total of 432 male chickens were randomly allotted to 1 of 9 treatments ina completely randomized design. Chickens were injected with either a 0.9% (wt/vol) NaCl solution(control) or a 0.9% NaCl solution supplemented with Fe sulphate or 1 of 3 organic Fe sources. The 3organic Fe sources were Fe chelates with weak (Fe-MetW), moderate (Fe-ProtM) or extremely strong (Fe-ProtES) chelation strength. The 2 Fe dosages were calculated according to the Fe absorbabilities of 10%and 20% every 2 d for a duration of 20 d. Iron injection did not affect (P > 0.05) ADFI, ADG or FCR duringeither 1 to 10 d or 11 to 20 d after injections. Hematocrit and Fe concentrations in the liver and kidney ond 10 after Fe injections, and Fe concentrations in the liver or pancreas and ferritin heavy-chain (FTH1)protein expression level in the liver or spleen on d 20 after Fe injections increased (P≤0.05) as injectedFe dosages increased. When the injected Fe level was high at 20% Fe absorbability, the chickens injectedwith Fe-ProtES had lower (P injections. And they had lower (P injections than those injected with Fe-MetW or Fesulphate. The results from this study indicate that intravenously injected Fe from Fe-ProtES was the least utilizable and functioned in the sensitive target tissue less effectively than Fe from Fe sulfate, Fe-MetW or Fe-ProtM.

关键词： intravenous injection Iron-containing protein Gene expression Iron source Broiler

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Therapeutic efficacy of methylprednisolone sodium succinate via diverse administration routes for mid-to high-frequency sudden sensorineural hearing loss

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World Journal of Clinical Cases 2024年第18期12卷 3321-3331页

作者： Xiang-Da Meng Ting-Ting Li Li-Min Deng Department of Otolaryngology The First Affiliated Hospital of Qiqihar Medical UniversityQiqihar 161041Heilongjiang ProvinceChina Department of Otolaryngology Head and Neck Surgery Harbin Medical University Affiliated Fourth Hospital UniversityHarbin 150001Heilongjiang ProvinceChina Department of Facial Ward The First Affiliated Hospital of Qiqihar Medical UniversityQiqihar 161041Heilongjiang ProvinceChina

BACKGROUND Sudden sensorineural hearing loss(SSNHL),characterized by a rapid and unexplained loss of hearing,particularly at moderate to high frequencies,presents a significant clinical *** therapeutic use of methylprednisolone sodium succinate(MPSS)via different administration routes,in combination with conventional medications,remains a topic of *** To compare the therapeutic efficacy of MPSS administered via different routes in combination with conventional drugs for the treatment of mid-to high-frequency *** The medical records of 109 patients with mid-to high-frequency SSNHL were *** patients were divided into three groups based on the route of administration:Group A[intratympanic(IT)injection of MPSS combined with mecobalamin and Ginkgo biloba leaf extract injection],Group B(intravenous injection of MPSS combined with mecobalamin and Ginkgo biloba leaf extract injection),and Group C(single IT injection of MPSS).The intervention effects were compared and *** The posttreatment auditory thresholds in Group A(21.23±3.34)were significantly lower than those in Groups B(28.52±3.36)and C(30.23±4.21;Pinjection of MPSS combined with conventional treatment demonstrates superior efficacy and safety compared to systemic administ

关键词： Sudden loss of hearing at middle-and high-frequency of the descending type Methylprednisolone sodium succinate Intratympanic injection intravenous injection

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7-Ethyl-10-hydroxycamptothecin proliposomes with a novel preparation method: optimized formulation, characterization and in-vivo evaluation

7-Ethyl-10-hydroxycamptothecin proliposomes with a novel pre...

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第十届沈阳科学学术年会

作者： Shujun Wang Tiantian Ye Boyang Yang Xiaojun Yi Huimin Yao Department of Pharmaceutics School of Pharmacy Shenyang Pharmaceutical University Department of Pharmaceutics School of Chinese Medicines Shenyang Pharmaceutical University Department of Pharmaceutical and Food Science Tonghua Normal University

Context: The proliposomes were used to solve the stability of the ordinary liposomes. Objective: 7- ethyl- 10-hydroxycamptothecin （SN-38） proliposomes for intravenous （i.v.） administration were prepared successfully by a new method. Materials and methods: SN-38 liposomes solution was reconstituting automatically from proliposomes on contact with the acetic acid buffer solution （0.2 M, pH 2.6）. The formulation was optimized by the Box- Behnken design. The physicochemical characteristics of the SN-38 proliposomes were studied by scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, differential scanning calorimetry （DSC） and X-ray diffraction （XRD）. The stability studies were also carried on. The FLU-HPLC system was served to study the concentration of SN-38 in the plasma of Sprague Dawley （SD） rats. Results: The optimized formulation was SN-38: 0.03 g;Soybean phospholipid （SP）: 0.6 g;dextrose: 3.00 g. The entrapment efficiency of the optimized formulation was ＞85% and the mean particle size was about 231 nm. The stability studies showed that SN-38 proliposomes were stable in dark at 20-25℃ for 6 months at least. The pharmacokinetic parameters of i.v. administration demonstrated that the half-life of SN-38 loaded in the liposomes was prolonged in vivo. Discussion and conclusion: The SN-38 proliposomes was prepared successful by the analysis of TEM, SEM, DSC and XRD, and SN-38 liposomes could be reconstituted on contact with the hydration medium. SN-38 liposomes circulated for a longer time in the blood circulating system than SN-38 solution, which contributed to maintaining the drug action.

关键词： 7-ethyl-10-hydroxycamptothecin liposome proliposomes Box–Behnken design transmission electron microscopy differential scanning calorimetry stability intravenous injection pharmacokinetics

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