检索结果-南通市图书馆

维普期刊数据库

Recent Advances of Nanoelectrodes for Single-Cell Electroanalysis:From Extracellular, intercellular to Intracellular

在线全文

学校读者我要写书评

暂无评论

Journal of Analysis and Testing 2022年第2期6卷 178-192页

作者： Yu-Lin Liu Yu-Xiang Zhao Ya-Bei Li Zhao-Yang Ye Jun-Jie Zhang Yan Zhou Tian-Yang Gao Fei Li The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’an 710049China Bioinspired Engineering and Biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049China School of Chemistry Xi’an Jiaotong UniversityXi’an 710049China

Detection at single-cell level plays a critical role in revealing cell behavior in different organisms.Nanoelectrodes with high temporal–spatial resolution can precisely and dynamically monitor the physiological and pathological processes of various single cells.The field of using nanoelectrodes in single-cell electroanalysis is blooming in recent years.In this review,we mainly summarize the recent advances of nanoelectrodes for single-cell electroanalysis from extracellular,intercellular to intracellular levels in the past decade.First,we introduce the main types of nanoelectrodes based on their geometry and characteristics for single-cell electroanalysis.Then,the representative works of using nanoelectrodes to investigate cellular signaling biomolecules and to understand various cellular processes from the extracellular,intercellular,and intracellular levels are introduced.Finally,the challenges and future prospects of nanoelectrodes for single-cell electroanalysis are proposed.This review gives a comprehensive summary of nanoelectrodes for single-cell electroanalysis in the prospects of monitoring cell physiological topography,understanding communication mechanism and revealing physiological functions,which can provide new insights into cell-based pharmacological screening and fundamental studies of disease development mechanisms.

关键词： Nanoelectrode Single-cell electroanalysis Extracellular intercellular Intracellular

维普期刊数据库

Factors influencing intercellular spaces in the rat esophageal epithelium

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第9期16卷 1063-1069页

作者： Dong-Hong Zhang,Li-Ya Zhou,Xiu-Yun Dong,Rong-Li Cui,Yan Xue,San-Ren Lin,Department of Gastroenterology,Peking University Third Hospital,Beijing 100191,China Department of Gastroenterology Peking University Third Hospital No. 49 Huayuanbei Road Beijing 100191 China.

AIM:To evaluate the effect of acute stress,hydrochloric acid,ethanol,aspirin,and prednisolone on the intercellular spaces of the esophageal epithelium.METHODS:Part -RESULTS:(1) The f ive damaging factors produced no lesions or inflammation in esophageal mucosa of rats under either gross or routine histological inspections.Esophageal epithelial intercellular space diameters in stress and aspirin groups were significantly greater,nearly three or two-fold respectively,than those in their corresponding control groups (stress model:0.38 ± 0.05 μm vs 0.13 ± 0.02 μm,P 0.05;ethanol model:0.25 ± 0.10 μm vs 0.19 ± 0.05 μm,P > 0.05;prednisolone model:0.20 ± 0.03 μm vs 0.14 ± 0.03 μm,P > 0.05);and (2) No significant difference in the intercellular space diameters was observed between the group pretreated with esomeprazole and the control group,in both the stress and aspirin models (stress model:0.35 ± 0.05 μm vs 0.37 ± 0.05 μm,P > 0.05;aspirin model:0.24 ± 0.02 μm vs 0.27 ± 0.03 μm,P > 0.05).CONCLUSION:Acute stress and aspirin can induce DIS of the esophageal epithelium in rats,and it is not correlated with acid reflux.

关键词： Esophagus Dilated intercellular spaces Stress,acute Aspirin Reflux,acid

STUDY ON WAYS OF FORCING intercellular CYTOPLASM AND CHROMATIN MIGRATION TO TRANSFER EXTERNAL GENETIC SUBSTANCES TO PLANT CELLS BY ARTIFICIAL MOTIVE FORCE

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 1992年第3期35卷 291-303页

作者：吴伯骥陈毅平崔亚亚郑国锠杨庆兰 Chengdu Institute of Biology Academia Sinica Chengdu 610015 PRC Cytology Lab of Lanzhou University Lanzhou 730000 PRC

A new cell engineering technique (L. B. technique) was established in our Lab. At first, the physical and chemical methods were used to facilitate the reestablishment of intercellular contacts and plasmodesma channels between different parents, thus forcing cytoplasm and chromatin to pass the cell wall with different qualities and quantities from one cell to others, through the enlarged intercellular plasmodesma channels or the vulnerable regions and the holes on the cell wall formed differently in growth and thickness in the process of cell wall formation to introduce external genetic substances or gene groups into plant cells. There are different ways, frequencies and strengths for the migration between the cells in different growth and development regions or the same growth and development region. In this paper we advance the mechanism of cytoplasm and chromatin migration through the cell wall: There are a large number of plasmodesma channels or vulnerable regions and holes different in growth and

关键词： meristematic cell low osmotic pressure and low pH value K+ solution L. B. technique intercellular plasmodesma channel.

维普期刊数据库

intercellular MIGRATION OF NUCLEUS IN SOMATIC TISSUES

在线全文

学校读者我要写书评

暂无评论

中国科学A辑(英文版) 1959年第8期 853-871页

作者：吴素萱 Institute of Botany Academia Sinica

In this paper, a brief review is given of the recently published works dealing with the phenomenon of intercellular migration of the nuclei in reproductive and vegetative organs of the lower plants as well as the higher plants and also of the modern interpretations for such a phenomenon. The materials. employed in general for this study were the vegetative organs of bulb plants (Allium) and the somatic tissues of the ovules of some cereal plants (Triticum, Avena and Hordeum). The present report is chiefly based upon the studies of paraffin sections. Preparations of total mounts were also made for supplement. With the paraffin sections evidence is presented that the nuclear migration not only takes place in the epidermal tissues, in the underlying parenchymal tissues, but also occurs in the elements of the vascular bundles of the rapidly growing leaf sheath, young bud sheath, scale and clove sheath of the garlic plant. It is especially interesting to note that the nuclear material presses on the outer surface of the vessel and protrudes via the unthickened portions of the wall into the vessel. In the same material, enormous or extraordinarily long nuclei, which sometimes take a zigzag form, have usually been observed in the elements, including vessels, of the vascular bundles. These phenomena may have an important bearing on the mechanism of translocation of organic materials in plants-a view we have advanced earlier. As the leaf sheath or the young bud sheath begins to wither and its inclusion is being withdrawn, the intercellular migration of the nuclei again makes its appearance in the epidermis, underlying parenchyma and the vascular tissues. The nuclei, however, migrate in a different manner. They generally tend to traverse the side walls of the cells and move toward the vascular bundle.After fertilization, the antipodal cells of Triticurn, Avena and Hordeum plants multiply and increase a great deal in size. In the course of the development of endosperm sac, the cells of the nucellar tissue gradually diso

关键词： migration migrate sections sometimes nuclei sheath experienced inclusion intercellular Avena

Phase tissue intercellular adhesion molecule-1 expression in nude mice human liver cancer metastasis model

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 1998年第4期4卷 42-44页

作者： SUN JingJing, ZHOU XinDa, LIU YinKun and ZHOU GeKeywords liver neoplasms carcinoma, hepatocellular neoplasm metastasis intercellular adhesion molecule1 disease models, animal

Ｐｈａｓｅｔｉｓｓｕｅｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１ｅｘｐｒｅｓｓｉｏｎｉｎｎｕｄｅｍｉｃｅｈｕｍａｎｌｉｖｅｒｃａｎｃｅｒｍｅｔａｓｔａｓｉｓｍｏｄｅｌＳＵＮＪｉｎｇＪｉｎｇ，ＺＨ... 详细信息

Ｐｈａｓｅｔｉｓｓｕｅｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１ｅｘｐｒｅｓｓｉｏｎｉｎｎｕｄｅｍｉｃｅｈｕｍａｎｌｉｖｅｒｃａｎｃｅｒｍｅｔａｓｔａｓｉｓｍｏｄｅｌＳＵＮＪｉｎｇＪｉｎｇ，ＺＨＯＵＸｉｎＤａ，ＬＩＵＹｉｎＫ...

关键词： nude intercellular metastasis

Clinical significance of serum intercellular adhesion molecule-1 detection in patients with hepatocellular carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2000年第3期6卷 408-410页

作者： Ming Hui Mei Jing Xu Qin Fen Shi Jing Hong Yang Qian CHen Li Ling Qin Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery,Guilin Medical College,Guilin 541001,Guangxi Province,China 桂林医学院广西桂林

INTRODUCTION Since the late 1980s,studies on the expression ofintercellular adhesion molecule-1(ICAM-1)inpatients with malignancies have demonstrated thatICAM-1 may strongly express in two forms in suchdiseases:membra... 详细信息

关键词： carcinoma hepatocellular/diagnosis intercellular adhesion molecule-1 liverneoplasms/diagnosis alpha-fetoprotein

A clinical evaluation of serum concentrations of intercellular adhesion molecule-1 in patients with gastric cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 1998年第3期4卷 45-47页

作者： LIU Yong Zhong, CHEN Bin and SHE Xi Dian

Ａｃｌｉｎｉｃａｌｅｖａｌｕａｔｉｏｎｏｆｓｅｒｕｍｃｏｎｃｅｎｔｒａｔｉｏｎｓｏｆｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１ｉｎｐａｔｉｅｎｔｓｗｉｔｈｇａｓｔｒｉｃｃａｎｃｅｒＬＩＵＹｏｎｇＺ... 详细信息

Ａｃｌｉｎｉｃａｌｅｖａｌｕａｔｉｏｎｏｆｓｅｒｕｍｃｏｎｃｅｎｔｒａｔｉｏｎｓｏｆｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１ｉｎｐａｔｉｅｎｔｓｗｉｔｈｇａｓｔｒｉｃｃａｎｃｅｒＬＩＵＹｏｎｇＺｈｏｎｇ，ＣＨＥＮＢｉｎａｎｄＳＨ...

关键词： stomach neoplasms/immunology intercellular adhesion molecule 1/blood lymphatic metastasis intercellular adhesion molecule 1/analysis enzyme linked immunosorbent assay

Expression of intercellular adhesive molecule-1 in liver cancer tissues and liver cancer metastasis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 1998年第3期4卷 22-25页

作者： SUN Jing Jing, ZHOU Xin Da, ZHOU Ge and LIU Yin Kun

Ｅｘｐｒｅｓｓｉｏｎｏｆｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｖｅｍｏｌｅｃｕｌｅ１ｉｎｌｉｖｅｒｃａｎｃｅｒｔｉｓｓｕｅｓａｎｄｌｉｖｅｒｃａｎｃｅｒｍｅｔａｓｔａｓｉｓＳＵＮＪｉｎｇＪｉｎｇ，ＺＨＯＵＸｉｎＤ... 详细信息

Ｅｘｐｒｅｓｓｉｏｎｏｆｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｖｅｍｏｌｅｃｕｌｅ１ｉｎｌｉｖｅｒｃａｎｃｅｒｔｉｓｓｕｅｓａｎｄｌｉｖｅｒｃａｎｃｅｒｍｅｔａｓｔａｓｉｓＳＵＮＪｉｎｇＪｉｎｇ，ＺＨＯＵＸｉｎＤａ，ＺＨＯＵＧｅａｎｄＬＩＵＹｉｎ...

关键词： liver cancer tumor metastasis intercellular adhesion molecule 1

Enterocyte dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin expression in inflammatory bowel disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第1期21卷 187-195页

作者： Jing-Qing Zeng Chun-Di Xu Tong Zhou Jing Wu Kai Lin Wei Liu Xin-Qiong Wang Department of Pediatrics Ruijin HospitalShanghai Jiao Tong University School of Medicine Department of Pediatrics Shanghai Children’s Medical CenterShanghai Jiao Tong University School of Medicine

AIM: To investigate dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin(DC-SIGN) expression in intestinal epithelial cells(IECs) in inflammatory bowel disease(IBD).METHODS: The expression of DC-SIGN in IECs was examined by immunohistochemistry of intestinal mucosal biopsies from 32 patients with IBD and 10 controls.Disease activity indices and histopathology scores were used to assess the tissue lesions and pathologic damage.Animal studies utilized BALB/c mice with dextran sodium sulfate(DSS)-induced colitis treated with anti-P-selectin lectin-EGF domain monoclonal antibody(PsL-EGFmA b).Controls,untreated and treated mice were sacrificed after 7 d,followed by isolation of colon tissue and IECs.Colonic expression of DC-SIGN,CD80,CD86 and MHC Ⅱ was examined by immunohistochemistry or flow cytometry.The capacity of mouse enterocytes or dendritic cells to activate T cells was determined by coculture with naive CD4+ T cells.Culture supernatant and intracellular levels of interleukin(IL)-4 and interferon(IFN)-γ were measured by enzyme-linked immunosorbent assay and flow cytometry,respectively.The ability of IECs to promote T cell proliferation was detected by flow cytometry staining with carboxyfluorescein diacetate succinimidyl ester.RESULTS: Compared with controls,DC-SIGN expression was significantly increased in IECs from patients with Crohn's disease(P < 0.01) or ulcerative colitis(P < 0.05).DC-SIGN expression was strongly correlated with disease severity in IBD(r = 0.48; P < 0.05).Similarly,in the DSS-induced colitis mouse model,IECs showed upregulated expression of DC-SIGN,CD80,CD86 and MHC,and DC-SIGN expression was positively correlated with disease activity(r = 0.62: P < 0.01).IECs from mouse colitis stimulated naive T cells to generate IL-4(P < 0.05).Otherwise,dendritic cells promoted a T-helper-1-skewing phenotype by stimulating IFN-γ secretion.However,DC-SIGN expression and T cell differentiation were suppressed following treatment of mice with DSS-induced colitis with Ps L-EGFm

关键词： Dendritic cell-specific intercellular adhesion mol