Enterocyte dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin expression in inflammatory bowel disease

作     者：Jing-Qing Zeng Chun-Di Xu Tong Zhou Jing Wu Kai Lin Wei Liu Xin-Qiong Wang 

作者机构：Department of PediatricsRuijin HospitalShanghai Jiao Tong University School of Medicine Department of PediatricsShanghai Children’s Medical CenterShanghai Jiao Tong University School of Medicine 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2015年第21卷第1期

页      面：187-195页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Grants from the National Natural Science Foundation of China No.81000163 No.81070567 and No.81170363 

主　　题：Dendritic cell-specific intercellular adhesion mol 

摘      要：AIM: To investigate dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin(DC-SIGN) expression in intestinal epithelial cells(IECs) in inflammatory bowel disease(IBD).METHODS: The expression of DC-SIGN in IECs was examined by immunohistochemistry of intestinal mucosal biopsies from 32 patients with IBD and 10 *** activity indices and histopathology scores were used to assess the tissue lesions and pathologic *** studies utilized BALB/c mice with dextran sodium sulfate(DSS)-induced colitis treated with anti-P-selectin lectin-EGF domain monoclonal antibody(PsL-EGFmA b).Controls,untreated and treated mice were sacrificed after 7 d,followed by isolation of colon tissue and *** expression of DC-SIGN,CD80,CD86 and MHC Ⅱ was examined by immunohistochemistry or flow *** capacity of mouse enterocytes or dendritic cells to activate T cells was determined by coculture with naive CD4+ T *** supernatant and intracellular levels of interleukin(IL)-4 and interferon(IFN)-γ were measured by enzyme-linked immunosorbent assay and flow cytometry,*** ability of IECs to promote T cell proliferation was detected by flow cytometry staining with carboxyfluorescein diacetate succinimidyl ***: Compared with controls,DC-SIGN expression was significantly increased in IECs from patients with Crohn s disease(P 0.01) or ulcerative colitis(P 0.05).DC-SIGN expression was strongly correlated with disease severity in IBD(r = 0.48; P 0.05).Similarly,in the DSS-induced colitis mouse model,IECs showed upregulated expression of DC-SIGN,CD80,CD86 and MHC,and DC-SIGN expression was positively correlated with disease activity(r = 0.62: P 0.01).IECs from mouse colitis stimulated naive T cells to generate IL-4(P 0.05).Otherwise,dendritic cells promoted a T-helper-1-skewing phenotype by stimulating IFN-γ ***,DC-SIGN expression and T cell differentiation were suppressed following treatment