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Molecular Characterization, Expression Pattern and Transcriptional Regulation of Figla During gonad development in Japanese Founder (Paralichthys olivaceus)

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Journal of Ocean University of China 2022年第4期21卷 1037-1050页

作者： QU Jiangbo LI Rui LIU Yuxiang SUN Minmin YAN Weijie LIU Jinxiang WANG Xubo ZHANG Quanqi MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for MarineScience and TechnologyQingdao 266237China

The factor in the germline alpha(figla), as a member of the basic helix-loop-helix family, has been reported to be involved in ovary development in mammals and teleosts. However, the regulatory mechanisms of figla in teleosts remain unclear. Here,figla in P. olivaceus(Pofigla) was characterized with encoding a 202 amino acid protein that contains a conserved basic region and helix-loop-helix(HLH) domain. Amino acids alignment and synteny analysis revealed that Pofigla was conserved with the orthologous gene sequences in other vertebrates. The results of qRT-PCR showed Pofigla was maternally inherited during embryonic development. For tissue distribution, Pofigla showed a sexually dimorphic gene expression in the gonad of different genders, with a higher expression in ovary than in testis. In situ hybridization(ISH) results demonstrated Pofigla was specifically expressed in germ cells including oocytes, spermatogonia and spermatocytes. By screening and analyzing two proximal regions(-2966/-2126 and-772/-444) with high promoter activity, we found SOX5, LEF1, FOXP1 and GATA1 may play important roles in the transcriptional regulation of Pofigla. Furthermore, we observed the co-localization between Figla and LEF1 in HEK 293T cells. And the significant up-regulation effect of the canonical Wnt signaling pathway on the expression of Pofigla was found in cultured ovarian cells. This study provided the first evidence that figla not only has an important function in ovary development, but also plays some potential roles in testis development and/or male germ cell differentiation during early testis development in P. olivaceus. The results provide valuable reference in exploring the regulatory network of figla in teleost.

关键词： figla transcriptional regulation Wnt signaling pathway gonad development Paralichthys olivaceus

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Comparative Study of the Growth and gonad development of Clarias gariepinus （Burchell 1822） Fed Diets with Plant and Animal-based Ingredients in Concrete Tanks

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Journal of Agricultural Science and Technology(A) 2012年第10期2卷 1203-1210页

作者： Albert Philip Ekanem Sunday Urom Eteng Francis Maduwuba Nwosu Victor Oscar Eyo Institute of Oceanography University of Calabar PMB 111 5 Calabar Nigeria Department of Zoology and Environmental Biology University of Calabar PMB 1115 Calabar Nigeria

A twelve week experiment was conducted to compare the effects of diets with plant-based and animal-based ingredients on growth and gonad development of Clarias gariepinus. One hundred and 12 sub-adult C. gariepinus with an average weight of 205 ×5.09 g, were stocked in six concrete tanks （9 × 4 × 2 m3） containing 20 fish each. Experiment had 3 replicates and animals fed daily at 5% body weights. There was no significant difference （P 〈 0.05） in proximate compositions of the two experimental feeds and controls. Fecundity, gonad weight and gonasomatic index were higher in fish fed diet 13 than A and C. Similarly, growth indices were higher in fish fed diet B than in A and C. Histology of gonads showed a faster development of oocytes of eggs in fish fed animal-based ingredients than plant-based and combined plant and animal diets. Although there were slight differences in growth parameters and gonad development in favor of feed with animal-based ingredients, plant-based feed compared favorably in the growth and gonad development of C. gariepinus. Plant-based ingredients are recommended on the basis of affordability and availability as substitute for animal-based ingredients in C. gariepinus feed.

关键词： Plant-based animal-based diets growth gonad development Clarias gariepinus.

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Effect of temperature and photoperiod on maturation of Cephalothrix hongkongiensis(Nemertea: Palaeonemertea)

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Chinese Journal of Oceanology and Limnology 2014年第4期32卷 764-772页

作者：李应东宋肖跃张珊珊朱林孙世春 Institute of Evolution and Marine Biodiversity Ocean University of China

The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few species have been studied under well-controlled laboratory conditions. In this work, the effects of temperature and photoperiod on gonad development and spawning of Cephalothrix hongkongiensis were observed by rearing worms under different temperatures and photoperiods. Worms reared at 4~C and 6~C showed no growth or gonadal development, and all died within 109 days. At 8~C and 12~C, gonads could be seen from 90 days to the termination of the 360-day experiment, but no spawning was observed. Worms maintained at 16, 24, and 28~C showed both gonadal development and spawning, and matured faster at higher temperatures. Under a fixed temperature （16℃）, photoperiod did not have any significant impact on maturation and spawning. It is likely that temperature plays a major role in regulating the maturation and reproductive cycle of C. hongkongiensis.

关键词： temperature photoperiod gonad development reproduction Cephalothrix hongkongiensis Nemertea

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gonad traits and age structure of the sea urchin Heliocidaris crassispina in central range:a comparison with rangeextenders

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Journal of Oceanology and Limnology 2023年第5期41卷 1954-1964页

作者： Wenping FENG Daichi NAKANO Nobuyasu NAKABAYASHI Masakazu NAOKI Xiaofei TIAN Yukio AGATSUMA Fisheries College Zhejiang Ocean UniversityZhoushan 316022China Graduate School of Agricultural Science Tohoku UniversitySendai 980-8572Japan Fukui Prefectural University Fukui 910-1195Japan Akita Prefectural Institute of Fisheries Oga 010-8570Japan

The sea urchin Heliocidaris crassispina has northern expanded in geographic range to Toga Bay,Oga Peninsula in the Sea of Japan due to the global *** demonstrate the gonad traits and age structure of *** in the central range and compare the difference with those in northern extended range,we collected 100 *** individuals from a barren off Shitsumi,Fukui(central range),in August *** growth,gonad traits(size,development and color),and sex ratio were investigated and compared with those in Toga Bay,in August 2014(extended range).The successive 2012–2016 year classes of *** indicated successive juvenile recruitment in the central range,as found in the extended *** individuals at three years old in central range were obviously larger than those in the extended range,showing large test diameter and body weight,possibly due to the higher temperature in central range than that in northern extended *** addition,a balanced sex ratio in the central range was found,in contrast to the female-skewed sex ratio found in the extended *** development was sex-and *** testicular development in the central range was not only delayed in comparison to ovarian development,but also less delayed than those in the extended range,which possibly attribute to the higher water temperature in the central range.A significantly lower male gonad index and L*(lightness)value was recorded in central ranges compared to that in extended range,possibly due to the releasement of sperm of *** in central ranges.

关键词： Heliocidaris crassispina age gonad development gonad color range extension

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Interspecific Hybridization Between Crassostrea angulata and C. ariakensis

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Journal of Ocean University of China 2015年第4期14卷 710-716页

作者： YAO Tuo ZHANG Yuehuan YAN Xiwu WANG Zhaoping LI Dongchun SU Jiaqi YU Ruihai Fisheries College Ocean University of China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences Engineering Research Center of Shellfish Culture and Breeding of Liaoning Province College of Fisheries and Life Science Dalian Ocean University

lnterspecific hybridization can generate heterosis, which is proven to be a useful tool in selective breeding programs for oyster culture. Crassostrea angulata and C. ariakensis are two important economic shellfish species in China. We conducted 2 × 2 reciprocal crosses to determine whether these two species can cross-fertilize and their hybrids can hatch, survive and perform hetero- sis. Fertilization was found symmetrical without delay. The rate of fertilization success of C. angulata ♀× C. ariakensis ♂ was lower than that of C. ariakensis ♀ x C. angulata ♂, and the success rate of both hybridizations was lower than that of two intraspecific crosses each. During the planktonic period, survival rate of the progeny was lower in the hybrid crosses than in the intraspecific crosses. On day 360, mean shell height of the progeny of C. angulata ♀ x C. angulata ♂ was highest, which was followed by that of C. angulata ♀ × C. ariakensis ♂, C. ariakensis ♀x C. ariakensis ♂ and C. ariakensis ♀× C. angulata ♂ in a descending order. Mor- phology of adults produced by the hybrid crosses was similar to that of C. angulata. Both hybrids underwent normal gonad devel- opment and produced mature gametes in the mating season. This study provided new insights into the quantitative traits in interspeci- fic crosses of Crassostrea species, thus being of guidance value for selective breeding of oyster.

关键词： Crassostrea angulata Crassostrea ariakensis interspecific hybridization gonad development heterosis

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Characterization and expression analysis of sox3 in medaka gonads

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Aquaculture and Fisheries 2022年第1期7卷 23-30页

作者： Quan Pu Yuan Ma Ying Zhong Jing Guo Lang Gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

Numerous studies have showed that sox3 is involved in neurogenesis and sex differentiation in ***,the accurate expression pattern is still unclear in *** this study,medaka sox3 was isolated and its expression patterns were compared with germ cell gene vasa in adult *** reverse transcription-polymerase chain reaction(RT-PCR)analysis,sox3 RNA expression was detected in the brain,eyes and *** chromogenic and fluorescent in situ hybridization,sox3 was present in the oogonia and early stages of oocytes as well as granulosa cells and theca cells in the later stages;in the testis,sox3 was occurred in the Sertoli cells and sperm with a specific signal in the location of chromatid body of sperm;in the embryos,sox3 was expressed in the central *** results suggest that medaka sox3 gene was occurred in somatic and germ cells in adult gonads of both sexes,involved in the process of spermatogenesis,as well as the development of the nervous *** study provides a precise expression pattern of sox3 and suggests that sox3 may be involved in gonadal and nervous system development of fish.

关键词： Medaka sox3 gonad development Nervous system development

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Variation of lipids and fatty acids composition in the tissues of wild devil stinger(Inimicus japonicas)during sexual maturation

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Aquaculture and Fisheries 2018年第3期3卷 115-121页

作者： Songlin Li Wen Wen Xu Gong Xuxiong Huang Naisong Chen National Demonstration Center on Experiment Teaching of Fisheries Science Shanghai Ocean UniversityShanghai 201306China Research Centre of the Agriculture Ministry on Environmental Ecology and Fish Nutrition Shanghai Ocean UniversityShanghai 20136China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai 201306China

The present study was conducted to characterize the lipid and fatty acid profiles in different tissues(gonad,liver and muscle)of the wild-caught devil stinger during sexual ***-caught devil stinger were divided into six groups according to gonad development and *** lipid content was up to 40%dry weight in liver and was significantly higher than in other tissues(ovary,testis and muscle)in both male and female stinger during sexual *** maturation,total lipid content in the female stinger liver decreased,but in males significantly increased in all *** proportions of neutral lipids(NL)were observed in the stinger liver,while polar lipids(PL)were mainly quantified in the gonad and muscle of both male and female *** PL content of the ovary significantly decreased with sexual maturation,but NL were significantly *** fatty acids composition of the stinger broodstocks were tissue-and lipid *** the stinger gonad high-unsaturated fatty acids(HUFA),such as ARA,DHA and EPA,are selectively transferred and conserved,indicating the crucial role of these fatty acids during sexual maturation of this fish species.

关键词： Lipids Fatty acids composition gonad development Devil stinger

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Study on Early Propagation Technology of Yellow Catfish (Pelteobagrus fulvidraco)

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Agricultural Biotechnology 2020年第1期9卷 63-66,69页

作者： Heng ZHANG Guoliang RUAN Kun GUO Wenhao FAN Weiyou ZHENG Engineering Research Center of Ecology and Agricultural Use of Wetland Ministry of EducationCollege of Animal ScienceYangtze UniversityJingzhou 434025China Hubei Research Institute of Aquatic Industry Technology Jingzhou 434000China

[Objectives] This study was conducted to study early reproduction techniques of yellow catfish(Pelteobagrus fulvidraco) in Jianghan Plain. [Methods] Parents of P. fulvidraco were cultured by the methods of artificial temperature control and nutrition enhancement. [Results] After culture, the body weight, gonad weight, maturity coefficient and egg diameter of P. fulvidraco increased gradually. Up to April 30, the gonad weight of the female parents of P. fulvidraco cultured in greenhouses was significantly higher than that of the parents under natural conditions(Pgonad maturity of P. fulvidraco cultured under strengthened conditions was 15 d earlier than that under natural conditions, resulting in mass production of early-propagated larvae of P. fulvidraco. [Conclusions] In Jianghan plain area, artificial propagation can be achieved in early May by means of artificial temperature control and strengthened culture, which is 20 d ahead of natural conditions.

关键词： Pelteobagrus fulvidraco Early propagation gonad development

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A Novel igf3 Gene in Common Carp（Cyprinus carpio）:Evidence for Its Role in Regulating gonadal development

A Novel igf3 Gene in Common Carp（Cyprinus carpio）:Evidence...

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江苏省遗传学会2016年学术年会

作者：宋飞彪 Lanmei Wang Wenbin Zhu Jianjun Fu Juanjuan Dong 董在杰 Wuxi Fisheries College Nanjing Agricultural University Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry Of AgricultureFreshwater Fisheries Research CenterChinese Academy of Fishery Sciences

Since the insulin-like growth factor 3（igf3） gene was recently discovered in fish ovary,its function in the gonads has received much *** this study,we isolated two igf3 subtypes from common carp（Cyprinus carpio）,which comprised full-length c DNA of 707 and 1153 nucleotides encoding 205 and 198 amino acids（aa）,*** Igf-3 aa sequence had the highest gene homology of 72% with the corresponding sequence in zebrafish（Danio rerio）.Phylogenetic tree construction revealed that the ***3 gene was first clustered with *** and then with other teleost ***3 mRNA was widely expressed,with expression being highest in the gonads and *** the gonad development stage,igf3 mRNA expression was highest in the recession and fully mature periods of the ovaries and testes,*** blotting of testis protein samples showed two bands of approximately 21 kDa and 34 kDa corresponding to the calculated molecular mass of the two Igf-3 *** Igf-3 protein was localized in the ovary granulosa cells and testis interstitial cells.17β-Ethinylestradiol treatment increased both ovary and testis igf3 mRNA *** findings suggest that Igf-3 may play an important role in *** gonadal development.

关键词： Igf-3 cDNA cloning expression 17β-ethinylestradiol(E2) gonad development Cyprinus carpio

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