A Novel igf3 Gene in Common Carp（Cyprinus carpio）:Evidence for Its Role in Regulating Gonadal Development
作者单位：Wuxi Fisheries CollegeNanjing Agricultural University Key Laboratory of Freshwater Fisheries and Germplasm Resources UtilizationMinistry Of AgricultureFreshwater Fisheries Research CenterChinese Academy of Fishery Sciences
会议名称：《江苏省遗传学会2016年学术年会》
会议日期：2016年
基 金：中央级公益性科研院所基本科研业务费专项资金项目(2015JBFR01) 国家现代农业产业技术体系建设专项资金项目(CARS-46) “十二五”国家科技支撑计划项目(2012BAD26B02)
关 键 词：Igf-3 cDNA cloning expression 17β-ethinylestradiol(E2) gonad development Cyprinus carpio
摘 要：Since the insulin-like growth factor 3（igf3） gene was recently discovered in fish ovary,its function in the gonads has received much *** this study,we isolated two igf3 subtypes from common carp（Cyprinus carpio）,which comprised full-length c DNA of 707 and 1153 nucleotides encoding 205 and 198 amino acids（aa）,*** Igf-3 aa sequence had the highest gene homology of 72% with the corresponding sequence in zebrafish（Danio rerio）.Phylogenetic tree construction revealed that the ***3 gene was first clustered with *** and then with other teleost ***3 mRNA was widely expressed,with expression being highest in the gonads and *** the gonad development stage,igf3 mRNA expression was highest in the recession and fully mature periods of the ovaries and testes,*** blotting of testis protein samples showed two bands of approximately 21 kDa and 34 kDa corresponding to the calculated molecular mass of the two Igf-3 *** Igf-3 protein was localized in the ovary granulosa cells and testis interstitial cells.17β-Ethinylestradiol treatment increased both ovary and testis igf3 mRNA *** findings suggest that Igf-3 may play an important role in *** gonadal development.