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glyco-poly-l-lysine is better than liposomal delivery of exogenous genes to rat liver

World Journal of Gastroenterology 2000年第4期6卷 526-531页

作者： Yang, CQ Wang, JY He, BM liu, JJ Guo, JS Shanghai Med Univ Zhongshan Hosp Div Gastroenterol Shanghai 200032 Peoples R China

AIM To compare the effects of liposomes andglyco-poly-l-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-l-lysine,theplasmid could be expressed in eukaryotic cellswhen injected into Wistar rats by intravenousroute.At different time intervals after the injection,the distribution and expression of the plasmid inliver of rats were observed and compared using insitu hybridization and immunohistochemistry.RESUlTS The expression of the plasmid bindingto liposomes or G-Pll could be markedly observed24 h later,and began to decrease one week later,but it still could be observed up to three weeks.Both liposomes and G-Pll could deliver theplasmid to the liver effectively,but the effect of thelatter was better than the former concerning thedistribution and expression of the plasmid targeteduptake in the liver.CONClUSION G-Pll is better than liposome asthe targeted carrier for delivering exogenous genesto the liver.

关键词： liposomes glyco-poly-l-lysine targeted liver uptake exogenous gene

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A comparison between intravenous and peritoneal route on liver targeted uptake and expression of plasmid delivered by glyco-poly-l-lysine

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World Journal of Gastroenterology 2000年第4期6卷 508-512页

作者： Yang CQ Wang JY Fang GT liu JJ Guo JS Shanghai Med Univ Zhongshan Hosp Div Gastroenterol Shanghai 200032 Peoples R China

AIM To compare the effects of intravenous routeand peritoneal route on liver targeted uptake andexpression of plasmid delivered by galactose-terminal glyco-poly-l-lysine(G-Pll).METHODS The plasmid pTM/MMP-1 which couldbe expressed in eukaryotic cells was bound to G-Pll,and was then transferred into Wistar rats byintravenous and intraperitoneal injection.Theexpression and distribution of the plasmid wereobserved at different time periods by in situhybridization and immunohistochemistry.RESUlTS The plasmid could be expressedsignificantly within 24 h after being transferred invivo by both intravenous and intraperitonealroutes.One week later the expression began todecrease,and could still be observed three weekslater.Although both the intravenous andintraperitoneal route could target-specificallydeliver the plasmid to the liver,the effect of theformer was better as compared to that of the latter.CONClUSION Intravenous route is better for livertargeted uptake and expression of G-Pll-boundplasmids than the peritoneal route.

关键词： intravenous injection intraperitoneal injection glyco-poly-l-lysine lwcr targeted uptake

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