A comparison between intravenous and peritoneal route on liver targeted uptake and expression of plasmid delivered by Glyco-poly-L-lysine
A comparison between intravenous and peritoneal route on liver targeted uptake and expression of plasmid delivered by Glyco-poly-L-lysine作者机构：Shanghai Med Univ Zhongshan Hosp Div Gastroenterol Shanghai 200032 Peoples R China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2000年第6卷第4期
页 面：508-512页
核心收录：
基 金：National Natural Science Foundation of China(№39570336).
主 题：intravenous injection intraperitoneal injection glyco-poly-L-lysine lwcr targeted uptake
摘 要：AIM To compare the effects of intravenous routeand peritoneal route on liver targeted uptake andexpression of plasmid delivered by galactose-terminal glyco-poly-L-lysine(G-PLL).METHODS The plasmid pTM/MMP-1 which couldbe expressed in eukaryotic cells was bound to G-PLL,and was then transferred into Wistar rats byintravenous and intraperitoneal injection.Theexpression and distribution of the plasmid wereobserved at different time periods by in situhybridization and immunohistochemistry.RESULTS The plasmid could be expressedsignificantly within 24 h after being transferred invivo by both intravenous and intraperitonealroutes.One week later the expression began todecrease,and could still be observed three weekslater.Although both the intravenous andintraperitoneal route could target-specificallydeliver the plasmid to the liver,the effect of theformer was better as compared to that of the latter.CONCLUSION Intravenous route is better for livertargeted uptake and expression of G-PLL-boundplasmids than the peritoneal route.
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