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High glucose changes mitochondrial dynamics in cultured human glomerular endothelial cells

中国药理学与毒理学杂志 2019年第10期 927-928页

作者： YU Bai-xue SHI Yi Biomedical Research Center Zhongshan Hospital Fudan University

OBJECTIVE To investigate the effects of high glucose on mitochondrial dynamic changes in cultured human glomerular endothelial cells（HGECs）.METHODS HGECs were subjected to high glucose(44.4 mmol·L;) or mannitol for six-hour or *** were collected for real-time polymerase chain reactions. RESULTS After six hours, mRNA expression of mitochondrial fusion protein mitofusion（MFN）-2, but not MFN1, was increased in HGEC stimulated with high glucose. It suggests that exchanges of intramitochondrial material are enhanced. Message RNA expression of mitochondrial fission factor（MFF） was increased as well,indicating an increase in mitochondrial fragmentation,probably leading to mitophagy. Nevertheless, the enhanced mitochondrial dynamic changes were not observed after 24 h stimulation. Thus, the transient mitochondrial dynamic changes indicate an adaptive mechanism in HEGC challenging with high glucose. CONCLUSION High glucose transiently alters mitochondrial dynamic genes expression in HGEC. Further experiments will be designed to investigate underlying mechanisms in HGEC as well as in glomerular endothelial cells of diabetic nephropathy.

关键词： high glucose mitochondrial dynamics glomerular endothelial cells

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The Effect of Endoplasmic Reticulum Stress Protein CHOP on Cyclosporine A-induced Injury of glomerular endothelial cells

The Effect of Endoplasmic Reticulum Stress Protein CHOP on C...

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第八届海峡两岸心血管科学研讨会

作者： The ZHOU~(1,2),Yi WU~(1,2),Xiang MENG~1,Dan MENG~1, Li WANG~1,Si-feng CHEN~(1*),Wen-jun YUAN~(2*) (1.Departent of Physiology and Pathophysiology,Fudan University Shanghai Medical College, Shanghai,China 2.Departent of Physiology,Ningxia Medical University,Xining,China)

＜正＞Objective Cyclosporine A（CsA） is one of the most commonly used immunosuppressive reagents, the major clinical drugs for anti - rejection response after renal *** preventing allograft immune rejection,CsA may injure many cells including epithelial renal ***,if CsA can cause injury and dysfunction in glomerular endothelial cells（GECs）,a major mechanism of transplant loss,is unknown. This study aimed to study the role of C/EBP homologous protein（CHOP） in the CsA induced injury of GECs. Materials and Methods Primary GECs were isolated by cell adherent and growth selection assay.25μmol/ L CsA was used to induce cell injury and endoplasmic reticulum stress response（ER） of the *** expression of CHOP was inhibited by *** change of protein was evaluated by Western *** apoptosis, proliferation and migration were measured by annexin - V and PI double staining,MTT degradation assay and cell monolayer scratch assays,*** After treatment of GECs with CsA（25μmol/ L） for 8 hours,proliferation and migration of the cells decreased while CHOP expression and apoptosis increased as compared to cells without CsA *** - siRNA inhibited CsA - induced CHOP expression and decreased apoptosis rate of the cells to normal level while proliferation and migration activities of the cells remained *** expression of CHOP’ s upstream protein ATF6 and activated ATF6 was not significantly affected by CsA and CHOP - *** Pharmacological dose of CsA inhibited cell functions and induced cell apoptosis of *** expression of CHOP was one of the major mechanism of CsA - induced cell *** results not only revealed a new mechanism of CsA - induced renal toxicity but may also provide a new idea to prolong graft survival time.

关键词： cyclosporin A CHOP glomerular endothelial cells endoplasmic reticulum stress ATF-6

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Carbon Monoxide Prevents Cyclosporine A-induced Injury of glomerular endothelial cells

Carbon Monoxide Prevents Cyclosporine A-induced Injury of Gl...

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第八届海峡两岸心血管科学研讨会

作者： Zhe Zhou~(1,2),Yi Wu~(1,2),Meng Xiang~1,Dan Meng~1,Li Wang~1, Si-feng Chen~(1*),Wen-jun Yuan~(2*) (1.Departent of Physiology and Pathophysiology,Fudan University Shanghai Medical College, Shanghai,China 2.Departent of Physiology,Ningxia Medical University,Yinchuan,China)

＜正＞Objective Most kidney allografts eventually lose their function because of chronic *** A（CsA） is one of the most commonly used immunosuppressive *** preventing allograft immune rejection,CsA may injure many renal *** oxygenase-1（HO-1） is protective for numerous cells including renal epithelial cells by presenting anti-inflammatory,anti-oxidative and anti-apoptotic properties through its catalyzed products such;as carbon monoxide（CO）.However,the effects of CsA on glomerular endothelial cells（GECs） and the role of Ho-1 arenot *** study observed the effects of HO -1 and CO on CsA-induced GEC injury and *** and Methods Primary GECs were treated with 25μmol/L CsA to induce injury and *** and CORM -2 were used for representatives for HO-1 inducers and HO-1 catalyzed products,*** expressions of HO-1 and endoplasmic reticulum stress response（ER） proteins（CHOP and ATF -6） were evaluated by Western bolting. Cell apoptosis,proliferation and migration were measured by annexin-V and PI double staining,MTT degradation assay and cell monolayer scratch assays,*** At 8 h after treatment,HO-1 and CHOP expression in CsA-treated cells increased significantly compared to untreated *** promoted apoptosis and inhibited cell proliferation and migration of the *** increased HO-1 protein and mRNA expressions *** hemin decreased CsA -induced CHOP expression,it did not prevent CsA -induced apoptosis,proliferation and migration inhibitions compared to untreated ***-releasing molecule CORM-2 did not affect biological properties and protein expressions of the cells without CsA treatment although it decreased migration of the ***,it blocked CsAinduced cell proliferation inhibition, apoptosis and CHOP expression although it did not change cell migration,ATF-6 expression and activation of CsA-treated *** Pharmacological dose of Cs

关键词： cyclosporin A heme oxygenase-1 carbon monoxide CORM -2 glomerular endothelial cells endoplasmic reticulum stress

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Effects of Maternal Serum on Permeability of glomerular endothelial Cell Membrane

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第1期31卷 17-20页

作者：杜慧刘海意赵君吴媛媛龚洵周琼徐晶晶李宇琪石鑫玮乔福元 Department of Gynaecology and Obstetrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

The mechanism of injury on the human glomerular endothelial cells （ciGENC） induced by preeclampsia serum was investigated. Concentration of maternal serum sFlt-1 protein was detected by ELISA. Fluorescently-labeled bovine serum albumin infiltrating through lower chamber of Transwell was measured by multifunction microplate reader. Morphologic change of ciGENC was observed under inverted phase contrast microscope. The concentration of sflt-1 in preeclampsia groups was significantly increased as compared with control group （P〈0.01）. Permeability in preeclampsia groups was significantly increased as compared with control group （P〈0.01）. By contrast with severe preeclampsia group, the permeability of ciGENC monolayer in mild preeclampsia group was decreased significantly （P〈0.05）. Intervention of exogenous VEGF significantly decreased permeability of ciGENC in preeclampsia groups. It was concluded that sFlt-1 increased ciGENC permeability by damaging integrity of endothelial barrier function.

关键词： preeclampsia serum glomerular endothelial cells permeability sFlt-1 VEGF

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The Effects of glomerular endothelial Cell Autophagy on Diabetic Nephropathy

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临床医学工程 2019年第S1期26卷 3-3页

作者： Zhang Ao Fan Lijing Zhang Zeyu Zhu Danqi Hu Biying Xie Ting Liao Zhennan The Fifth Clinical Medical College Affiliated to Guangzhou University of Chinese Medicine GuangDong Second Traditional Chinese Medicine HospitalGuangzhou 510095China Department of Nephrology the First Affiliated Hospital of Ji'nan UniversityGuangzhou 510630China

Diabetic nephropathy is one of the most common causes of end-stage renal disease worldwide and is associated with increased mortality in patients with type 1 and type 2 *** is a highly conserved"autophagy"pathway and is an important mechanism for maintaining glomerular and tubular *** evidence suggests that targeted autophagy pathway activation and restoration of autophagy activity may have renal *** endothelial cells are an important key factor in the development of diabetic *** dysfunction is involved in the development of diabetic and non-diabetic glomerular injury and renal *** of glomerular endothelial dysfunction in the late stages of diabetic nephropathy,such as thrombotic microangiopathy,including impairment of glomerular capillary microaneurysms and glomerular membrane lysis autophagy,is related to the pathogenesis of diabetic ***,we mainly introduced the research progress of the effects of glomerular endothelial cell autophagy on diabetic nephropathy.

关键词： Diabetic nephropathy Autophagy glomerular endothelial cells

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