Carbon Monoxide Prevents Cyclosporine A-induced Injury of Glomerular Endothelial Cells

作     者：Zhe Zhou~(1,2),Yi Wu~(1,2),Meng Xiang~1,Dan Meng~1,Li Wang~1, Si-feng Chen~(1*),Wen-jun Yuan~(2*) (1.Departent of Physiology and Pathophysiology,Fudan University Shanghai Medical College, Shanghai,China 2.Departent of Physiology,Ningxia Medical University,Yinchuan,China) 

会议名称：《第八届海峡两岸心血管科学研讨会》

会议日期：2011年

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

关 键 词：cyclosporin A heme oxygenase-1 carbon monoxide CORM -2 glomerular endothelial cells endoplasmic reticulum stress 

摘      要：正Objective Most kidney allografts eventually lose their function because of chronic *** A（CsA） is one of the most commonly used immunosuppressive *** preventing allograft immune rejection,CsA may injure many renal *** oxygenase-1（HO-1） is protective for numerous cells including renal epithelial cells by presenting anti-inflammatory,anti-oxidative and anti-apoptotic properties through its catalyzed products such;as carbon monoxide（CO）.However,the effects of CsA on glomerular endothelial cells（GECs） and the role of Ho-1 arenot *** study observed the effects of HO -1 and CO on CsA-induced GEC injury and *** and Methods Primary GECs were treated with 25μmol/L CsA to induce injury and *** and CORM -2 were used for representatives for HO-1 inducers and HO-1 catalyzed products,*** expressions of HO-1 and endoplasmic reticulum stress response（ER） proteins（CHOP and ATF -6） were evaluated by Western bolting. Cell apoptosis,proliferation and migration were measured by annexin-V and PI double staining,MTT degradation assay and cell monolayer scratch assays,*** At 8 h after treatment,HO-1 and CHOP expression in CsA-treated cells increased significantly compared to untreated *** promoted apoptosis and inhibited cell proliferation and migration of the *** increased HO-1 protein and mRNA expressions *** hemin decreased CsA -induced CHOP expression,it did not prevent CsA -induced apoptosis,proliferation and migration inhibitions compared to untreated ***-releasing molecule CORM-2 did not affect biological properties and protein expressions of the cells without CsA treatment although it decreased migration of the ***,it blocked CsAinduced cell proliferation inhibition, apoptosis and CHOP expression although it did not change cell migration,ATF-6 expression and activation of CsA-treated *** Pharmacological dose of Cs