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Molecular Plant 2012年第4期5卷 852-864页

作者： Li-Hua Hao Wei-Xia Wang Chen Chen Yu-Fang wang Ting Liu Xia Li Zhong-Lin Shang a Key Laboratory of Molecular and Cell Biology of Hebei Province College of Life SciencesHebei Normal UniversityShijiazhuang 050016RR.China State Key Laboratory of Plant Cell and Chromosome Engineering Center of Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050021P.R.China

In recent years, adenosine tri-phosphate （atp） has been reported to exist in apoplasts of plant cells as a signal molecule. extracellular atp （eatp） plays important roles in plant growth, development, and stress tolerance. Here, extra- cellular atp was found to promote stomatal opening of Arabidopsis thaliana in light and darkness. ADP, GTP, and weakly hydrolyzable atp analogs （atpγS, Bz-atp, and 2meatp） showed similar effects, whereas AMP and adenosine did not affect stomatal movement. Apyrase inhibited stomatal opening. atp-promoted stomatal opening was blocked by an NADPH oxidase inhibitor （diphenylene iodonium） or deoxidizer （dithiothreitol）, and was impaired in null mutant of NADPH ox- idase （atrbohD/F）. Added atp triggered ROS generation in guard cells via NADPH oxidase. atp also induced Ca^2＋ influx and H ＋ efflux in guard cells. In atrbohD/F, atp-induced ion flux was strongly suppressed. In null mutants of the heterotrimeric G protein α subunit, atp-promoted stomatal opening, cytoplasmic ROS generation, Ca^2＋ influx, and ^H＋ efflux were all sup- pressed. These results indicated that eatp-promoted stomatal opening possibly involves the heterotrimeric G protein, ROS, cytosolic Ca^2＋, and plasma membrane H＋-atpase.

关键词： extracellular atp stomatal movement Arabidopsis thaliana heterotrimeric G protein reactive oxygenspecies.

Cell viability in the cadmium-stressed cell suspension cultures of tobacco is regulated by extracellular atp,possibly by a reactive oxygen species-associated mechanism

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BIOCELL 2020年第1期44卷 89-99页

作者： Zhenzhen SHI Jiaxin CAO Hailong PANG Lingyun JIA Kun SUN Hanqing FENG College of Life Science Northwest Normal UniversityLanzhou730070China

Cadmium(Cd)is one of the most widespread and toxic heavy metals to *** atp(exatp)is thought to be an extracellular effector in regulating the physiological responses of plant cells to environmental ***,the function of exatp in Cd-stressed plant cells is much *** present work showed that treating tobacco(Nicotiana tabacum *** Yellow-2)cell-suspension cultures with exogenous CdCl2 reduced the cell viability,exatp level,and Mg ***,the production of reactive oxygen species(ROS),Cd content,and electrolyte leakage of the cells were enhanced by exogenous *** the Cd-induced accumulation of ROS was decreased by the supplement with DMTU(dimethylthiourea,a scavenger of ROS),the Cd-induced increases of the electrolyte leakage and Cd content were alleviated,and the Cd-induced reductions of cell viability were partly rescued,suggesting that Cd-induced reduction of cell viability could be related to the ROS *** the condition of Cd stress,when the reduction of exatp level was partly rescued by exogenous atp(20μM),the increases of ROS production,electrolyte leakage,and Cd content were attenuated,and the reduction of cell viability was also *** observations indicate that exatp can regulate the cell viability in the Cd–stressed plant cells possibly by an ROS-associated mechanism.

关键词： Cell viability Cd ions extracellular atp Reactive oxygen species

Therapeutic potential of extracellular atp and P2 receptors in nervous system diseases

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Neuroscience Bulletin 2009年第1期25卷 27-32页

作者：屠洁王立平中国科学院深圳先进技术研究院神经工程研究中心深圳518067 香港大学医学院生理系

extracellular adenosine 5’-triphosphate(atp) is a key signaling molecule present in the central nervous system(CNS),and now is receiving greater attention due to its role as a messenger in the CNS during different physiological and pathological events. atp is released into the extracellular space through vesicular exocytosis or from damaged and dying cells. Once in the extracellular environment,atp binds to the specific receptors termed P2,which mediate atp effects and are present broadly in both neurons an...

关键词： extracellular atp P2 receptors nervous system diseases

Effects of extracellular atp and Its Receptors on Peripheral Nerve Regeneration in SD Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第1期23卷 35-37页

作者：王栓科张致英洪光祥王同光王发斌康皓 DepartmentofOrthopaedics SecondAffiliatedHospitalofLanzhouMedicalCollege DepartmentofHandSurgery XieheHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology

To explore the effect of the extracellular atp and its receptors on axon regeneration in the sciatic nerve defect in rats, 0.5 cm defect of the sciatic nerve was made and repaired with long arm silicon tube like a 'Y' type. The single arm of the silicon tube was sutured to proximal of the sciatic nerve. 10 μl 1 mmol/L atp in physiological saline was injected into the left chamber of the silicon tube (experimental group) and physiological saline injected into another silicon tube as a control group. In another model 1 mmol/L 10 μl atp and 1 mmol/L 10 μl atp+0.2 mg/ml suramin were injected respectively into two arms of the silicon tube. After 4 and 8 weeks the specimens were obtained from the silicon tube for examining the axon regeneration histologically and image analysis. All the regeneration axons grew into the silicon tube containing the atp, but there was no axon regeneration in the silicon tube containing the atp+Suramin or physiological saline. It was demonstrated that extracellular atp had a powerful attraction to the regenerated axon of peripheral nerve. The suramin inhibited the axon induction of the extracellular atp completely via the atp receptors.

关键词： extracellular atp nerve axon regeneration atp receptors Suramin

Effects of extracellular atp on Survival of Sensory Neurons in the Dorsal Root Ganglia of Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第1期21卷 44-47页

作者：王同光洪光祥王拴科王发斌黄启顺翁雨雄 Department of Hand Surgery Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology

atp was added to the cultured sensory neurons obtained from the dorsal root ganglia of the neonatal rats and PBS was added to serve as control. MTT assays were conducted to evaluate the survival and activity of the cultured neurons. And the silicone regenerative chamber was used after the sciatic nerve incision of the mature SD rat. 1 mmol/L atp was injected into the left chamber and 0.09 % natrium chloride was injected into the right chamber as controls. The changes of nitric oxide synthase (NOS) activity in the corresponding dorsal root ganglia were measured histochemically and image analysis was also performed 4 days after the sciatic nerve injury. The results showed that extracellular atp could enhance the survival of the neurons and the number of NOS positive neurons were significantly different between the atp and control groups ( P extracellular atp had neurotrophic effect on neurons survival and could inhibit the NOS activity of the sensory neurons after the peripheral nerve incision, hence exerting the protective effect on the neurons, which was valuable for nerve regeneration after nerve injury.

关键词： extracellular atp neurotrophins neurons nitric oxide synthase Sprague Dawley rats

Drug resistance and combating drug resistance in cancer

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Cancer Drug Resistance 2019年第2期2卷 141-160页

作者： Xuan Wang Haiyun Zhang Xiaozhuo Chen Department of Biological Sciences Ohio UniversityAthensOH 45701USA Interdisciplinary Graduate Program in Molecular and Cellular Biology Ohio UniversityAthensOH 45701USA The Edison Biotechnology Institute Ohio UniversityAthensOH 45701USA Department of Chemistry and Biochemistry Ohio UniversityAthensOH 45701USA Department of Biomedical Sciences Heritage College of OsteopathicOhio UniversityAthensOH 45701USA.

Cancer is the second leading cause of death in the *** major treatments for cancer management include surgery,cytotoxic chemotherapy,targeted therapy,radiation therapy,endocrine therapy and *** the endeavors and achievements made in treating cancers during the past decades,resistance to classical chemotherapeutic agents and/or novel targeted drugs continues to be a major problem in cancer *** resistance,either existing before treatment(intrinsic)or generated after therapy(acquired),is responsible for most relapses of cancer,one of the major causes of death of the *** among patients and tumors,and the versatility of cancer to circumvent therapies make drug resistance more challenging to deal *** understanding the mechanisms of drug resistance is required to provide guidance to future cancer treatment and achieve better *** this review,intrinsic and acquired resistance will be *** addition,new discoveries in mechanisms of drug resistance will be ***,we will highlight roles of atp in drug resistance by discussing recent findings of exceptionally high levels of intratumoral extracellular atp as well as intracellular atp internalized from extracellular *** complexity of drug resistance development suggests that combinational and personalized therapies,which should take atp into consideration,might provide better strategies and improved efficacy for fighting drug resistance in cancer.

关键词： Cancer stem cells epithelial mesenchymal transition atp binding cassette transporters extracellular atp macropinocytosis epigenetics microRNA

P2X7 Receptor Mediated Growth-Inhibitory Effect in KG1a Cell Line

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Chinese Journal of Clinical Oncology 2008年第6期5卷 400-406页

作者： Xiujun Zhang Lijun Meng Department of Biological Sciences North China Coal Medical College Tangshan 063000 Heibei Province China Department of Environment ＆ Chemical Engineering Tangshan College Tangshan 063000. Heibei Province China

OBJECTIVE This study was conducted to investigate atp- induced growth inhibition in human leukemic cells KG1a. METHODS atp inhibited cell growth was analyzed by MTS assay. Externalization of phosphatidylserine could be detected by Annexin-V-FITC apoptosis staining after activation of the P2X7 receptor. P2X7 mediated pore formation was detected in KGla cells by Yo-Pro-1 uptake assay. RESULTS atp inhibited cell growth in a dose-dependent manner. The cytotoxic effect could be blocked by P2X7 antagonists, oxidized atp （oatp） and KN62. Externalization of phosphatidylserine could be detected in a time-dependent manner. P2X7 mediated pore formation could be detected in KG1a cells. These effects could not be observed in P2X7 null Ramos cells. CONCLUSION The results and our previously reports that mRNA, protein expression and calcium response of the P2X7 receptor in KGla cells, suggested that extracellular atp effectively induces growth inhibition through apoptosis in KGla cells by activation of P2X7 receptor, and that may be mediated by extracellular Ca^2＋ in ux and pore formation.

关键词： P2X7 receptor growth inhibition apoptosis extracellular atp leukemia cells.