Effects of Extracellular ATP and Its Receptors on Peripheral Nerve Regeneration in SD Rats

作     者：王栓科 张致英 洪光祥 王同光 王发斌 康皓 WANG Shuanke , ZHANG Zhiying Department of Orthopaedics, Second Affiliated Hospital of Lanzhou Medical College, Lanzhou 730030HONG Guangxiang , WANG Tongguang , WANG Fabin , KANG Hao Department of Hand Surgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022

作者机构：DepartmentofOrthopaedicsSecondAffiliatedHospitalofLanzhouMedicalCollege DepartmentofHandSurgeryXieheHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2003年第23卷第1期

页      面：35-37页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：extracellular ATP nerve axon regeneration ATP receptors Suramin 

摘      要：To explore the effect of the extracellular ATP and its receptors on axon regeneration in the sciatic nerve defect in rats, 0.5 cm defect of the sciatic nerve was made and repaired with long arm silicon tube like a Y type. The single arm of the silicon tube was sutured to proximal of the sciatic nerve. 10 μl 1 mmol/L ATP in physiological saline was injected into the left chamber of the silicon tube (experimental group) and physiological saline injected into another silicon tube as a control group. In another model 1 mmol/L 10 μl ATP and 1 mmol/L 10 μl ATP+0.2 mg/ml suramin were injected respectively into two arms of the silicon tube. After 4 and 8 weeks the specimens were obtained from the silicon tube for examining the axon regeneration histologically and image analysis. All the regeneration axons grew into the silicon tube containing the ATP, but there was no axon regeneration in the silicon tube containing the ATP+Suramin or physiological saline. It was demonstrated that extracellular ATP had a powerful attraction to the regenerated axon of peripheral nerve. The suramin inhibited the axon induction of the extracellular ATP completely via the ATP receptors.