检索结果-南通市图书馆

Morphofunctional characterization of hemocytes in black soldier fly larvae

Insect Science 2023年第4期30卷 912-932页

作者： Daniele Bruno Aurora Montali Marzia Gariboldi Anna Katarzyna Wronska Agata Kaczmarek Amr Mohamed Ling Tian Morena Casarteli Gianluca Tettamanti Department of Biotechnology and Life Sciences University of InsubriaVareseItaly Host Parasites Molecular Interaction Research Unit Witold Stefanski Institute of ParasitologyPolish Academy of SciencesWarsawPoland Department of Entomology Faculty of ScienceCairo UniversityGizaEgypt Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding Guangdong Provincial Sericulture and Mulberry Engineering Research CenterCollege of Animal ScienceSouth China Agricultural UniversityGuangzhouChina Department of Biosciences University of MilanoMilanoItaly Interuniversity Centerfor Studies on Bioinspired Agro-environmental Technology(BAT Center) University of Napoli Federico llPorticiItaly

In insects,the cell-mediated immune response involves an active role of hemo-cytes in phagocytosis,nodulation,and encapsulation.Although these processes have been well documented in multiple species belonging to different insect orders,information con-cerning the immune response,particularly the hemocyte types and their specific function in the black soldier fly Hermetia illucens,is still limited.This is a serious gap in knowledge given the high economic relevance of H.illucens larvae in waste management strategies and considering that the saprophagous feeding habits of this dipteran species have likely shaped its immune system to efficiently respond to infections.The present study repre-sents the first detailed characterization of black soldier fly hemocytes and provides new insights into the cell-mediated immune response of this insect.In particular,in addition to prohemocytes,we identified five hemocyte types that mount the immune response in the larva,and analyzed their behavior,role,and morphofunctional changes in response to bac-terial infection and injection of chromatographic beads.Our results demonstrate that the circulating phagocytes in black soldier fly larvae are plasmatocytes.These cells also take part in nodulation and encapsulation with granulocytes and lamellocyte-like cells,devel-oping a starting core for nodule/capsule formation to remove/encapsulate large bacterial aggregates/pathogens from the hemolymph,respectively.These processes are supported by the release of melanin precursors from crystal cells and likely by mobilizing nutrient reserves in newly circulating adipohemocytes,which could thus trophically support other hemocytes during the immune response.Finally,the regulation of the cell-mediated im-mune response by eicosanoids was investigated.

关键词： eicosanoids encapsulation hemocytes Hermetia illucens immunity phagocytosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

eicosanoids mediate nodulation reactions to bacterial Escherichia coil K 12 infections in larvae of the oriental blowfly, Chrysomya megacephala

引用

Insect Science 2009年第5期16卷 387-392页

作者： Fu Zhao Bing Chen Yong Wang Fen Zhu Chao-Liang Lei Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory Huazhong Agricultual University Wuhan China State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences BeijingChina

Nodulation is the predominant cellular defense reaction to bacterial challenges in insects. In this study, third instar larvae of Chrysomya megacephala were injected with bacteria, Escherichia coli K 12 （10^6 CFU/mL, 2μL）, immediately prior to injection of inhibitors of eicosanoid biosynthesis, which sharply reduced nodulation response. Test larvae were treated with specific inhibitors ofphospholipase A2 （dexamethasone）, cyclo- oxygenase （indomethacin, ibuprofen and piroxicam）, dual cyclo-oxygenase/lipoxygenase （phenidone） and lipoxygenase （esculetin） and these reduced nodulation except esculetin. The influence of bacteria was obvious within 2 h of injection （5 nodules/larva）, and increased to a maximum after 8 h （with 15 nodules/larva）, and then significantly reduced over 24 h （9 nodules/larva）. The inhibitory influence of dexamethasone was apparent within 2 h of injection （4 vs. 5 nodules/larva）, and nodulation was significantly reduced, compared to control, over 24 h （5 vs. 8 nodules/larva）. Increased dosages of ibuprofen, indomethacin, piroxicam and phenidone led to decreased numbers of nodules. Nodules continued to exist during the pupal stage. However, the effects of dexamethasone were reversed by treating bacteria-injected insects with an eicosanoid-precursor polyunsaturated fatty acid, arachidonic acid. These findings approved our view that eicosanoid can mediate cellular defense mechanisms in response to bacterial infections in another Dipteran insect C. rnegacephala.

关键词： bacteria Chrysomya megacephala eicosanoids Escherichia coli K12 nodulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Deciphering lipid dysregulation in ALS:from mechanisms to translational medicine

引用

Translational Neurodegeneration 2022年第1期11卷 192-218页

作者： Ira Agrawal Yong Shan Lim Shi-Yan Ng Shuo-Chien Ling Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeTahir Foundation BuildingMD116-03-H12 Science Drive 2Singapore 117549Singapore Healthy Longevity Translational Research Programme Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore Institute of Molecular and Cell Biology A*STAR Research EntitiesSingaporeSingapore Program in Neuroscience and Behavior Disorders Duke-National University of Singapore Medical SchoolSingaporeSingapore.

Lipids,defined by low solubility in water and high solubility in nonpolar solvents,can be classified into fatty acids,glycerolipids,glycerophospholipids,sphingolipids,and sterols.Lipids not only regulate integrity and fluidity of biologi-cal membranes,but also serve as energy storage and bioactive molecules for signaling.Causal mutations in SPTLC1(serine palmitoyltransferase long chain subunit 1)gene within the lipogenic pathway have been identified in amyo-trophic lateral sclerosis(ALS),a paralytic and fatal motor neuron disease.Furthermore,lipid dysmetabolism within the central nervous system and circulation is associated with ALS.Here,we aim to delineate the diverse roles of different lipid classes and understand how lipid dysmetabolism may contribute to ALS pathogenesis.Among the different lipids,accumulation of ceramides,arachidonic acid,and lysophosphatidylcholine is commonly emerging as detri-mental to motor neurons.We end with exploring the potential ALS therapeutics by reducing these toxic lipids.

关键词： Amyotrophic lateral sclerosis Sphingolipids Triglycerides Phospholipids Cholesterol esters Fatty acids Ceramides Arachidonic acid Lysophosphatidylcholine eicosanoids

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Increased Soluble Epoxide Hydrolase Activity Positively Correlates with Mortality in Heart Failure Patients with Preserved Ejection Fraction:Evidence from Metabolomics

引用

Phenomics 2023年第1期3卷 34-49页

作者： Liyuan Peng Ziping Song Chengcheng Zhao Kudusi Abuduwufuer Yanwen Wang Zheng Wen Li Ni Chenze Li Ying Yu Yi Zhu Hualiang Jiang Jinshan Shen Xiangrui Jiang Chen Chen Xu Zhang Dao Wen Wang Division of Cardiology Department of Internal MedicineTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders Wuhan 430030China Department of Pharmacology Tianjin Key Laboratory of Inflammation BiologyKey Laboratory of Immune Microenvironment and Disease(Ministry of Education)School of Basic Medical SciencesTianjin Medical UniversityTianjin 300070China Tianjin Key Laboratory of Metabolic Diseases Collaborative Innovation Center of Tianjin for Medical EpigeneticsCenter for Cardiovascular DiseasesDepartment of Physiology and PathophysiologyResearch Center of Basic Medical SciencesTianjin Medical UniversityTianjin 300070China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 200032China CAS Key Laboratory for Receptor Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 200032China Tianjin Key Laboratory of Metabolic Diseases Key Laboratory of Immune Microenvironment and Disease-Ministry of EducationDepartment of Physiology and PathophysiologyCollaborative Innovation Center of Tianjin for Medical EpigeneticsTianjin Medical UniversityTianjin 300070China

Epoxyeicosatrienoic acids(EETs)have pleiotropic endogenous cardiovascular protective effects and can be hydrolyzed to the corresponding dihydroxyeicosatrienoic acids by soluble epoxide hydrolase(sEH).Heart failure with preserved ejection fraction(HFpEF)has shown an increased prevalence and worse prognosis over the decades.However,the role of sEH activ-ity in HFpEF remains unclear.We enrolled 500 patients with HFpEF and 500 healthy controls between February 2010 and March 2016.Eight types of sEH-related eicosanoids were measured according to target metabolomics,and their correlation with clinical endpoints was also analyzed.The primary endpoint was cardiac mortality,and the secondary endpoint was a composite of cardiac events,including heart failure(HF)readmission,cardiogenic hospitalization,and all-cause mortal-ity.Furthermore,the effect of sEH inhibitors on cardiac diastolic function in HFpEF was investigated in vivo and in vitro.Patients with HFpEF showed significantly enhanced EET degradation by the sEH enzyme compared with healthy controls.More importantly,sEH activity was positively correlated with cardiac mortality in patients with HFpEF,especially in older patients with arrhythmia.A consistent result was obtained in the multiple adjusted models.Decreased sEH activity by the sEH inhibitor showed a significant effective effect on the improvement of cardiac diastolic function by ameliorating lipid disorders in cardiomyocytes of HFpEF mouse model.This study demonstrated that increased sEH activity was associated with cardiac mortality in patients with HFpEF and suggested that sEH inhibition could be a promising therapeutic strategy to improve diastolic cardiac function.Clinical trial identifier:NCT03461107(https://clini caltr ials.gov).

关键词： Soluble epoxide hydrolase Heart failure with preserved ejection fraction eicosanoids Epoxyeicosatrienoic acids

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of emodin and sandostatin on metabolism of eicosanoids in acute necrotizing pancreatitis

引用

World Journal of Gastroenterology 2000年第2期6卷 293-294页

作者： Jian Xin Wu Jia Yu Xu Yao Zong Yuan Department of Gastroenterology Shanghai First People’s HospitalShanghai 200080China Ruijin Hospital Shanghai Second Medical UniversityShanghai 200025China

INTRODUCTIONIn order to study the therapeutic mechanisms ofemodin,an extract of Rhubarb （Rhizoma et RadixRhei,a traditional Chinese herbal medicine）,andsandostatin in the treatment of acute necrotizingpancreatitis （ANP）,we used the two drugs in ratmodels of the disease and observed the changes ofplasma thromboxane-2（TXB2）,6-keto-prostaglandin F1α(6-keto-PGF1α)and prostaglandinE2（PEG2）.

关键词： pancreatitis eicosanoids metabolism emodin sandostatin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A target lipidomics approach to investigate the acute inflammatory irritation induced by indolealkylamines from Chansu water fraction in rats

引用

Chinese Journal of Natural Medicines 2021年第11期19卷 856-867页

作者： YANG Xin CHEN Wu-Yue GONG Yan DI Liu-Qing DUAN Jin-Ao ZHOU Jing MA Hong-Yue Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology Research of TCM FormulaeCollege of PharmacyNanjing University of Chinese MedicineNanjing 210023China Jiangsu Engineering Research Center for Efficient Delivery System of TCM Nanjing University of Chinese MedicineNanjing 210023China

Chansu has demonstrated adverse reactions in clinical settings,which is associated with its toxicity and limits its clinical applications.But there are methodological limitations for drug safety evaluation.In the current study,ultra-high performance liquid chromatography,lipidomic profiling,and molecular docking were used to systemically assess Chansu-induced acute inflammatory irritation and further identify the underlying drug targets.Compared with the EtOAc extract,Chansu water fraction containing indolealkylamines caused acute inflammatory irritation in rats,including acute pain(spontaneous raising foot reaction),and inflammation(paw edema).At the molecular level,lipids analysis revealed significantly higher levels of pro-inflammatory mediators of the COX and LOX pathways.However,anti-inflammatory mediators from the CYP 450,ALA,and DHA pathways markedly decreased after exposure to Chansu water fraction.Moreover,four indolealkylamines from Chansu showed a high theoretical affinity to a known irritation target,5-HT_(2A)R.These results suggest that Chansu-induced inflammatory irritation is related to the distinct dysregulation of inflammatory lipids,and peripheral 5-HT_(2A)R is a potential target for irritation therapy.The strategy used in this study can be a crucial approach in the safety evaluation of natural medicinal substances.

关键词： Chansu Acute inflammatory irritation Lipidomics eicosanoids Molecular docking

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hyperhomocysteinemia dysregulates plasma levels of polyunsaturated fatty acids-derived eicosanoids

引用

Life Research 2022年第2期5卷 32-41页

作者： Mohamed Al-Shabrawey Ahmed Elmarakby Yara Samra Mohamed Moustafa Stephen WLooney Krishna Rao Maddipati Amany Tawfik Department of Foundational Medical Studies and Eye Research Center Oakland University William Beaumont School of MedicineRochesterMichiganUSA Eye Research Institute Oakland UniversityRochesterMichiganUSA Department of Oral Biology and Diagnostic Sciences Dental College of GeorgiaAugusta UniversityAugustaGeorgiaUSA Departments of Pharmacology&Toxicology Faculty of PharmacyMansoura UniversityMansouraEgypt Department of Biochemistry Faculty of PharmacyMansoura UniversityEgypt Department of Population Health Sciences Medical College of GeorgiaAugusta UniversityAugustaGeorgiaUSA Bioactive Lipids Research Program Department of PathologyWayne State UniversityMichiganUSA

Hyperhomocysteinemia(HHcy)contributes to the incidence of many cardiovascular diseases(CVD).Our group have previously established crucial roles of eicosanoids and homocysteine in the incidence of vascular injury in diabetic retinopathy and renal injury.Using cystathionine-β-synthase heterozygous mice(cβs^(+/-))as a model of HHcy,the current study was designed to determine the impact of homocysteine on circulating levels of lipid mediators derived from polyunsaturated fatty acids(PUFA).Plasma samples were isolated from wild-type(WT)and cβs^(+/-)mice for the assessment of eicosanoids levels using LC/MS.Plasma 12/15-lipoxygenase(12/15-LOX)activity significantly decreased in cβs^(+/-)vs.WT control mice.LOX-derived metabolites from both omega-3 and omega-6 PUFA were also reduced in cβs^(+/-)mice compared to WT control(Peicosanoids metabolism through decreasing LOX and COX metabolic activities while increasing CYP metabolic activity.The increase in AA metabolism by CYP was also associated with increase in sEH activity and decrease in EETs bioavailability.Dysregulation of eicosanoids metabolism could be a contributing factor to the incidence and progression of HHcy-induced CVD.

关键词： homocysteine cystathionine β-synthase eicosanoids cycloxygenase lipoxygenase cytochrome-P450

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hypertrophic Scar Formation and Wound Healing Modulation Fatty Acids as Modulators of Severe Scars

引用

Modern Plastic Surgery 2023年第1期13卷 41-51页

作者： Bárbara Díaz Valerie Nuñ ez Department of General Surgery Burn Unit Care Dr. Ricardo Gutiérrez Children’s Hospital Buenos Aires Argentina

Scar tissue usually generates severe discomfort in the short and long term. Common symptoms include anesthetics sequelae, pruritus, joint malfunction, new wounds on the scar surface, and pain. There are several treatments for scars, like compression, topical or intralesional steroid infiltration, 5-fluorouracil, dermabrasion, and surgeries with new scar tissue. For adult patients, it is easier to choose the treatment. However, compression is commonly applied in children to prevent treatments that have adverse effects. This study reports the outcomes of 15 patients submitted to abdominoplasty, traumatic wounds and post-burn scar treatments, which showed significant changes after the continuous use of an ointment composed of petrolatum, cod liver oil, BHT, Chamomilla recutita (chamomile) oil, Helianthus annuus (sunflower) oil, and Prunus amygdalus dulcis (sweet almond) oil. As components of the stratum corneum, unsaturated fatty acids influence the cutaneous structural and immune status and permeability. They also interfere with the maturation and differentiation of the stratum corneum and inhibit the production of proinflammatory eicosanoids, reactive species (ROS and RNS), and cytokines, thereby influencing the inflammatory response and possibly wound healing. This article aims to share our experience with the regular use of an ointment in adult and pediatric patients for three months. The increase in proinflammatory cytokine production at wound sites, resulting in a noninvasive, therapeutical, and effective cutaneous wound healing and scarring modulation, may provide a physiopathological explanation for the fast improvement of scars.

关键词： Scarring Burn Scar Inflammatory Modulation Cytokine Sequelae Fatty Acids eicosanoids Non-Adverse Effects Aesthetics Hypertrophic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cytochrome P450 monooxygenase-mediated eicosanoid pathway:A potential mechanistic linkage between dietary fatty acid consumption and colon cancer risk

引用

Food Science and Human Wellness 2019年第4期8卷 337-343页

作者： Weicang Wang Jianan Zhang Guodong Zhang Department of Food Science University of Massachusetts-AmherstAmherstMA01003USA Molecular and Cellular Biology Program University of Massachusetts-AmherstAmherstMA01003USA

Human consumption of linoleic acid(LA,18:2ω-6,abundant in vegetable oils)is very high.Animal experiments showed that excessive LA intake increased azoxymethane-induced colon tumorigenesis,however,the impact of excessive LA on colon cancer in human is not conclusive,making it difficult to make dietary recommendations for optimal intake of LA.Understanding the molecular mechanisms of LA on colon tumorigenesis could help to clarify its health effect,and facilitate development of mechanismbased strategies for preventing colon cancer.Recent studies show that the previously unappreciated cytochrome P450 monooxygenase-mediated eicosanoid pathway is upregulated in colon cancer and plays critical roles in its pathogenesis,and could contribute to the effects of dietary LA,as well asω-3 fatty acids,on colon tumorigenesis.In this review,we will discuss recent studies about the roles of cytochrome P450 monooxygenases in fatty acid metabolism and its roles in colonic inflammation and colon cancer,and how this information could help us to clarify the health impacts of dietary fatty acids.

关键词： Linoleic acid Polyunsaturated fatty acids ω-3 Fatty acids Colon cancer Colonic inflammation Cytochrome P450 eicosanoids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The 2-Series eicosanoids in Cancer: Future Targets for Glioma Therapy?

引用

Journal of Cancer Therapy 2013年第1期4卷 338-352页

作者： Tiberiu Moga Sunit Das Division of Neurosurgery St. Michael’s Hospital Toronto Canada

The 2-series eicosanoids are structurally related lipid-soluble hormones synthesized by cyclooxygenase enzymes from arachidonic acid. These compounds have well-established roles in the inflammatory response and the coagulation cascade. More recently, the eicosanoids have garnered attention for their potential roles in cancers of the lung, colon, breast, and brain. In this paper, we review the contributions of the different cyclooxygenase metabolites (i.e. prostaglandins, prostacyclins and thromboxanes) to cancer development, progression and recurrence, with special attention paid to their relevance to glioma biology. Our review suggests that 2-series eicosanoids merit further study as possible targets for therapy in patients with glioma.

关键词： eicosanoids Prostaglandins Prostacyclins Thromboxanes Cancer Glioma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：