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ectopic expression of the Pttknl Gene Induces Alterations in the Morphology of the Leaves and Flowers in Petunia hybrida Vilm.

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Journal of Integrative Plant Biology 2005年第10期47卷 1153-1158页

作者： Xin HU Qing-Feng WU Ya-Hong XIE Hong RU Feng XIE Xin-Yu WANG Chong-Ying WAN School of Life Sciences Lanzhou University Lanzhou 730000 China Xi＇an Institute of Gardens Xi＇an 710065 China

A novel knottedl-like homeobox （knox） gene, Pttknl （Populus tremulaxtremuloides knottedl）, isolated from the cambial region of hybrid aspen, was introduced into Petunia hybrida Vilm. using the leafdisc method mediated by Agrobacterium. A series of novel phenotypes was observed in transgenic petunia plants, including the formation of ectopic spikes on the adaxial surface of corollas and small petals on the abaxial surface of corollas, fusion of floral organs, shortening of corolla midribs, the formation of tumor-like knots along the midrib on the abaxial surface and serrated lobs of corolla margins, and alterations in petal color; except for changes in the leaves and plant architecture, RT-PCR showed that the Pttknl gene was expressed in the leaves of different petunia transgenic plants, whereas no signal was detected in wild-type plants. The possible function of Pttknl in leaf and flower development is discussed.

关键词： ectopic expression knox gene novel phenotypes Petunia hybrida Pttknl gene RT-PCR.

ectopic expression of antifreeze protein gene from Ammopiptanthus nanus confers chilling tolerance in maize

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The Crop Journal 2021年第4期9卷 924-933页

作者： Yuanyuan Zhang Yang Cao Hongying Zheng Wenqi Feng Jingtao Qu Fengling Fu Wanchen Li Haoqiang Yu Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of AgricultureMaize Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina College of Life Science&Biotechnology Mianyang Teachers’CollegeMianyang 621000SichuanChina

Improved chilling tolerance is important for maize production. Previous efforts in transgenics and marker-assisted breeding have not achieved practical results. In this study, the antifreeze protein(AnAFP) from the super-xerophyte Ammopiptanthus nanus was aligned to KnS-type *** in vitro and subcellular localization showed that AnAFP was phosphorylated by maize casein kinase II and translocated from nucleus to cytoplasm under chilling stress. AnAFP also increased lactate dehydrogenase activity. A parent line of commercial maize hybrids was transformed with the AnAFP gene. Based on thermal asymmetric interlaced PCR, one hemizygous and two homozygous integration sites were identified in one T_(1) line. ectopic expression of AnAFP in transgenic lines was confirmed by quantitative real-time PCR, RNA-seq, and Western blotting. After chilling treatment, the transgenic lines showed significantly improved phenotype, higher kernel production, survival rate and biomass, and lower relative electrolyte leakage and superoxide dismutation than the untransformed line. Thus, ectopic expression of AnAFP gene improved chilling tolerance in the transgenic lines, which could be used to apply for further safety assessment for commercial breeding.

关键词： Antifreeze protein Chilling tolerance Dehydrin ectopic expression Homozygosity identification

Olfactory receptors in neural regeneration in the central nervous system

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Neural Regeneration Research 2025年第9期20卷 2480-2494页

作者： Rafael Franco Claudia Garrigós Toni Capó Joan Serrano-Marín Rafael Rivas-Santisteban Jaume Lillo Molecular Neurobiology Laboratory Department of Biochemistry and Molecular BiomedicineFacultat de BiologiaUniversitat de BarcelonaBarcelonaSpain CiberNed Network Center for Biomedical Research in Neurodegenerative Diseases Spanish National Health Institute Carlos ⅢMadridSpain School of Chemistry Universitat de BarcelonaBarcelonaSpain Department of Biochemistry and Physiology School of Pharmacy and Food SciencesUniversitat de BarcelonaBarcelonaSpain Laboratory of Computational Medicine Biostatistics UnitFaculty of MedicineAutonomous University of BarcelonaCampus BellaterraBarcelonaSpain

Olfactory receptors are crucial for detecting odors and play a vital role in our sense of smell,influencing behaviors from food choices to emotional *** receptors also contribute to our perception of flavor and have potential applications in medical diagnostics and environmental *** ability of the olfactory system to regenerate its sensory neurons provides a unique model to study neural regeneration,a phenomenon largely absent in the central nervous *** gained from how olfactory neurons continuously replace themselves and reestablish functional connections can provide strategies to promote similar regenerative processes in the central nervous system,where damage often results in permanent *** the molecular and cellular mechanisms underpinning olfactory neuron regeneration could pave the way for developing therapeutic approaches to treat spinal co rd injuries and neurodegenerative diseases like Alzheimer's *** ctory receptors are found in almost any cell of eve ry orga n/tissue of the mammalian *** ectopic expression provides insights into the chemical structures that can activate olfactory *** addition to odors,olfactory receptors in ectopic expression may respond to endogenous compounds and molecules produced by mucosal colonizing *** analysis of the function of olfactory receptors in ectopic expression provides valuable information on the signaling pathway engaged upon receptor activation and the receptor's role in proliferation and cell differentiation *** review explo res the ectopic expression of olfa ctory receptors and the role they may play in neural regeneration within the central nervous system,with particular attention to compounds that can activate these receptors to initiate regenerative *** suggests that olfactory receptors could serve as potential therapeutic targets for enhancing neural repair and recovery following central nervous syste

关键词： adenosine receptors adrenergic receptors ectopic expression G proteincoupled receptors glia neurons

Regulatory network and genetic interactions established by OsMADS34 in rice inflorescence and spikelet morphogenesis

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Journal of Integrative Plant Biology 2017年第9期59卷 693-707页

作者： Qingcai Meng Xiaofeng Li Wanwan Zhu Li Yang Wanqi Liang Ludovico Dreni Dabing Zhang Joint International Research Laboratory of Metabolic&Developmental Sciences State Key Laboratory of Hybrid RiceShanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and HealthSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Instituto de Biología Molecular y Celular de Plantas Consejo Superior de Investigaciones Científicas-Universidad Politécnica de ValenciaValencia 46022Spain School of Agriculture Food and WineUniversity of AdelaideWaite CampusUrrbraeSA 5064Australia

Grasses display highly diversified inflorescence architectures that differ in the arrangement of spikelets and flowers and determine cereal yields. However, the molecular basis underlying grass inflorescence morphogenesis remains largely unknown. Here we investigate the role of a functionally diversified SEPALLATA MADS-box transcription factor, OsMADS34, in regulating rice （Oryza sativa L.） inflorescence and spikelet development. Microarray analysis showed that, at the very early stages of inflorescence formation, dysfunction of OsMADS34 caused altered expression of 379 genes that are associated with protein modification and degradation, transcriptional regulation, signaling and metabolism activity. Genetic analysis revealed that OsMADS34 controls different aspects of inflorescence structure, branching and meristem activity synergistically with LAX PANICLE1 （LAX1） and FLORAL ORGAN NUMBER4 （FON4）, as evidenced by the enhanced phenotypes of osmads34 lax1 and osmads34 fon4 compared with the single mutants. Additionally, double mutant between osmads34 and the sterile lemma defective mutant elongated empty glume （ele） displayed an enhanced phenotype, that is, longer and wider sterile lemmas that were converted into lemma/palea-like organs, suggesting that ELE and OsMADS34 synergistically control the sterile lemma development. OsMADS34 may act together with OsMADS15 in controlling sterile lemma development. Collectively, these findings provide insights into the regulatory function of OsMADS34 in rice inflorescence and spikelet development.

关键词： FLORAL ORGAN IDENTITY MADS-BOX GENES SHOOT APICAL MERISTEM STEM-CELL FATE FLOWER DEVELOPMENT HOMEOTIC GENES ORYZA-SATIVA AXILLARY MERISTEMS ectopic expression RECEPTOR KINASE

Intron-retained alternatively spliced VIN3-LIKE 2 gene from Chimonanthus praecox promotes flowering in transgenic Arabidopsis

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Horticultural Plant Journal 2023年第4期9卷 789-800页

作者： Tian Xu Ning Liu Chunyu Wu Yi Zhang Mingkang Tian Shunzhao Sui Zhineng Li Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Chongqing Engineering Research Center for FloricultureCollege of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China

Vernalization is a process of acquiring or accelerating the flowering ability by prolonged cold *** INSENSITIVE3(VIN3)is induced by chilling and is extremely important for the vernalization response in Arabidopsis ***,the issue of the induction of the VIN3-LIKE genes in wintersweet(Chimonanthus praecox)has been largely *** the present study,we explored the molecular regulation of the PHD type finger protein-encoding gene CpVIL2 in relation to the growth and development of wintersweet in *** wintersweet,quantitative real-time PCR(qRT-PCR)analysis showed that the relative expression of CpVIL2-As2i(intron-retained alternatively spliced in the second intron)was extremely higher in the pistils than in the other *** the relative CpVIL2-As2i expression in flower buds(FBs)treated at 8°C was higher than that of FBs in December,2016 under natural conditions,which was not detected in non-flowering FBs at 16°*** Arabidopsis,the expression patterns of the CpVIL2-As2i gene were detected at first in CpVIL2-As2i pro::GUS(β-glucuronidase)lines,with predominantly higher expression in flowers and ***,the hormone-induced expression profiles of the CpVIL2-As2i promoter were confirmed using exogenous induction by abscisic acid(ABA)and indole acetic acid(IAA)phytohormones,where the GUS enzyme activity obviously decreased compared with that of *** comparison with Arabidopsis/Col-0,early flowering was detected in ectopic 35S::CpVIL2-As2i ***,these results demonstrated the function of the CpVIL2-As2i gene,at the same time,provided us with new insights into the molecular mechanisms of early flowering and complex regulatory networks of vernalization in wintersweet.

关键词： Chimonanthus praecox PHD finger protein VIL2 ectopic expression Flowering time Vernalization

A Major QTL, Ghd8, Plays Pleiotropic Roles in Regulating Grain Productivity, Plant Height, and Heading Date in Rice

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Molecular Plant 2011年第2期4卷 319-330页

作者： Wen-Hao Yan Peng Wang Hua-Xia Chen Hong-Ju Zhou Qiu-Ping Li Chong-Rong Wang Ze-Hong Ding Yu-Shan Zhang Si-Bin Yu Yong-Zhong Xing Qi-Fa Zhang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan430070 China

Rice yield and heading date are two distinct traits controlled by quantitative trait loci （QTLs）. The dissection of molecular mechanisms underlying rice yield traits is important for developing high-yielding rice varieties. Here, we report the cloning and characterization of Ghd8, a major QTL with pleiotropic effects on grain yield, heading date, and plant height. Two sets of near isogenic line populations were developed for the cloning of GhdS. Ghd8 was narrowed down to a 20-kb region containing two putative genes, of which one encodes the OsHAP3 subunit of a CCAAT-box binding protein （HAP complex）; this gene was regarded as the Ghd8 candidate. A complementary test confirmed the identity and pleiotropic effects of the gene; interestingly, the genetic effect of Ghd8 was dependent on its genetic background. By regulating Ehdl, RFT1, and Hd3a, Ghd8 delayed flowering under long-day conditions, but promoted flowering under short-day conditions. Ghd8 up-regulated MOC1, a key gene controlling tillering and branching; this increased the number of tillers, primary and secondary branches, thus producing 50% more grains per plant. The ectopic expression of Ghd8 in Arabidopsis caused early flowering by 10 d-a situation similar to the one observed by its homolog AtHAP3b, when compared to wild-type under long-day conditions; these findings indicate the conserved function of Ghd8 and AtHAP3b in flowering in Arabidopsis. Our results demonstrated the important roles of Ghd8 in rice yield formation and flowering, as well as its opposite functions in flowering between rice and Arabidopsis under long-day conditions.

关键词： Near isogenic lines CCAAT-box binding protein ectopic expression flowering pathway yield formation

Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of“Candidatus Liberibacter asiaticus”in citrus

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Molecular Horticulture 2023年第1期3卷 197-213页

作者： Shuai Wang Meixia Du Liting Dong Rongrong Qu Danlu Ran Juanjuan Ma Xuefeng Wang Lanzhen Xu Weimin Li Yongrui He Xiuping Zou Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Citrus Research InstituteSouthwest University/National Citrus Engineering Research CenterChongqingPeople’s Republic of China Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural AffairsBeijing University of AgricultureBeijingPeople’s Republic of China

Citrus Huanglongbing(HLB),caused by Candidatus Liberibacter asiaticus(CaLas),is the most serious disease ***460 was previously characterized as a potential virulence factor of ***,the function and mechanism of CaLasSDE460 involved in CaLas against citrus is still ***,we showed that transgenic expression of CaLasSDE460 in Wanjincheng oranges(*** Osbeck)contributed to the early growth of CaLas and the development of *** the temperature increased from 25℃to 32℃,CaLas growth and symptom development in transgenic plants were slower than those in WT ***-seq analysis of transgenic plants showed that CaLasSDE460 affected multiple biological *** 25℃,transcription activities of the“Protein processing in endoplasmic reticulum”and“Cyanoamino acid metabolism”pathways increased while transcription activities of many pathways decreased at 32℃.124 and 53 genes,separately annotated to plant-pathogen interaction and MAPK signaling pathways,showed decreased expression at 32℃,compared with these(38 for plant-pathogen interaction and 17 for MAPK signaling)at 25℃.Several important genes(MAPKKK14,HSP70b,NCED3 and WRKY33),remarkably affected by CaLasSDE460,were ***,our data suggested that CaLasSDE460 participated in the pathogenesis of CaLas through interfering transcription activities of citrus defense response and this interfering was temperature-dependent.

关键词： Citrus HLB CaLasSDE460 Transcriptome ectopic expression

A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants

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Plant Communications 2023年第2期4卷 65-76页

作者： Pu Yan Decai Tuo Wentao Shen Haida Deng Peng Zhou Xinzheng Gao Key Laboratory of Biology and Genetic Resources of Tropical Crops Sanya Research InstituteInstitute of Tropical Biosciences and BiotechnologyChinese Academy of Tropical Agricultural Science&Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan ProvinceHainan Institute of Tropical Agricultural ResourcesHaikou 571101China Department of Biology Hainan Medical UniversityHaikouChina

Plant expression vectors are essential tools for gene functional analysis and molecular plant *** gene of interest is transferred to the vector by molecular cloning *** Cloning is a newly developed molecular cloning method with the advantages of simplicity,efficiency,and *** this study,we developed a"pNC"vector system that contains 55 Nimble Cloning-compatible vectors for functional analysis of genes in *** vectors contain the NC frame flanked by unique adapters for one-step and standardized Nimble *** demonstrate that the pNC vectors are convenient and effective for the functional analysis of plant genes,including the study of gene ectopic expression,protein subcellular localization,protein-protein interaction,gene silencing(RNAi),virus-induced gene silencing,promoter activity,and CRISPR-Cas9-mediated genome ***"pNC"vector system represents a high-throughput toolkit that can facilitate the large-scale analysis of plant functional genomics.

关键词： nimble cloning plant expression vector gene function ectopic expression genome editing

Functional Characterization of Pollen Specific non-coding mRNA-like RNA Gene zm401 from Zea mays

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Functional Characterization of Pollen Specific non-coding mR...