Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of“Candidatus Liberibacter asiaticus”in citrus

作     者：Shuai Wang Meixia Du Liting Dong Rongrong Qu Danlu Ran Juanjuan Ma Xuefeng Wang Lanzhen Xu Weimin Li Yongrui He Xiuping Zou 

作者机构：Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science CityCitrus Research InstituteSouthwest University/National Citrus Engineering Research CenterChongqingPeople’s Republic of China Key Laboratory for Northern UrbanAgriculture of Ministry of Agriculture and Rural AffairsBeijing University of AgricultureBeijingPeople’s Republic of China 

出 版 物：《Molecular Horticulture》 (分子园艺（英文）)

年 卷 期：2023年第3卷第1期

页      面：197-213页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：Open access funding provided by Shanghai Jiao Tong University supported by the National Key Research and Development Program of China(2021YFD1400800) the National Natural Sciences Foundation of China(31972393) the Earmarked Fund for China Agriculture Research System(CARS-27) 

主　　题：Citrus HLB CaLasSDE460 Transcriptome Ectopic expression 

摘      要：Citrus Huanglongbing(HLB),caused by Candidatus Liberibacter asiaticus(CaLas),is the most serious disease ***460 was previously characterized as a potential virulence factor of ***,the function and mechanism of CaLasSDE460 involved in CaLas against citrus is still ***,we showed that transgenic expression of CaLasSDE460 in Wanjincheng oranges(*** Osbeck)contributed to the early growth of CaLas and the development of *** the temperature increased from 25℃to 32℃,CaLas growth and symptom development in transgenic plants were slower than those in WT ***-seq analysis of transgenic plants showed that CaLasSDE460 affected multiple biological *** 25℃,transcription activities of the“Protein processing in endoplasmic reticulumand“Cyanoamino acid metabolismpathways increased while transcription activities of many pathways decreased at 32℃.124 and 53 genes,separately annotated to plant-pathogen interaction and MAPK signaling pathways,showed decreased expression at 32℃,compared with these(38 for plant-pathogen interaction and 17 for MAPK signaling)at 25℃.Several important genes(MAPKKK14,HSP70b,NCED3 and WRKY33),remarkably affected by CaLasSDE460,were ***,our data suggested that CaLasSDE460 participated in the pathogenesis of CaLas through interfering transcription activities of citrus defense response and this interfering was temperature-dependent.