A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants
作者机构：Key Laboratory of Biology and Genetic Resources of Tropical CropsSanya Research InstituteInstitute of Tropical Biosciences and BiotechnologyChinese Academy of Tropical Agricultural Science&Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan ProvinceHainan Institute of Tropical Agricultural ResourcesHaikou 571101China Department of BiologyHainan Medical UniversityHaikouChina
出 版 物：《Plant Communications》 (植物通讯（英文）)
年 卷 期：2023年第4卷第2期
页 面：65-76页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：the Hainan Provincial Natural Science Foundation of China(320RC706 and 322RC760) the Central Public-interest Scientific Institution Basal Research Fund(no.1630052022004)
主 题：nimble cloning plant expression vector gene function ectopic expression genome editing
摘 要：Plant expression vectors are essential tools for gene functional analysis and molecular plant *** gene of interest is transferred to the vector by molecular cloning *** Cloning is a newly developed molecular cloning method with the advantages of simplicity,efficiency,and *** this study,we developed apNCvector system that contains 55 Nimble Cloning-compatible vectors for functional analysis of genes in *** vectors contain the NC frame flanked by unique adapters for one-step and standardized Nimble *** demonstrate that the pNC vectors are convenient and effective for the functional analysis of plant genes,including the study of gene ectopic expression,protein subcellular localization,protein-protein interaction,gene silencing(RNAi),virus-induced gene silencing,promoter activity,and CRISPR-Cas9-mediated genome ***pNCvector system represents a high-throughput toolkit that can facilitate the large-scale analysis of plant functional genomics.
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