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World Journal of Gastroenterology 2008年第11期14卷 1785-1789页

作者： Li-Li Xing Zhen-Ning Wang Yong Zhang Ying-Ying Xu Juan Li Li Jiang Yang Luo Xue Zhang Department of Surgical Oncology and General'Surgery the First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China Department of Medical Genomics of China Medical University Shenyang 110001 Liaoning Province China

AIM： To explore whether cyclooxygenase 2 （COX-2） -765G〉C polymorphism is associated with susceptibility of colorectal cancer （CRC） and to evaluate the risk of colorectal cancer in relation to environmental exposures and polymorphism. METHODS： We conducted a case-control study of 137 patients with colorectal cancer and 199 cancerfree controls in northeast China. Multivariate logistic regression analysis was performed to calculate the adjusted odds ratio （OR） and 95% confidence interval （95% CI）. RESULTS： The -765G〉C polymorphism was not independently associated with CRC risk. However, risk associated with the polymorphism differed by smoking and body mass index （BMI）. Smoking and BMI associated risks were stronger among those with -765GG genotype, showing that smokers had a 2.682-fold greater risk of CRC than nonsmokers （51/43 vs 68/126, P = 0.006）. Compared to those with a normal body mass index （BMI 18.5-22.9）, those with overweight （BMI 23-24.9） had a 3.909-fold higher risk of CRC （OR = 3.909, 95% CI = 2.081-7.344; P 〈 0.001）, while those with obesity （BMI 〉 25） had a 2.031- fold higher risk of CRC （OR = 1.107, 95% CI = 1.107-3.726; P = 0.022）. is not associated with an increased risk of CRC, -765GG genotype appears to be related to an increased risk in the presence of smoking and higher BMI.

关键词： Colorectal cancer cyclooxygenase 2 Polymorphism Smoking Body mass index

Imrecoxib: a novel and selective cyclooxygenase 2 inhibitor with anti-inflammatory effect

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Acta Pharmacologica Sinica 2004年第7期25卷 81-85页

作者： Xiao-hong CHEN, Jin-ye BAI, Fang SHEN, Ai-ping BAI, Zong-ru GUO, Gui-fang CHENG2 Departmentof Pharmacology, Institute of Materia Medica, Chinese Academyof Medical Sciencesand Peking Union Medical College, Beijing 100050, China Department of Pharmacology Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

AIM: To investigate the inhibitory effect of imrecoxib, a synthetic compound of completely new structure, on cyclooxygenase 1 (COX-1) and 2 (COX-2) and its anti-inflammatory effect in vivo. METHODS: The inhibitory effects of imrecoxib on cyclooxygenase 1 and 2 were studied using whole cell assay with murine peritoneal mac- rophages induced by calcimycin and LPS. The inhibitory effects of imrecoxib on mRNA level of COX-1 and COX- 2 in human macrophage cell line U937 were detected by reverse transcription polymerase chain reaction (RT-PCR) analysis. Effects of imrecoxib on acute and chronic inflammation were evaluated in rat carrageenan induced edema model and rat adjuvant-induced arthritis model, respectively. RESULTS: Imrecoxib was found to inhibit COX-1 and COX-2 with IC50 value of 115±28 nmol/L and 18±4 nmol/L, respectively. Imrecoxib was shown to selectively and dose-dependently inhibit COX-2 mRNA level. Imrecoxib effectively inhibited carrageenan-induced acute in- flammation at the doses of 5, 10, and 20 mg·kg-1 ig and adjuvant-induced chronic inflammation at the doses of 10 and 20 mg·kg-1·d-1 ig. CONCLUSION: Imrecoxib is a novel and moderately selective COX-2 inhibitor that pos- sesses anti-inflammatory effect by inhibition of COX-2 mRNA expression.

关键词： imrecoxib cyclooxygenase 1 cyclooxygenase 2 cyclooxygenase inhibitors inflammation

Spatiotemporal expression of inducible nitric oxide synthase and cyclooxygenase 2 in the spinal cord during early stage sciatic nerve crush injury

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Neural Regeneration Research 2010年第10期5卷 747-751页

作者： Qiben Wang Linfeng Zheng Yinggui Xie Qinghong Huang He Huang Zhicheng Zeng Song Wu Department of Human Anatomy XiangNan University Chenzhou 423000 Hunan Province China Department of Radiology Shanghai First People's Hospital Medical College Shanghai Jiaotong University Shanghai 200080 China Department of Orthopaedics Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China Department of Human Anatomy and Neurobiology Xiangya School of Medicine Central South University Changsha 410013 Hunan Province China

BACKGROUND： Previous studies have shown that inducible nitric oxide synthase （iNOS） and cyclooxygenase 2 （COX-2） participate in inflammatory immune responses and neuropathic pain following peripheral nerve injury. However, few reports have addressed time-dependent expression of iNOS and COX-2 following peripheral nerve injury. OBJECTIVE： To investigate spatiotemporal expression of iNOS and COX-2 during early stage sciatic nerve crush ***, TIME AND SETTING： The randomized, controlled, animal experiment was performed at the Laboratory of Applied Anatomy, Department of Human Anatomy and Neurobiology, Central South University, China from September 2006 to September ***： Mouse anti-rat iNOS monoclonal antibody and goat anti-rat COX-2 monoclonal antibody （Transduction Laboratory, USA）, as well as biotinylated rabbit anti-mouse lgG and biotinylated rabbit anti-goat IgG （Santa Cruz Biotechnology, USA） were used in the present ***： A total of 48 healthy, adult, Sprague Dawley rats were randomly assigned to three groups. In the model group （n = 32）, crush injury to the right sciatic nerve was established using an artery clamp. The model group was further assigned to four subgroups according to survival time （6,12, 24, and 72 hours）, respectively （n = 8）. Sham surgery （n = 8） and normal control （n = 8） groups were also *** OUTCOME MEASURES： iNOS and COX-2 expression was detected in the L4-6 spinal cord with immunohistochemistry. Gray values of iNOS- and COX-2-postive cells in the anterior horn and posterior horn of spinal cord, as well as quantification of iNOS- and COX-2-positive cells in the anterior horn of spinal cord, were ***： iNOS and COX-2 expression gradually increased in the anterior horn and posterior horn of the spinal cord on the damaged side over time from 6 hours following sciatic nerve injury （P〈0.05） and peaked at 72 hours. Simultaneously, the number of iNOS- and COX-2-positive cells similarly increased

关键词： inducible nitric oxide synthase cyclooxygenase 2 sciatic nerve spinal cord peripheral nerve injury neural regeneration

Expression and Significance of cyclooxygenase 2 Gene in Lung Cancer

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2004年第4期24卷 326-328页

作者：邢丽华张珍祥徐永健张惠兰刘剑波 Research Laboratory of Respiratory Disease Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

To study the expression of cyclooxygenase 2 (COX-2) gene and its relationship with clinicopathological characteristics of lung cancer, expression of the COX-2 mRNA was evaluated by reverse transcription polymerase chain reaction (RT-PCR) in cancerous tissues and paired adjacent non-cancerous tissues from 56 patients and benign lesions from 12 patients. Our results showed that expression of COX-2 gene was detected in a significantly greater proportion of cancerous tissues (60.7 %) than adjacent noncancerous tissues (10.7 %, P2, P2 gene was higher in adenocarcinoma than in squamous carcinoma (P2 gene expression and patients' age, sex, histological type of tumors, differentiation degree and TNM stages (P>0.05). The up-regulation of COX-2 gene in lung cancer tissues especially in adenocarcinoma suggested that COX-2 may play a role in the lung carcinogenesis and COX-2 gene may serve as a potential therapeutic target in lung cancer.

关键词： lung cancer cyclooxygenase 2 gene

Aspirin inhibits the proliferation of tobacco-related esophageal squamous carcinomas cell lines through cyclooxygenase 2 pathway

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Chinese Medical Journal 2007年第23期120卷 2086-2091页

作者： ZHOU Qiao-zhi LIU Hai-bo DING Xin-chun LI Peng ZHANG Shu-tian YU Zhong-lin Department of Digestive Diseases Beijing Friendship Hospital Capital Medical University Beijing 100050 China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 1130101 China

Background Cigarette smoking has been verified as the risk factor of esophageal squamous cell carcinoma （ESCC）. Overexpression of cyclooxygenase 2 （COX-2） is shown in ESCC. The objective of this study was to investigate the effects of cigarette smoking ethanol extract （EE） on the proliferation of the human ESCC cell lines, and to explore the correlation between the proliferation rate of human ESCC cell lines and the expression pattern of COX-2. Whether aspirin can inhibit the proliferation of the ESCC cell lines pretreated with EE, and regulate the mRNA expression levels of COX-2 are also examined. Methods Two human ESCC cell lines were selected. EC109 was poorly differentiated and EC9706 was highly differentiated. EC109 and EC9706 were treated with EE and aspirin for different time course. The cell growth of ESCC was measured by MTT reduction assay and the expression of COX-2 was measured by RT-PCR and Western blot analysis. Results EE promoted the proliferation of EC109 and EC9706 in dose- and time-dependent manners. In the concentration range （10-100 pg/ml for EE） and in the time range （24-72 hours） after addition of EE, the cell proliferation was prominent in an up-scaled manner respectively. Aspirin could inhibit the proliferation of cell lines EC109 and EC9706 pretreated with EE for 5 hours, in a dose-dependent manner. In the concentration range （0.5-8.0 mmot/L for aspirin）, the cell growth inhibition was prominent in an up-scaled manner accordingly （P〈0.05）. The effect of EE on cell proliferation was correlated with the up-regulation of COX-2 gene. However, the cell growth inhibition of aspirin was correlated with the down-regulation of COX-2 gene. Conclusions EE can stimulate the proliferation of human ESCC cell lines EC109 and EC9706, most likely through up-regulating the expression of COX-2. Aspirin can inhibit the proliferation of ESCC cell lines induced by EE, which suggests it may be advantageous in the chemoprevention and therapy of human tobacco-relat

关键词： smoke esophageal neoplasms cyclooxygenase 2 aspirin

Quantitative analysis of cyclooxygenase 2 in the posterior longitudinal ligament of cervical spondylotic myelopathy

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Chinese Medical Journal 2011年第16期000卷 2480-2484页

作者： SONG Hong-xing Michael Scarpatetti Wolfgang Kreil SHEN Hui-liang Koppany Bodo Birgit Ebner Heribert Schrottner Michael Mokry Department of Orthopaedics Xuanwu Hospital Capital MedicalUniversity Beijing 100053 China Institute of Pathology Medical University of Graz 8036 Graz Austria Department of Neurosurgery Medical University of Graz 8036 Graz Austria Center for Medical Research Medical University of Graz 8010Graz Austria

Background Cervical spondylotic myelopathy （CSM）, in part, results from degeneration of the posterior longitudinal ligament （PLL）, which mechanically compresses the spinal cord. Much research was done on the ossification of PLL, but not concerning the non-ossifying degeneration of cervical PLL. The degeneration of cervical PLL may be related to inflammation. The aim of this study was to elucidate the pathological features of the PLL and the role of cyclooxygenase 2 （COX-2） in the degeneration of the PLL in CSM. Methods A total of 23 PLL specimens were collected during surgery from patients with CSM for the histological and immunohistochemical （type II collagen and Ki-67） study. For the control group 14 cervical PLL autopsy specimens were investigated in the same manner, mRNA expression of COX-2 was quantitatively measured by real-time reverse transcription-polymerase chain reaction （RT-PCR） from 18 PLL specimens of patients with CSM and 18 PLL specimens of autopsy cases. Immunohistochemistry was used to evaluate the cellular location of COX-2 in PLL. Results A distinct amount of fibrotic area, chondrometaplastic tissue and calcification were found in the PLL of the patient group, compared with the control group. Type II collagen was apparent around chondrometaplastic cells. Ki-67 positive reaction was less than 5%. A COX-2 positive reaction was found in 9 of the patient specimens （39.1%） in which the COX-2 was released from vascular endothelial cells in the PLL. However, such reactions were not found in the control group. Real-time PCR showed that the mRNA expression level of COX-2 in the patient group was significantly higher than that in the control group （P〈0.01）. Conclusions Chondrometaplastic tissue producing type II collagen was identified as the most predominant pathological feature in the degenerative PLL. The higher expression of COX-2 might be related to degeneration of the PLL in CSM.

关键词： posterior longitudinal ligament cyclooxygenase 2 immunohistochemistry polymerase chain reaction

Effects of electroacupuncture on uterine prostaglandin F2α,cyclooxygenase 2 and nuclear factorκB in rats with primary dysmenorrhea

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Journal of Acupuncture and Tuina Science 2020年第6期18卷 418-424页

作者： Liu Yu Tang Wen-jing Wang Yi-qin Tang Biao Medical School Hunan University of Chinese MedicineChangsha 410028China

Objective:To observe the effects of electroacupuncture(EA)on uterine prostaglandin F2α(PGF2α),cyclooxygenase 2(COX-2)and nuclear factorκB(NF-κB)in rats with primary dysmenorrhea(PD)and to discuss the possible mechanism in EA intervening ***:Forty Sprague-Dawley female rats were randomly divided into a blank group,a model group,an EA group and an ibuprofen group,with 10 rats in each *** PD model was established using estradiol benzoate combined with oxytocin in the model group,EA group and ibuprofen *** the same time of modeling,rats in the EA group were given EA at Guanyuan(CV 4)and Sanyinjiao(SP 6)once a day for 20 min each time for 10 consecutive *** was intragastrically administered once a day for 10 consecutive days in the ibuprofen *** same amount of normal saline was intragastrically administered once a day for 10 consecutive days in the blank group and model *** number of writhing of rats in each group within 30 min was compared on the 11th day just after the *** uterine homogenate supernatant was separated and the PGF2αlevel was detected by enzyme-linked immunosorbent *** blot was applied for the detection of the expression levels of COX-2,phospho-NF-κB p65 and NF-κB p65 proteins in uterine ***:Compared with the blank group,the number of writhing in the model group increased significantly(P2α,COX-2,phospho-NF-κB p65 and NF-κB p65 proteins in uterine tissues were significantly increased(all P2αand COX-2 protein in uterine tissues were significantly reduced(both P<0.01).Compared with the model group,the phospho-NF-κB p65 level in uterine tissues in the EA group was significantly reduced(P<0.01).Compared with the ibuprofen group,the phospho-NF-κB p65 level in the EA group was signif

关键词： Acupuncture Therapy Electroacupuncture Dysmenorrhea Prostaglandins F cyclooxygenase 2 NF-kappa B Rats

Expression of inducible nitric oxide synthase and cyclooxygenase-2 in pancreatic adenocarcinoma:Correlation with microvessel density

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World Journal of Gastroenterology 2004年第13期10卷 1918-1922页

作者： Hans U.Kasper Hella Wolf Uta Drebber Helmut K.Wolf Michael A.Kern Department of Pathology University of CologneGermany Department of Pathology University of MagdeburgGermany Department of Pathology Johannes-GutenbergUniversity of MainzGermany

AIM:Cydooxygenases (COX) are key enzymes for conversion of arachidonic acid to *** oxide synthase (NOS) is the enzyme responsible for formation of nitric oxide. Both have constitutive and inducible *** inducible isoforms (iNOS and COX-2) are of great interest as regulators of tumor angiogenesis,tumorigenesis and inflammatory *** study was to clarify their role in pancreatic adenocarcinomas. METHODS:We investigated the immunohistochemical iNOS and COX-2 expression in 40 pancreatic ductal adenocardnomas of different grade and *** results were compared with microvessel density and dinicopathological data. RESULTS:Twenty-one (52.5%) of the cases showed iNOS expression,15 (37.5%) of the cases were positive for COX-2. The immunoreaction was heterogeneously distributed within the *** intensity was different between the *** correlation between iNOS and COX-2 expression was *** was no relationship with microvessel density. However,iNOS positive tumors developed more often distant metastases and the more malignant tumors showed a higher COX-2 *** was no correlation with other clinicopathological data. CONCLUSION:Approximately half of the cases expressed iNOS and *** two enzymes do not seem to be the key step in angiogenesis or carcinogenesis of pancreatic *** to a low prevalence of COX-2 expression,chemoprevention of pancreatic carcinomas by COX-2 inhibitors can only achieve a limited success.

关键词： Adenocarcinoma Aged Aged, 80 and over cyclooxygenase 2 Female Humans Immunohistochemistry Isoenzymes Male Membrane Proteins Microcirculation Middle Aged Nitric Oxide Synthase Nitric Oxide Synthase Type II Pancreas Pancreatic Neoplasms Prostaglandin-Endoperoxide Synthases

Effect of Propyl Gallate on Activity of cyclooxygenase 1 and 2 in Mice's Peritoneal Macrophages*

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Chinese Journal of Integrated Traditional and Western Medicine 2004年第3期10卷 213-217页

作者：殷惠军蒋跃绒吴晓华陈晓红陈可冀 Xiyuan Hospital China Academy of Traditional Chinese Medcine Beijing (100091) China Fujian Mindong Rejuvenation Pharmaceutical Co. Ltd. Fuzhou Institute of Materia Medica Chinese Academy of Medical Sciences

Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages. Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F1α(6-keto-PGF1α) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term. Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF1α synthesis at the concentrations of 1×10-5, 5×10-6 mol/L (P>0.05), but enhanced 6-keto-PGF1α synthesis at the concentrations of 1×10-6, 5×10-7, 1×10-7 mol/L (P2-derived PGE2 synthesis at the concentrations of 1×10-5,1×10-6 mol/L (P2 at higher concentrations in murine peritoneal macrophages.

关键词： propyl gallate cyclooxygenase 1 cyclooxygenase 2 inflammation

Inhibitory Effect of 3,4-diaryl-3-pyrrolin-2-ones on cyclooxygenase 1 and 2 in Murine Peritoneal Macrophages

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Inhibitory Effect of 3,4-diaryl-3-pyrrolin-2-ones on Cycloox...