Effect of Propyl Gallate on Activity of Cyclooxygenase 1 and 2 in Mice's Peritoneal Macrophages*

作     者：殷惠军 蒋跃绒 吴晓华 陈晓红 陈可冀 

作者机构：Xiyuan Hospital China Academy of Traditional Chinese Medcine Beijing (100091) China Fujian Mindong Rejuvenation Pharmaceutical Co. Ltd. Fuzhou Institute of Materia Medica Chinese Academy of Medical Sciences 

出 版 物：《Chinese Journal of Integrated Traditional and Western Medicine》 (中国中西医结合杂志（英文版）)

年 卷 期：2004年第10卷第3期

页      面：213-217页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：ThisworkissupportedbytheNationalNaturalScienceFoundation(itemnumber:90209054) 

主　　题：propyl gallate cyclooxygenase 1 cyclooxygenase 2 inflammation 

摘      要：Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages. Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F1α(6-keto-PGF1α) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term. Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF1α synthesis at the concentrations of 1×10-5, 5×10-6 mol/L (P0.05), but enhanced 6-keto-PGF1α synthesis at the concentrations of 1×10-6, 5×10-7, 1×10-7 mol/L (P0.01) in vitro, and showed a good dose-dependent manner. It inhibited LPS-induced, COX-2-derived PGE2 synthesis at the concentrations of 1×10-5,1×10-6 mol/L (P 0. 05). Conclusion: Within the range of 1×10-5 to 1×10-7 mol/L, PrG activated COX-1 at lower concentrations and inhibited COX-2 at higher concentrations in murine peritoneal macrophages.