Spatiotemporal expression of inducible nitric oxide synthase and cyclooxygenase 2 in the spinal cord during early stage sciatic nerve crush injury

作     者：Qiben Wang Linfeng Zheng Yinggui Xie Qinghong Huang He Huang Zhicheng Zeng Song Wu 

作者机构：Department of Human Anatomy XiangNan University Chenzhou 423000 Hunan Province China Department of Radiology Shanghai First People's Hospital Medical College Shanghai Jiaotong University Shanghai 200080 China Department of Orthopaedics Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China Department of Human Anatomy and Neurobiology Xiangya School of Medicine Central South University Changsha 410013 Hunan Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2010年第5卷第10期

页      面：747-751页

核心收录：

学科分类：10[医学] 

基　　金：the National Natural Science Foundation of China,No. 30860290 the Scientific Research Foundation of Department of Health of Hunan Province of China,No. C2007038 the Scientific Research Program of Department of Education of Hunan Province,No. 08C816 the Nanometer Specific Foundation of Shanghai of China,No. 1052nm05800 the Doctor Innovation Foundation of Medical College of Shanghai Jiao Tong University of China,No. BXJ201043 

主　　题：inducible nitric oxide synthase cyclooxygenase 2 sciatic nerve spinal cord peripheral nerve injury neural regeneration 

摘      要：BACKGROUND： Previous studies have shown that inducible nitric oxide synthase （iNOS） and cyclooxygenase 2 （COX-2） participate in inflammatory immune responses and neuropathic pain following peripheral nerve injury. However, few reports have addressed time-dependent expression of iNOS and COX-2 following peripheral nerve injury. OBJECTIVE： To investigate spatiotemporal expression of iNOS and COX-2 during early stage sciatic nerve crush ***, TIME AND SETTING： The randomized, controlled, animal experiment was performed at the Laboratory of Applied Anatomy, Department of Human Anatomy and Neurobiology, Central South University, China from September 2006 to September ***： Mouse anti-rat iNOS monoclonal antibody and goat anti-rat COX-2 monoclonal antibody （Transduction Laboratory, USA）, as well as biotinylated rabbit anti-mouse lgG and biotinylated rabbit anti-goat IgG （Santa Cruz Biotechnology, USA） were used in the present ***： A total of 48 healthy, adult, Sprague Dawley rats were randomly assigned to three groups. In the model group （n = 32）, crush injury to the right sciatic nerve was established using an artery clamp. The model group was further assigned to four subgroups according to survival time （6,12, 24, and 72 hours）, respectively （n = 8）. Sham surgery （n = 8） and normal control （n = 8） groups were also *** OUTCOME MEASURES： iNOS and COX-2 expression was detected in the L4-6 spinal cord with immunohistochemistry. Gray values of iNOS- and COX-2-postive cells in the anterior horn and posterior horn of spinal cord, as well as quantification of iNOS- and COX-2-positive cells in the anterior horn of spinal cord, were ***： iNOS and COX-2 expression gradually increased in the anterior horn and posterior horn of the spinal cord on the damaged side over time from 6 hours following sciatic nerve injury （P〈0.05） and peaked at 72 hours. Simultaneously, the number of iNOS- and COX-2-positive cells similarly increased