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High matrix metalloproteinase-9 expression induces angiogenesis and basement membrane degradation in stroke-prone spontaneously hypertensive rats after cerebral infarction

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Neural Regeneration Research 2014年第11期9卷 1154-1162页

作者： Huilian Hou Guanjun Zhang Hongyan Wang Huilin Gong Chunbao Wang Xuebin Zhang Department of Pathology the First Affiliated Hospital of Medical School of Xi’an Jiaotong University

Basement membrane degradation and blood-brain barrier damage appear after cerebral infarc- tion, severely impacting neuronal and brain functioning; however, the underlying pathogenetic mechanisms remain poorly understood. In this study, we induced cerebral infarction in stroke- prone spontaneously hypertensive rats by intragastric administration of high-sodium water （1.3% NaC1） for 7 consecutive weeks. Immunohistochemical and immunofluorescence assays demonstrated that, compared with the non-infarcted contralateral hemisphere, stroke-prone spontaneously hypertensive rats on normal sodium intake and Wistar-Kyoto rats, matrix metalloproteinase-9 expression, the number of blood vessels with discontinuous collagen iv expression and microvessel density were significantly higher, and the number of continuous collagen iv-positive blood vessels was lower in the infarct border zones of stroke-prone sponta- neously hypertensive rats given high-sodium water. Linear correlation analysis showed matrix metalloproteinase-9 expression was positively correlated with the number of discontinuously collagen iv-labeled blood vessels and microvessel density in cerebral infarcts of stroke-prone spontaneously hypertensive rats. These results suggest that matrix metalloproteinase-9 upregula- tion is associated with increased regional angiogenesis and degradation of collagen iv, the major component of the basal lamina, in stroke-prone spontaneously hypertensive rats with high-sodi- um water-induced focal cerebral infarction.

关键词： nerve regeneration cerebral infarction matrix metalloproteinase-9 collagen iv microvessel density angiogenesis basement membrane degradation high sodium stroke-pronespontaneously hypertensive China Medical Board Project neural regeneration

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Hydrogen selenide,a vital metabolite of sodium selenite,uncouples the sulfilimine bond and promotes the reversal of liver fibrosis

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Science China(Life Sciences) 2021年第3期64卷 443-451页

作者： Dongrui Luan Zengteng Zhao Dandan Xia Qiuling Zheng Xiaonan Gao Kehua Xu Bo Tang College of Chemistry Chemical Engineering and Materials ScienceCollaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of ShandongKey Laboratory of Molecular and Nano ProbesMinistry of EducationInstitute of Molecular and Nano ScienceShandong Normal UniversityJinan 250014China Department of Pharmaceutical Analysis School of PharmacyState Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjing 210009China

Sodium selenite has alleviating effects on liver fibrosis;however,its therapeutic molecular mechanism remains ***,hydrogen selenide,a major metabolite of Na_(2)SeO_(3),was tested to uncouple the sulfilimine bond in collagen iv,the biomarker of liver fibrosis.A mouse model of liver fibrosis was constructed via a CCl_(4)-induced method,followed by the administration of 0.2 mg kg−1 Na_(2)SeO_(3)via gavage three times per week for 4 *** in H2Se,NADPH,and H_(2)O_(2)levels were monitored in real time by using NIR-H2Se,DCI-MQ-NADPH,and H_(2)O_(2)probes in vivo,respectively.H_(2)Se continuously accumulated in the liver throughout the Na_(2)SeO_(3)treatment period,but the levels of NADPH and H_(2)O_(2)*** expression of collagen iv was analyzed through Western blot and liquid chromatography-mass *** confirmed that the sulfilimine bond of collagen iv in the fibrotic mouse livers could be broken by H2Se with the Na_(2)SeO_(3)***,the therapeutic effect of Na_(2)SeO_(3)on liver fibrosis could be mainly attributed to H_(2)Se that uncoupled the sulfilimine bond to induce collagen iv *** study provided a reasonable explanation for the molecular mechanism of the in vivo function of Na_(2)SeO_(3)and the prevention of liver fibrosis by administering inorganic selenium.

关键词： hydrogen selenide sodium selenite sulfilimine bond liver fibrosis reversal collagen iv

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Distribution of pericellular matrix molecules in the temporomandibular joint and their chondroprotective effects against inflammation

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International Journal of Oral Science 2017年第1期9卷 43-52页

作者： Wern Cui Chu Shipin Zhang Timothy J Sng Yu Jie Ong Wen-Li Tan Vivien Y Ang Casper B Foldager Wei Seong Toh Faculty of Dentistry National University of SingaporeSingapore Orthopaedic Research Laboratory Aarhus University HospitalAarhusDenmark Tissue Engineering Program Life Sciences InstituteNational University of SingaporeSingapore

The objectives of this study were to （1） determine the distribution and synthesis of pericellular matrix （PCM） molecules （collagen VI, collagen iv and laminin） in rat temporomandibular joint （TMJ） and （2） investigate the effects of PCM molecules on chondrocytes against inflammation in osteoarthritis. Four zones （fibrous, proliferating, mature and hypertrophic） of condylar cartilage and three bands （anterior, intermediate and posterior） of disc were analysed by immunohistochemistry for the presence of PCM molecules in rat TMJs. Isolated chondrocytes were pre-treated with PCM molecules before being subjected to interleukin （IL）-II~ treatment to stimulate inflammation. The responses of the chondrocytes were analysed using gene expression, nitric oxide release and matrix metalloproteinase （MMP）-13 production measures. Histomorphometric analyses revealed that the highest areal deposition of collagen VI （67.4%）, collagen iv （45.7%） and laminin （52.4%） was in the proliferating zone of TMJ condylar cartilage. No significant difference in the distribution of PCM molecules was noted among the three bands of the TMJ disc. All three PCM molecules were expressed intracellularly by chondrocytes cultured in the monolayer. Among the PCM molecules, pre-treatment with collagen VI enhanced cellular proliferation, ameliorated IL-lp-induced MMP-3, MMP-9, MMP-13 and inducible nitric oxide synthase gene expression, and attenuated the downregulation of cartilage matrix genes, including collagen I, aggrecan and cartilage oligomeric matrix protein （COMP）. Concurrently, collagen VI pretreatment inhibited nitric oxide and MMP-13 production. Our study demonstrates for the first time the distribution and role of PCM molecules, particularly collagen VI, in the protection of chondrocytes against inflammation.

关键词： cartilage chondrocytes collagen iv collagen W inflammation laminin pericellular matrix temporomandibular joint

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The development of blood-retinal barrier during the interaction of astrocytes with vascular wall cells

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Neural Regeneration Research 2014年第10期9卷 1047-1054页

作者： Huanling Yao Tianshi Wang Jiexin Deng Ding Liu Xiaofei Li Jinbo Deng Institute of Neurobiology College of Life ScienceHenan University

Astrocytes are intimately involved in the formation and development of retinal vessels. Astrocyte dysfunction is a major cause of blood-retinal barrier injury and other retinal vascular diseases. In this study, the development of the retinal vascular system and the formation of the blood-ret-inal barrier in mice were investigated using immunolfuorescence staining, gelatin-ink perfusion, and transmission electron microscopy. The results showed that the retinal vascular system of mice develops from the optic disc after birth, and radiates out gradually to cover the entire retina, taking the papilla optica as the center. First, the superifcial vasculature is formed on the inner retinal layer;then, the vasculature extends into the inner and outer edges of the retinal inner nuclear layer, forming the deep vasculature that is parallel to the superifcial vasculature. The blood-retinal barrier is mainly composed of endothelium, basal lamina and the end-feet of astrocytes, which become mature during mouse development. Initially, the naive endothelial cells were immature with few organelles and many microvilli. The basal lamina was uniform in thickness, and the glial end-feet surrounded the outer basal lamina incompletely. In the end, the blood-retinal barrier matures with smooth endothelia connected through tight junctions, rela-tively thin and even basal lamina, and relatively thin glial cell end-feet. These ifndings indicate that the development of the vasculature in the retina follows the rules of“center to periphery”and“superifcial layer to deep layers”. Its development and maturation are spatially and tempo-rally consistent with the functional performance of retinal neurons and photosensitivity. The blood-retinal barrier gradually becomes mature via the process of interactions between astro-cytes and blood vessel cells.

关键词： nerve regeneration retina growth development blood vessels blood-retinal barrier astrocytes immunofluorescence ultrastructure mouse collagen iv glial fibrillary acidic protein NSFC grant neural regeneration

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Effects of Zao Huang Mixture (藻黄合剂) on the Expressions of TGF-β1 and Col iv in Human Glomerular Mesangial Cells Cultured in High Glucose Environment

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Journal of Traditional Chinese Medicine 2011年第2期31卷 127-129页

作者：鞠建伟牟授菡尹燕志吕丛奎孙晓燕邓磊修 Yantai Economy and Technology Development Zone Hospital Shandong Simcere-Medgenn Bio- pharmaceutical Company

Objective: To investigate the effect of Zao Huang Mixture (藻黄合剂ZHM) on expressions of growth factor-β1 (TGF-β1) and collagen iv (Col iv) in human glomerular mesangial cells (GMC) cultured in high-glucose environment. Methods: After primary culture of GMC, in vitro culture was carried out in normal group, high glucose group and high glucose medium with ZHM of different concentrations, and the expressions of TGF-β1 and Col iv in the GMC group and in ZHM group were detected at 24 and 48 h respectively. Results: Compared with the normal group, expressions of TGF-β1 and Col iv significantly increased at 24 h, 48 h in the high glucose group (all Piv in all the ZHM groups significantly decreased at 24 h, 48 h (Piv in glomerular mesangial cells.

关键词： Zao Huang Mixture (藻黄合剂JZHM) transforming growth factor-ill collagen iv human glomerular mesangial cells high glucose environment

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