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cassava Me RS40 is required for the regulation of plant salt tolerance

Journal of Integrative Agriculture 2023年第5期22卷 1396-1411页

作者： MA Xiao-wen MA Qiu-xiang MA Mu-qing CHEN Yan-hang GU Jin-bao LI Yang HU Qing LUO Qing-wen WEN Ming-fu ZHANG Peng LI Cong WANG Zhen-yu Institute of Nanfan&Seed Industry Guangdong Academy of SciencesGuangzhou 510316P.R.China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032P.R.China Zhanjiang Research Center Institute of Nanfan&Seed IndustryGuangdong Academy of SciencesZhanjiang 524300P.R.China

Soil salinity affects the expression of serine/arginine-rich(SR) genes and isoforms by alternative splicing, which in turn regulates the adaptation of plants to *** previously identified the cassava spliceosomal component 35 like(SCL) and SR subfamilies, belonging to the SR protein family, which are extensively involved in responses to abiotic ***, the post-transcriptional regulatory mechanism of cassava arginine/serine-rich(RS) subfamily in response to salt stress remains to be *** the current study, we identified 37 genes of the RS subfamily from 11 plant species and systematically investigated the transcript levels of the RS40 and RS31 genes under diverse abiotic stress ***, an analysis of the conserved protein domains revealed that plant RS subfamily genes were likely to preserve their conserved molecular functions and played critical functional roles in responses to abiotic ***, we found that overexpression of MeRS40 in Arabidopsis enhanced salt tolerance by maintaining reactive oxygen species homeostasis and up-regulating the salt-responsive ***,overexpression of MeRS40 gene in cassava reduced salt tolerance due to the depression of its endogenous gene expression by negative autoregulation of its own ***, the MeRS40 protein interacted with MeU1-70Ks(MeU1-70Ka and MeU1-70Kb) in vivo and in vitro, ***, our findings highlight the critical role of cassava SR proteins in responses to salt stress in plants.

关键词： cassava alternative splicing serine/arginine-rich proteins salt stress

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Optimization of hydrothermal pretreatment for co-utilization of xylose and glucose of cassava anaerobic residue for producing ethanol

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Chinese Journal of Chemical Engineering 2019年第4期27卷 920-927页

作者： Huisheng Lü Jinyi Zhou Jiatao Liu Chunliu Lü Feng Lian Yonghui Li Key Laboratory for Green Chemical Technology of Ministry of Education R&D Center for Petrochemical Technology Tianjin University Tianjin 300072 China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072 China

The development of a process that could recover biofuel from industrial cellulose waste can not only reduce the negative environmental impacts by using fossil fuels, but also bring a green idea for the waste’s disposing. In this study, hydrothermal pretreatment was optimized for cassava anaerobic residue, a cellulosic waste from cassava ethanol industry, to co-utilize xylose and glucose for producing bioethanol. The effect of the main pretreatment conditions, namely, temperature, solid content and time, was explored for the highest recovery of xylose in prehydrolysate and glucose in enzymatic hydrolysate. The single factor experiment results showed that the conditions for maximum xylose recovery in prehydrolysate and glucose recovery in enzymatic hydrolysate were 60℃, 75 min, 10% solids and 160℃, 75 min, 10% solids, respectively. Whereafter, response surface methodology(RSM) was applied to further optimize the pretreatment conditions for the maximum theoretical ethanol production through utilizing both xylose and glucose. A treatment at 163℃, for 59 min and with 9.5%solids was found optimal, with the highest ethanol production of 20.2 mg·g^-1 raw material. Furthermore, in order to assess the impacts of the pretreatment on cassava anaerobic residue, the changes in crystallinity and morphology for untreated and pretreated solids were investigated.

关键词： Waste treatment Biofuel Hydrothermal cassava anaerobic residue Co-utilization Response surface methodology

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Optimization and modeling of biohydrogen production by mixed bacterial cultures from raw cassava starch

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Frontiers of Chemical Science and Engineering 2017年第1期11卷 100-106页

作者： Shaojie Wang Zhihong Ma Ting Zhang Meidan Bao Haijia Su Beijing Key Laboratory of Bioprocess Beijing University of Chemical Technology Beijing 100029 China

The production of bio-hydrogen from raw cassava starch via a mixed-culture dark fermentation process was investigated. The production yield of H2 was optimized by adjusting the substrate concentration and the microorganism mixture ratio. A maximum H2 yield of 1.72 mol H2/mol glucose was obtained with a cassava starch concentration of 10 g/L to give a 90% utilization rate. The kinetics of the substrate utilization and of the generation of both hydrogen and volatile fatty acids were also investigated. The substrate utilization follows pseudo first order reaction kinetics, whereas the production of both H2 and the VFAs correlate with the Gompertz equation. These results show that cassava is a good candidate for the production of biohydrogen.

关键词： cassava biohydrogen mixed cultures,kinetics

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A homeodomain-leucine zipper I transcription factor, MeHDZ14,regulates internode elongation and leaf rolling in cassava(Manihot esculenta Crantz)

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The Crop Journal 2023年第5期11卷 1419-1430页

作者： Xiaoling Yu Xin Guo Pingjuan Zhao Shuxia Li Liangping Zou Wenbin Li Ziyin Xu Ming Peng Mengbin Ruan National Key Laboratory for Tropical Crop Breeding Sanya 572025HainanChina Key Laboratory of Biology and Genetic Resources of Tropical Crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101HainanChina Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province Hainan Institute for Tropical Agricultural ResourcesHaikou 571101HainanChina Sanya Research Institute Chinese Academy of Tropical Agricultural SciencesSanya 572025HainanChina College of Tropical Crops Hainan UniversityHaikou 570228HainanChina

Drought stress impairs plant growth and other physiological functions. MeHDZ14, a homeodomainleucine zipper I transcription factor, is strongly induced by drought stress in various cassava ***, the role of MeHDZ14 in cassava growth regulation has remained unclear. Here we report that MeHDZ14 affected plant height, such that a dwarf phenotype and altered internode elongation were observed in transgenic cassava lines. MeHDZ14 was found to negatively regulate the biosynthesis of lignin. Its overexpression resulted in abaxially rolled leaves. The morphogenesis of leaf epidermal cells was inhibited by overexpression of MeHDZ14, with decreased auxin and gibberellin and increased cytokinin contents. MeHDZ14 was found to regulate many drought-responsive genes, including genes involved in cell wall synthesis and expansion. MeHDZ14 bound to the promoter of caffeic acid 3-Omethyltransferase 1(MeCOMT1), acting as a transcriptional repressor of genes involved in cell wall development. MeHDZ14 appears to act as a negative regulator of internode elongation and epidermal cell morphogenesis during cassava leaf development.

关键词： HD-Zip transcription factor Drought Internode elongation Leaf rolling cassava

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A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression

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Journal of Integrative Agriculture 2022年第9期21卷 2588-2602页

作者： DONG Shi-man XIAO Liang LI Zhi-bo SHEN Jie YAN Hua-bing LI Shu-xia LIAO Wen-bin PENG Ming Key Laboratory of Biology and Genetic Resources of Tropical Crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101P.R.China Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province Hainan Institute for Tropical Agricultural ResourcesHaikou 571101P.R.China Cash Crops Research Institute Guangxi Academy of Agricultural SciencesNanning 530007P.R.China College of Tropical Crops Hainan UniversityHaikou 570228P.R.China

cassava is an important tropical cash crop. Severe drought stresses affect cassava productivity and quality, and cause great economic losses in agricultural production. Enhancing the drought tolerance of cassava can effectively improve its yield. Long non-coding RNAs(lncRNAs) are present in a wide variety of eukaryotes. Recently, increasing evidence has shown that lncRNAs play a critical role in the responses to abiotic stresses. However, the function of cassava lncRNAs in the drought response remains largely unknown. In this study, we identified a novel lncRNA, DROUGHT-INDUCED INTERGENIC lncRNA(DIR). Gene expression analysis showed that DIR was significantly induced by drought stress treatment, but did not respond to abscisic acid(ABA) or jasmonic acid(JA) treatments. In addition, overexpression of the DIR gene enhanced proline accumulation and drought tolerance in transgenic cassava. RNA-seq analysis revealed that DIR preferentially affected drought-related genes that were linked to transcription and metabolism. Moreover, RNA pull-down mass spectrometry analysis showed that DIR interacted with 325 proteins. A protein–protein interaction(PPI)analysis found a marked enrichment in proteins associated with the mRNA export and protein quality control ***, these results suggest that DIR and its interacting proteins that regulate mRNA or protein metabolism are involved in mediating the drought stress response. Thus, regulating DIR expression has potential for improving cassava yield under drought conditions.

关键词： lncRNA RNA-seq drought stress cassava DIR

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Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life,Increases Drought Stress Resistance and Alters Cytokinin Metabolism in cassava

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Journal of Integrative Plant Biology 2010年第7期52卷 653-669页

作者： Peng Zhang Wen-Quan Wang Gen-Liang Zhang Miroslav Kaminek Petre Dobrev Jia Xu Wilhelm Gruissem Shanghai Center for cassava Biotechnology National Laboratory of Plant Molecular Genetics Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Department of Biology Plant Biotechnology ETH Zurich Universitatstrasse 2 8092 Zurich Switzerland Institute of Tropical Biosciences and Biotechnology Chinese Academy of Tropical Agricultural Science Haikou 571101 China Institute of Experimental Botany Academy of Sciences of the Czech Republic Rozvojova 263 CZ-165 02 Prague 6 Czech Republic Key Laboratory of Synthetic Biology Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

cassava （Manihot esculenta Crantz） sheds its leaves during growth, especially within the tropical dry season. With the production of SAG12-1PT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants. Physiological and agronomical characterizations of these plants also revealed that the induced expression of IPT had effects on photosynthesis, sugar allocation and nitrogen partitioning. Importantly, the 529-28 plants accumulated a high level of trans-zeatin-type cytokinins particularly of corresponding storage O-glucosides to maintain cytokinin homeostasis. Our study proves the feasibility of prolonging the leaf life of woody cassava and also sheds light on the control of cytokinin homeostasis in cassava leaves.

关键词： cassava MANIHOT PLANT growth CYTOKININS TRANSGENIC plants PLANT-water relationships

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Optimization of enzymatic hydrolysis of cassava to obtain fermentable sugars

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第7期13卷 579-586页

作者： Renata M.COLLARES Luiza V.S.MIKLASEVICIUS Mariana M.BASSACO Nina P.G.SALAU Marcio A.MAZUTTI Dilson A.BISOGNIN Lisiane M.TERRA Department of Chemical Engineering Federal University of Santa MariaAv.Roraima 1000Santa Maria 97105-900Brazil Department of Horticulture Federal University of Santa MariaAv.Roraima 1000Santa Maria 97105-900Brazil

This work evaluates the enzymatic hydrolysis of starch from cassava using pectinase,α-amylase,and amyloglucosidase.A central composite rotational design(CCRD)was carried out to evaluate the effects of amyloglucosidase,pectinase,reaction time,and solid to liquid *** the experiments were carried out in a bioreactor with working volume of 2 *** 98%efficiency hydrolysis was obtained,resulting in a concentration of total reducing sugar released of 160 g/*** was concluded that pectinase improved the hydrolysis of starch from *** time was found to be significant until 7 h of reaction.A solid to liquid ratio of 1.0 was considered suitable for hydrolysis of starch from *** was a significant variable in the process:after its addition to the reaction media,a 30%-50%increase in the amount of total reducing sugar released was *** optimal conditions the maximum productivity obtained was 22.9 g/(L.h).

关键词： cassava Enzymatic hydrolysis Enzyme reactor Starch

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Expression Profiling of cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis

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Journal of Integrative Plant Biology 2011年第3期53卷 193-211页

作者： Jun Yang Dong An Peng Zhang Shanghai Center for cassava Biotechnology National Laboratory of Plant Molecular Genetics Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Key Laboratory of Synthetic Biology Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Shanghai Chenshan Plant Science Research Center the Chinese Academy of Sciences Chenshan Botanical Garden Shanghai 201602 China

Mechanisms related to the development of cassava storage roots and starch accumulation remain largely unknown. To evaluate genome-wide expression patterns during tuberization, a 60 mer oligonucleotide microarray representing 20 840 cassawl genes was designed to identify differentially expressed transcripts in fibrous roots, developing storage roots and mature storage roots. Using a random variance model and the traditional twofold change method for statistical analysis, 912 and 3 386 upregulated and downregulated genes related to the three developmental phases were identified. Among 25 significantly changed pathways identified, glycolysis/gluconeogenesis was the most evident one. Rate-limiting enzymes were identified from each individual pathway, for example, enolase, L-lactate dehydrogenase and aldehyde dehydrogenase for glycolysis/gluconeogenesis, and ADP-glucose pyrophosphorylase, starch branching enzyme and glucan phosphorylase for sucrose and starch metabolism. This study revealed that dynamic changes in at least 16% of the total transcripts, including transcription fac- tors, oxidoreductases/transferases/hydrolases, hormone-related genes, and effectors of homeostasis. The reliability of these differentially expressed genes was verified by quantitative real-time reverse transcription-polymerase chain reaction. Tlhese studies should facilitate our understanding of the storage root formation and cassava improvement.

关键词： cassava ROOTS (Botany) -- Formation GLYCOLYSIS GLUCONEOGENESIS LACTATE dehydrogenase REVERSE transcriptase polymerase chain reaction PLANT gene expression OLIGONUCLEOTIDE arrays

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Identification and characterization of MeERF genes and their targets in pathogen response by cassava(Manihot esculenta)

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The Crop Journal 2021年第5期9卷 1145-1153页

作者： Yuhui Hong Yong Xiao Na Song Shousong Zhu Rui Zhao Ke Li Mengting Geng Xiaohui Yu Honggang Wang Wei Xia Yinhua Chen Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource College of Tropical CropsHainan UniversityHaikou 570228HainanChina Coconut Research Institute Chinese Academy of Tropical Agricultural SciencesWenchang 571339HainanChina School of Bioengineering Dalian University of TechnologyDalian 116024LiaoningChina

cassava,Manihot esculenta Crantz (Me),is a major dietary source of calories for over 700 million people in tropical *** production of cassava is constantly threatened by cassava bacterial blight (CBB),caused by Xanthomonas axonopodis *** (Xam).The gene resources for CBB-resistant breeding of cassava are *** model plant species,ethylene response factors play important roles in response to pathogen *** this study,cassava ethylene response factors (MeERFs) were identified and characterized as the first step in studying their potential for CBB-resistant breeding of *** the cassava genome 155 MeERFs were identified,of which 23 were induced by Xam *** promoter regions of204 genes harbored GCC-box that had the potential to interact with *** 37 transcriptomes derived from Xam infection treatment,four gene co-expression modules for the MeERFs and GCC-box containing genes were *** MeERFs were associated with two GCC-box containing genes:transcription initiation factor TFIIE subunit beta (MeTFIIE),and histone-lysine N-methyltransferase ASHR1 (MeASHR1).Dual-luciferase reporter assays showed that MeERF10 and MeERF58 positively regulated Me TFIIE;MeERF137 negatively regulated Me TFIIE;MeERF10 and MeERF137 positively regulated Me ASHR1;and MeERF35 negatively regulated Me *** four MeERFs may mediate pathogen response by regulating the expression of the two GCC-box containing genes.

关键词： cassava Ethylene response factor Expression profile Co-expression analysis Pathogen

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The aquaporin MePIP2;7 improves MeMGT9-mediated Mg2+ acquisition in cassava

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Journal of Integrative Plant Biology 2023年第10期65卷 2349-2367页

作者： Qiuxiang Ma Yancai Feng Shu Luo Lu Cheng Weijing Tong Xinlu Lu Youzhi Li Peng Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/College of Life Science and Technology Guangxi UniversityNanning 530004China

Aquaporins are important transmembrane water transport proteins which transport water and several neutral molecules. However, how aquaporins are involved in the synergistic transport of Mg2+and water remains poorly understood. Here, we found that the cassava aquaporin Me PIP2;7 was involved in Mg2+transport through interaction with Me MGT9, a lower affinity magnesium transporter protein. Knockdown of Me PIP2;7 in cassava led to magnesium deficiency in basal mature leaves with chlorosis and necrotic spots on their edges and starch over-accumulation. Mg2+content was significantly decreased in leaves and roots of Me PIP2;7-RNA interference(PIP-Ri) plants grown in both field and Mg2+-free hydroponic solution. Xenopus oocyte injection analysis verified that Me PIP2;7 possessed the ability to transport water only and Me MGT9 was responsible for Mg^(2+)*** importantly, Me PIP2;7 improved the transportability of Mg^(2+)via Me MGT9 as verified using the CM66 mutant complementation assay and Xenopus oocytes expressing system. Yeast twohybrid, bimolecular fuorescence complementation,co-localization, and co-immunoprecipitation assays demonstrated the direct protein–protein interaction between Me PIP2;7 and Me MGT9 in vivo. Mg2+fux was significantly elevated in Me PIP2;7-overexpressing lines in hydroponic solution through non-invasive micro-test technique analysis. Under Mg^(2+)-free condition, the retarded growth of PIP-Ri transgenic plants could be recovered with Mg^(2+)supplementation. Taken together, our results demonstrated the synergistic effect of the Me PIP2;7 and Me MGT9 interaction in regulating water and Mg2+absorption and transport in cassava.

关键词： aquaporin cassava magnesium transporter nutrient utilization protein interaction

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